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Biomedical subjects

F Cesselin

Publications and source records attributed to F Cesselin.

At least 145 records · Page 8Linked to original sources
PubMed1978

Circadian and circannual rhythms in plasma hormones and other variables of five healthy young human males.

Every other month (plasma) and every month (urine) circadian rhythms were documented during the course of 14 months. Annual changes were validated in the 24 h mean of: plasma FSH (annual crest time: February), LH (March), thyroxine (September), cortisol (February), renin activity (April), testosterone (October), urinary 17-hydroxycorticosteroids (March), aldosterone (March), potassium (May) as well as sexual activity (September) [self-recorded daily]. Plasma prolactin did not show an annual variation. In addition, annual changes in the circadian acrophase (crest time location in the 24 h scale) occurred for some of the documented variables: plasma thyroxine, cortisol, renin activity, testosterone, urinary aldosterone and potassium.

17-Hydroxycorticosteroids↗
PubMed1977

Radioimmunological determination of urinary tetrahydroaldosterone.

A method is presented for radioimmunological determination of 3alpha, 5beta-tetrahydroaldosterone. It is based upon the reactivity of this steroid with an antiserum induced by the 3-carboxymethyloxime of 18, 21-aldosterone diacetate conjugated with bovine serum albumin. One hundred microliters of urine enzymatically hydrolyzed with an helix pomatia preparation, containing tritiated tetrahydroaldosterone for the yield calculation, were extracted with dichloromethane and chromatographed on a small celite column. The yield after extraction and chromatography was 64 +/- 17%. The radioimmunological determination was carried out in a conventional manner. The method is specific, sensitive (10 pg/tube), exact, reproducible, very simple and extremely rapid. The results showed good agreement with values given by a colorimetric method (p less than 0.001). The median value measured in 45 healthy adult subjects under standard sodium diet was 53.3 microgram/24h (95 % of the population within a 16.6 to 131.1 microgram/24h range). In 78 cases of adrenocortical insufficiency, 60 cases of obesity and 28 cases of hypokalemia, the median values (and the ranges : microgram/24h) were respectively 7.7 (1.0 - 51.0), 80.9 (17.0 - 503.0) and 64.3 (8.0 - 181.0). In 330 hypertensive patients the excretion of tetrahydroaldosterone exceeded the normal range in 115 cases (35%) with a median of 199.7 microgram/24h (131 to 620 microgram/24h).

Aldosterone↗
PubMed1976

[Properties of anti-LH-RH serums induced by 2 different immunogens].

Several anti-LH RH antisera have been raised in Rabbits given as immunogen the synthetic decapeptide linked to bovine serum albumin by carbodiimide or bis-diazotized benzidine. Antibodies obtained with CDI as a coupling agent showed the highest titers and affinities enabling measurement of LH RH levels lower than 1 pg/tube. The specificity of the antisera, particularly toward 13 fragments of the molecule, were similar irrespective of the immunogen used.

Animals↗
PubMed1976

[Production of antihaptene antisera. Comparison of methods of immunisation (author's transl)].

Five immunogenic substances were injected to rabbits. The method of immunisation by the intradermal route at 30 to 50 different points, with low doses of immunogen, without booster, does not seem to lead to production of antiserum of great specificity. The choice of the dose of immunogen to be injected is difficult, owing to our ignorance of the immunogenic dose for a given immunogen. The method of immunisation by the subcutaneous route with high doses followed by a booster, leads to more constant results, comparable to those obtained by the first method to which one may associate boosters by the subcutaneous route. The titers, affinities and specificities of the antisera thus produced are sufficient for the development of radio-immunoassay. The characteristics of the antisera obtained, related to the quality of the immunogen, do not seem to depend on the method of immunisation.

Angiotensin II↗
PubMed1976

[Radioimmunoassay of tetrahydroaldosterone in urine].

A radioimmunoassay for urinary tetrahydroaldosterone is described. An antiserum, elicited by a 3-carboxy-methyloxime 18-21 aldosterone diacetate conjugated to bovine serumalbumine, with which tetrahydroaldosterone cross reacts, is used. The method is specific, sensitive (10 pg/tube), accurate, reproducible (7%), thus allowing sufficient reliability for clinical applications. In 19 normal adults subjects under unrestricted sodium intake, the urinary tetrahydroaldosterone averaged 59,8+/-29,4 mug/24 h.

Adult↗
PubMed1976

[5 minutes incubation for the radioimmunologic determination of somatomammotrophic chorionic hormone].

A radioimmunoassay procedure for human chorionic somatomammotrophin in indiluted plasma has been developed in which the incubation lasts only five minutes rather than one half hour to four days in the previously described methods. The values obtained had excellent correlation (p less than or equal to 0,001) with those obtained by a two-hours technique. The intra-assay and inter-assay coefficients of variation are respectively 7 percent and 11 percent. Twenty women at term showed human chorionic somatomammotrophin values of 7.5 +/- 2.5 mug/ml plasma (mean +/- SD).

Female↗
PubMed1975

Circannual and circadian rhythms in plasma testosterone in five healthy young Parisian males.

Every other month, during the course of 14 months, the circadian rhythm in plasma testosterone (among others variables) of 5 healthy males (26 to 32 years) was documented. Subjects' synchronization: light-on at 7 +/- 1 h; light-off at 23 +/- 1.5 h during the whole year. Blood sampling 4-hourly, at fixed times during 28 h, for radioimmunological testosterone determinations. The cosinor method was used for statistical analyses. An annual rhythm in the testosterone 24-h mean is detected (P less than 0.025) with a peak in October (from 16 July to 26 December, with 95% confidence limits). Annual mean (+/-1 se)= 759 (+/- 26) ng/100 ml. Peaktrough difference=197 ng/100 ml (from 106 to 290 with 95% confidence limits). The timing of the testosterone circadian peak, in the 24-h-scales also varies during the course of the year. The peak is found around 8 h in May and around 14 h in November, this phase-shift being statistically significant (P less than 0.05).

Adult↗
PubMedDate not supplied

Opposite changes in dopamine metabolites and met-enkephalin levels in the ventricular CSF of patients subjected to thalamic electrical stimulation.

High-frequency electrical stimulations of thalamic nuclei are currently used for the suppression of parkinsonian or essential tremor and for the relief of some types of intractable pain in man. However, the mechanisms by which such stimulations exert their therapeutic effects are essentially unknown. Attempts were made to provide some insight into these mechanisms by measuring the levels of the dopamine metabolites homovanillic acid (HVA) and 3,4-dihydroxyphenylacetic acid (DOPAC), the serotonin metabolite 5-hydroxyindoleacetic acid (5-HIAA) and met-enkephalin-like immunoreactivity in ventricular cerebrospinal fluid (CSF) of patients with Parkinson's disease (PD) or multiple sclerosis (MS) after a 30-minute therapeutic electrical stimulation of the ventralis intermedius nucleus of the thalamus. In nonstimulated control patients, the levels of these compounds did not significantly differ in two CSF samples taken 30 minutes apart. In stimulated patients, a decrease in dopamine metabolite levels associated with a relative increase in met-enkephalin-like immunoreactivity were observed in the CSF sample taken after the 30-minute stimulation as compared to the sample taken immediately before the stimulation. In contrast, the levels of 5-HIAA remained unaffected by the stimulation. These data confirmed the existence of negative interactions between dopaminergic and enkephalinergic systems in man similar to those previously demonstrated in rats. In addition, they suggest that alterations in dopaminergic or enkephalinergic neurotransmission might be involved in the therapeutic action of thalamic electrical stimulation in patients with parkinsonian symptoms and other patients.

Adult↗