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Biomedical subjects

F Bricout

Publications and source records attributed to F Bricout.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

DNA hybridization for detection of human cytomegalovirus in bronchoalveolar lavage and pharynx biopsy.

A simplified hybridization procedure was used for detection of cytomegalovirus (CMV) in human specimens. The probe was a 32P-labelled cloned DNA fragment of CMV strain AD169. This probe did not hybridize to DNA from uninfected cells or other herpesviruses (herpes simplex virus, Epstein-Barr virus). Specific hybridization was obtained with unselected bronchoalveolar lavage specimens, but the sensitivity of the test (33%) was lower than that of culture (80%) and immunofluorescence (60%) assays which are routinely performed in our laboratory. The detection procedure was also carried out with pharynx biopsy specimens which had been kept frozen at -70 degrees C. CMV DNA was detected in 14% of tumour specimens and only in 1.7% of control specimens (p less than 0.05). The indications of DNA hybridization for CMV diagnosis are discussed.

Bronchoalveolar Lavage Fluid↗
PubMedDate not supplied

Reactogenicity and immunogenicity of rotavirus WC3 vaccine in 5-12 month old infants.

Rotavirus is a major cause of acute gastroenteritis in infants worldwide and there is need for an effective vaccine. Rotavirus Wistar calf 3 (WC3) is a strain of bovine origin attenuated by 12 passages in cell culture. A lyophilized candidate vaccine containing 1 x 10(7) PFU of WC3 has been developed. An oral dose was given to 25 French infants 5-12-months old (mean age 8.6 months). No diarrhoea was observed within 2 weeks after vaccination. Unexplained vomiting was reported once and isolated fever greater than 37.8 degrees C was reported 3 times during the first week. One month later, a neutralizing antibody response to serotypes tested was shown in 88% of cases, with heterotypic responses to human serotype 3 (SA11 strain) in 72% and to type 1 (WA strain) in 48%. The percentage of immune response was similar whether the infant had antibody prior to immunization or not, but a booster effect was observed in children who had pre-immunization rotavirus antibodies. Considering these promising results, efficacy trials are in the planning in different parts of the world.

Antibodies, Viral↗
PubMedDate not supplied

BK virus (BKV) detection in urine specimens of immunocompromised patients: comparison between the DNA-DNA hybridization assay, the immunofluorescence test and the dot enzyme immunoassay.

Three methods used for the detection of BK virus in urine specimens, the indirect immunofluorescence test, the dot enzyme immunoassay and the DNA-DNA hybridization assay, were compared by testing specimens from 49 immunocompromised patients. All three assays were effective in detecting BK virus. The technical advantage of each of them was discussed. The immunofluorescence test was found to be the simplest one to perform; the DNA-DNA hybridization assay displayed exquisite sensitivity; and the easy reading of the dot enzyme immunoassay did not require the specialized training inherent to immunofluorescence assays. The dot enzyme immunoassay might therefore be the most practical method for screening urine specimens of immunocompromised patients, especially when the sediment is poor in cells. Conversely, the indirect immunofluorescence test might be the method of choice for checking patients with haemorragic cystitis whose urine samples usually contain large amounts of cells.

BK Virus↗