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Biomedical subjects

F Bricout

Publications and source records attributed to F Bricout.

At least 37 records · Page 2Linked to original sources
PubMed1986

Simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus.

A simplified and reliable enzyme-linked immunosorbent assay (ELISA) was applied to the detection of serum antibodies against respiratory syncytial virus (RSV). RSV-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees C. The titers of reference sera were determined by endpoint dilution. A linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, P less than 0.001). Measurement of RSV antibodies was done by using a single serum dilution (1:1,000) in conjunction with a standard curve. A strong correlation was found between complement fixation and ELISA results (r = 0.89, P less than 0.001). In addition, the ELISA method exhibited higher titers and a greater sensitivity than did complement fixation, although the applicability of the assay is limited with positive serum samples of low titer.

Animals↗
PubMed1986

Serological characterization of human reassortant rotaviruses.

We analyzed the serological properties of two human wild-type cell culture-adapted rotaviruses (strains 308 and 46) and of 308 X 46 reassortants which were previously obtained and genetically characterized. Strain 308, exhibiting a so-called long RNA pattern, was found to belong to human rotavirus subgroup II, serotype 1, whereas strain 46, exhibiting a so-called short RNA pattern, represented subgroup I, serotype 2. Among the 308 X 46 reassortants we analyzed, two belonged to subgroup II, serotype 1, and exhibited short RNA patterns. This showed that the correlation observed between human subgroups I and II rotaviruses and the short and long electrophoretic patterns is not supported by any molecular basis (i.e., gene segment 10 or 11 was not involved in the subgroup specificity).

Antibodies, Monoclonal↗
PubMed1985

Epidemiology of human rotaviruses in a maternity unit as studied by electrophoresis of genomic RNA.

An epidemiological survey of Rotavirus infections in a maternity unit was carried out between May 1983 and August 1984. Rotaviruses have been detected throughout the survey in 184 of 528 fecal samples obtained from 3 and 6 day-old newborns. Rotavirus excretion could be detected neither from the mothers nor from the medical attendants. Rotavirus RNA was analyzed by polyacrylamide gel electrophoresis. All the strains isolated exhibited the same electropherotype. This result contrasts with the genomic variability amongst Rotavirus strains usually observed in pediatric wards, and is in agreement with some other studies conducted in maternity wards; in these locations Rotavirus infection seems to have a special epidemic status as compared with other communities.

Adult↗
PubMed1985

Monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions.

We developed five monoclonal antibodies against respiratory syncytial virus. Three of these (23A3, 12A4, and 18B2) were used in an indirect fluorescent antibody test, and the results were compared with those of a similar indirect fluorescent test with commercial anti-respiratory syncytial virus serum. The results obtained with antibody 18B2 and commercial anti-respiratory syncytial virus serum were identical, whereas with antibodies 23A3 and 12A4 the incidence of positive identifications was around 50%.

Antibodies, Monoclonal↗
PubMed1984

Characterization of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins.

Four classes of monoclonal antibody-producing cell lines have been obtained that detect human cytomegalovirus virion structural proteins. These antibodies react with (1) a major outer membrane virion glycoprotein(s) gp58-gp130, whose molecular weight varies between strains of cytomegalovirus, (2) a phosphoprotein, pp71, localized inside the virion membrane, (3) a phosphorylated nucleocapsid protein, pp155, and (4) a virion-associated phosphoprotein, pp29. Polyclonal immune human sera react with a large number of virion proteins including those detected by these monoclonal antibodies. These monoclonal antibodies were employed in a radioimmune assay to detect low levels (6 X 10(3) PFU/ml) of human cytomegalovirus in solution and human urine. These antibodies were also employed in a fluorescent antibody format to identify cytomegalovirus-infected cells obtained from human urine and nasopharyngeal aspirates. These reagents provide useful tools for studying the molecular biology of virus replication, for diagnosing cytomegalovirus infections, and for studying virus latency and activation.

Animals↗
PubMed1984

Study of genetic reassortment between two human rotaviruses.

Eight different human rotavirus strains were adapted to replicate in cell culture. In vitro, rotavirus genetic reassortment has been performed between two virus strains, one with a long pattern of segmented genomic RNAs and the other with a short pattern in the electrophoretic mobility of genomic RNAs. This genetic reassortment was detectable despite the lack of selective markers in the parental strains. Moreover, some of the reassorted virus exhibited mixed RNA patterns containing extra genomic RNAs resembling those found in clinical specimens. These viruses with mixed RNA band patterns could segregate their supplementary segments after successive passages in cell culture. Finally it has been shown that classification of genomic segments in long and short electropherotypes should not only be based on their relative size but also by their ability to reassort with the genetically equivalent segment.

Cell Line↗
PubMed1984

Survey of human rotavirus propagation as studied by electrophoresis of genomic RNA.

Rotavirus RNA isolated from three different environmental sources during the winter of 1981-1982 was analyzed by polyacrylamide gel electrophoresis. Our results demonstrate the presence of novel RNA patterns, the coexistence of multiple electrophoretic patterns in rotavirus isolated from samples from a given ward, and that spread of a given electropherotype is limited.

Child↗
PubMed1983

Isolation of a human pararotavirus.

During a survey of 350 different isolates of human rotaviruses, a single isolate with unique properties was obtained. Unlike all rotaviruses tested this unique isolate failed to react in an ELISA test with antibody directed against the group-specific rotavirus antigen. The morphology of this unusual virus isolate was identical in electron micrographs with other rotaviruses and the virus also contained 11 double-stranded RNA segments characteristic of this virus group. The electrophoretic migration of these RNAs (electrophoretype) in polyacrylamide gels did not coincide with the typical pattern of distinct size classes observed with all human rotaviruses. The unique isolate thus had two distinct properties that distinguish it from human rotaviruses, lack of a group-specific antigen and an atypical electrophoretype of the RNA segments. Based upon this, the new human isolate is given the name pararotavirus to distinguish this new type of virus group. Porcine and chicken pararotaviruses have recently been described with similar properties.

Antigens, Viral↗
PubMed1983

[Detection of rubella-specific IgM by an immunoenzyme technic].

An enzyme-linked immunosorbent assay (ELISA) was compared to sucrose gradient centrifugation technique for rubella specific IgM detection of 114 sera. The 28 sera which were rubella specific IgM positive, were also positive by ELISA. Among the 86 sera which were rubella IgM negative by centrifugation, 6 were equivocal and 2 were rubella IgM positive by the ELISA test, without clinical or epidemiological connection. These false results make a necessity of confirming by an immunoglobulin separation technique the ELISA IgM positive sera.

Adult↗
PubMed1982

A one-year virological survey of acute intussusception in childhood.

A prospective study was carried out during 1 year in order to correlate intussusceptions in childhood with a viral infection. A virus was incriminated in 50% of the 64 patients in this survey. Adenovirus infections remain predominant as compared to rotavirus infections.

Adenoviridae Infections↗
PubMed1980

Control of BK virus antibodies in contacts of patients under chronic hemodialysis or after renal transplantation (by an enzyme linked immunosorbent assay).

Elisa technique for IgG humoral BK virus antibodies titration is not time consuming, not expensive and brings results in accordance with those obtained by HI. It was observed among adults attending patients in chronic hemodialysis and renal transplantation centers an antibody level similar to observed titers in control subjects.

Adult↗