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Biomedical subjects

E Weigl

Publications and source records attributed to E Weigl.

At least 37 records · Page 2Linked to original sources
PubMed1993

Biochemical aspects of immunomodulation by Propionibacterium acnes.

The present paper deals with the effect of indomethacin and/or Propionibacterium acnes (Pa) on the cytochrome P-450 content in liver microsomal fraction, free fatty acids and lipase concentration in serum with respect to the phagocytic activities of monocytomacrophage system, changes in weight of the spleen and liver, survival after LD100 of Klebsiella pneumoniae, and histology of the spleen and liver. The results obtained indicate that INDO acts as an inhibitor of cytochrome P-450 and at the same time stimulates functions of the monocytomacrophage system in all the parameters studied. Pa, a classical immunomodulator, inhibits cytochrome P-450. After the initial suppression phase (3rd day after administration), it stimulates significantly the activity of monocytomacrophage system and increases the amount of FFA in the serum. The combination of both preparations also inhibits the cytochrome P-450 concentration in the liver and stimulates functions of the monocytomacrophage system in all the studied parameters. However, no temporary suppression of phagocytic activities was observed on the 3rd day of the experiment, in contrast to the animals administered Pa alone. Serum FFA concentration increased significantly. The application of both preparations accelerated markedly the onset of proliferation and differentiation processes in lymphatic follicles of the spleen.

Adjuvants, Immunologic↗
PubMed1992

Genetics of dermatophytes.

By the end of 60s a team of scientists has started at Medical Faculty of Olomouc the research of micromycetes pathogenic for both the humans and animals. In this study, the appropriate results are summarized as concerned with the genetics of dermatophytes. Among these results, those significant in deeper assessing the biology of dermatophytes and etiopathogenesis of dermatophytoses have been selected. In our opinion, they may be of interest for the successors in the direction mentioned. Here are also formulated certain open problems and the applicative outputs are traced, for example, those concerning with vaccines. The approaches used, even unusual in the medical mycology from a traditional scope, are believed to prove the convenience of genetic methods when researching mycopathogens. We regret to miss at the edition of this publication the late founder of the team, RNDr. Nora Hejtmánková, CSc. which had the greatest contribution to the Chapters on the karyology and variability of dermatophytes as well as to the hybridization analysis of Trichophyton mentagrophytes complex.

Arthrodermataceae↗
PubMed1992

Lymphocyte proliferation and T-lymphocyte subsets during experimental immunomodulation of Balb/C mice by Olimunostim.

To objectivize possible immunomodulatory effects of polybacterial lysate Olimunostim (P. acnes, K. pneumoniae, S. aureus), splenic lymphocyte proliferation and T-lymphocyte subsets were followed-up in Balb/c mice administered perorally the lysate or saline (controls). The enhanced spontaneous lymphocyte proliferation observed at the beginning of Olimunostim application preceded a gradual increase of lymphocyte reactivity to T-mitogens reaching the maximum five days after the administration of the last dose and returning back to the control levels ten days afterwards. This stimulation of lymphocyte proliferation was accompanied by Olimunostim induced increase of CD4+ splenic lymphocytes being most pronounced five days after the end of immunomodulation and later returning to the initial values. The last experiment revealed an enhanced response of the in vivo primed lymphocytes after the re-exposure to Olimunostim in vitro. It is concluded that mostly nonspecific activation mechanisms, plausibly also parallelly induced specific immunity, are involved in Olimunostim modulatory effects on the cellular immune response.

Adjuvants, Immunologic↗
PubMed1992

Study of modulatory effects of Olimunostim on cellular immunity in healthy humans.

To objectivize potential modulatory effects of polybacterial lysate Olimunostim on cellular immunity, the T-lymphocyte subpopulations and lymphocyte reactivity to mitogens were followed up in 10 healthy volunteers subjected to Olimunostim application and parallelly in 6 healthy individuals receiving placebo. The preparation, which was applied perorally in seven consecutive daily doses, had no effects on T-lymphocytes and their subpopulations and exerted only a limited influence on lymphocyte reactivity to mitogens. Both the moderate suppression of proliferative response to Con A and PWM, observed after the application of the three doses of Olimunostim, and the insignificantly enhanced response to PHA and Con A, revealed shortly after the last dose was given, were only of transient character and in comparison with the control group were not proved to be statistically significant. To explain the discordance of these results with the recently reported modulatory effects of Olimunostim on murine lymphocytes it is suggested, that the performed tests using the peripheral blood lymphocytes cannot mirror the processes developing in the diseased tissues. With respect to this presumption and also to the observations of enhanced immunomodulatory effect of Olimunostim on terrain altered by infection, it is proposed to investigate cellular immune parameters in specific disease sites of Olimunostim treated patients suffering from recurrent infections.

Adjuvants, Immunologic↗
PubMed1991

Values of selected immunological parameters before and after speleotherapy.

Changes of the selected immunological parameters before and after the speleotherapy are studied. It seems, that CIK and Complement parameters can be effected during the treatment in caves while Ig and LSZ parameters did not show any systematic changes. Details are available in the paper.

Antigen-Antibody Complex↗
PubMed1989

Cell wall of Candida albicans as a factor of pathogenicity.

The method of chemiluminiscence monitoring of oxygenous radicals was used for evaluation of human polymorphonuclear leukocytes activated by (1) intact cells of Candida albicans, (2) polysaccharide fraction of C. albicans cell walls. The results indicate that polysaccharide fraction of the cell wall activates the metabolic burst of PMN leukocytes as intact yeast cells and releases oxygenous radicals that play an important role in the origin of inflammatory response. The findings obtained allow to characterize polysaccharide fraction of cell walls as a significant pathogenic feature of C. albicans.

Candida albicans↗