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Biomedical subjects

E Teuscher

Publications and source records attributed to E Teuscher.

At least 37 records · Page 2Linked to original sources
PubMed1989

[Immunosuppressive and cytostatic effectiveness of the poplar mushroom Tricholoma populinum LANGE].

Dry powders or extracts of the fruit bodies of Tricholoma populinum given per as or injected intraperitoneally respectively caused a suppression of the number of plaque forming cells of mice as shown by the hemolysis-plaque-assay. Extracts of the fungi inhibited above that the incorporation of 3H-thymidine into mitogen-stimulated human lymphocytes as observed in the lymphocyte transformation test and retarded the proliferation of cultivated bovine aortic endothelial cells.

Animals↗
PubMed1989

[Immunosuppressive and virostatic actions of sterones from Petunia hybrida Vilm].

10 Ergostan-3-on derivatives presumably in the majority ergosta-1,4-dien-3-on derivatives could be isolated from Petunia hybrida VILM. Two were identified as the known compounds petuniasterone A and petuniasterone-C-22-O-acetate. The structures of two other compounds named petuniasterone-D-diacetate and 30-hydroxypetuniasterone A could be elucidated. The molecular formulas of the remaining substances were ascertained by mass spectroscopy. The petuniasterones inhibited the proliferation of stimulated human lymphocytes in non-cytotoxic concentrations, possessing therefore possibly immunosuppressive effects. Extracts of the plant and fractions containing a synergistically effective blue fluorescing substance retarded the multiplication speed of influenza viruses; pure petuniasterones were ineffectiv under our conditions in this regard.

Antiviral Agents↗
PubMed1988

Diminished adhesion of endothelial aortic cells on fibronectin and collagen layers after nonenzymatic glycation.

Adhesion of bovine endothelial cells on fibronectin and collagen before and after nonenzymatic glycation in vitro has been studied. Nonenzymatic glycation of these proteins reduced their ability to bind endothelial cells. Furthermore, nonenzymatically glycated fibronectin failed to bind to normal and nonenzymatically glycated gelatin and to fibrin. So gelatin and fibrin Sepharoses can be used to separate highly glycated fibronectins from fibronectins with a low degree of nonenzymatic glucose substitution. Sodium dodecylsulfate polyacrylamide gel electrophoresis did not demonstrate a covalent cross-link between nonenzymatically glycated fibronectins. These results present further evidences for the role of nonenzymatic glycation of proteins in the development of vascular complications in long-term diabetes and of atherosclerosis.

Animals↗
PubMed1988

Inhibition of cell migrations by the linoleic acid oxygenation product 9S-hydroxy 10E,12Z octadecadienoic acid (9-HODE).

The linoleic acid metabolite 9S-HODE was prepared by means of tomato fruit lipoxygenase and purified by high-performance liquid chromatography (HPLC) to a high steric purity as judged by chiral-phase HPLC. 9S-HODE caused in the concentration range between 0.01 and 1 microM a strong dose-dependent inhibition of the migration of both cultured porcine aortic endothelial cells and of phytohaemagglutinin-stimulated human mononuclear cells. The effect was not observed with another polyenoic fatty acid metabolite, 15S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-HPETE). The results are discussed in the light of other biological actions of 9-HODE recently described.

Animals↗
PubMed1988

[Cultivated aortic cells--demonstration of muscarinic receptors and reaction behavior in the presence of acetylcholine].

Using the muscarinic ligand 3H-quinuclidinyl benzilate the presence of muscarinic receptors on cells isolated from aortas of newborn rats and cultivated for 6 d could be shown. The number of receptors per cell was estimated with 17,400 on cells of confluent and with 87,100 on cells of nonconfluent cultures. Incubation of cells with increasing concentrations of acetylcholine in the range of 10(-13)-10(-7) mol.l-1 resulted in the reaction of growing numbers of cells following the all-or-nothing-law. Physiological concentrations of acetylcholine lead to contractions, showing that dilatations of blood vessels after application of acetylcholine in situ are caused by reaction products of endothelial cells, being absent in the cultures.

Acetylcholine↗
PubMed1987

Properties of in vitro nonenzymatically glycated plasma fibrinogens.

Nonenzymatic glycation of fibrinogen is species independent and depends only on the glucose concentration in the incubation mixture under selected in vitro conditions. An increased fibrin monomer aggregation in the presence of Ca2+ ions and a decreased proteolytic susceptibility of nonenzymatically glycated fibrinogens may favour the development of thrombophilic states. Blocking of lysine residues as well as restricted conformational changes induced by glucose attachment may be responsible for these effects. Fibrin stabilization by factor XIII is not impaired by nonenzymatic glycation of fibrinogen. Attachment of aortic endothelial cells to fibrin films from glycated fibrinogens is diminished. This phenomenon may be the result of blocked plasminogen activator binding sites in fibrin by nonenzymatic glycation. These effects may contribute in vivo to the accumulation of fibrin in those tissues most frequently affected by diabetic complications.

Animals↗
PubMed1987

[Potential antiangiogenic substances from alpha,alpha'-bis(amidinobenzyl)cycloalkanone derivatives and alpha-(arylsulfonylamino)-omega-phenylcarboxylic acid-4-amidinoanilide].

Assuming that the immigration of endothelial cells from existing blood vessels into tissues to be vascularized is the first step of neoangiogenesis, we examined the potential antiangiogenic activity of some synthetic inhibitors of serine proteinases on the migratory rate of isolated endothelial cell using Boyden technique. The 4-amidinoanilide of 2-tosylamino-4-phenylbutyric acid was shown to be the most effective inhibitor. Its effect is probably caused by inhibition of the plasminogen activators of tissue type produced by the cells.

4-Aminobenzoic Acid↗
PubMed1987

Interaction of drugs and the adenosine-uptake system of endothelial cells may influence the regulation of the heart by adenosine.

The uptake of adenosine by endothelial cells may be an important step in the metabolic regulation of the heart blood flow by adenosine. Cultivated endothelial cells served as a model for investigations of the inhibitory activity of vasoactive drugs on adenosine uptake by vascular endothelium. Dipyridamole, papaverine, prazosine, and nifedipine inhibited the adenosine uptake. This process depends also on energy metabolism of the endothelial cells.

Adenosine↗
PubMed1987

[Do endothelial cells participate in the biotransformation of cyclophosphamide?].

Investigations with cyclophosphamide were done to answer the question of participation of the endothelium in biotransformation of xenobiotics. Cyclophosphamide inhibits the proliferation of cultivated endothelial cells only in high concentrations. Also after induction of the cells by phenobarbital or 2,5-diphenoxazole no increased toxicity of cyclophosphamide was observed. Though the endothelium disposes of cytochrome-P-450-dependent aryl-4-monooxygenases it does not take part in biotransformation of cyclophosphamide apparently. Further possibilities of biotransformation by endothelial cells will be discussed.

Animals↗
PubMed1986

[Detection of muscarinic receptors on cultivated endothelial cells].

In order to contribute to the verification of the hypothesis of Furchgott et al. that the effect of acetylcholine on blood vessels is mediated by factors produced by endothelial cells, the authors tried to provide evidence for the existence of muscarinic receptors on these cells. Using cultivated cells of the endothelium of calve aortas, a specific saturable binding of the potent muscarinic antagonist 3H-Quinuclidinyl benzilate and therewith the existence of muscarinic receptors on the cells could be demonstrated. Long time cultivation leads to the disappearance of the receptors.

Animals↗
PubMed1986

[Establishment and characterization of the pig aortic endothelial cell line BSEz-3 in a serum-reduced medium].

The cell line BSEz-3 was established in a serum-reduced medium MEMPAS. The cells were isolated and cultivated under the same conditions as the calf aortic endothelial cell line BKEz-7. With regard to cell isolation and cell density in the stationary phase, BSEz-3-cells show characteristic differences from BKEz-7-cells. For the isolation of BSEz-3-cells an enzymatic-mechanical method was more successful than a mechanical technique only. The low saturation density in the stationary phase with an average number of 50,000 cells/cm2 of glass surface area is considered as a strong contact inhibition of cell proliferation. As a cell type specific marker the BSEz-3-cells contain factor-VIII-antigen and possess angiotensin-converting enzyme activity. For a practical use of the BSEz-3-cells in drug research we give recommandations regarding cell inoculum density for subcultivation and cryo-conservation in ampoules.

Animals↗