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Biomedical subjects

E Teuscher

Publications and source records attributed to E Teuscher.

At least 19 recordsLinked to original sources
PubMed2002

Effect of an orally applied herbal immunomodulator on cytokine induction and antibody response in normal and immunosuppressed mice.

The influence of the oral administration of a herbal immunomodulator, consisting of an aqueous-ethanolic extract of the mixed herbal drugs Thujae summitates, Baptisiae tinctoriae radix, Echinaceae purpureae radix and Echinaceae pallidae radix, on cytokine induction and antibody response against sheep red blood cells was investigated in mice. The treatment of the animals with the extract caused no enhancement of the cytokine titers in the serum. Spleen cells isolated from the treated mice, however, produced higher amounts of IL-2, IFNgamma and GM-CSF ex vivo in comparison to spleen cells isolated from control animals, especially after additional stimulation by lipopolysaccharides or concanavalin A. The application of the extract also triggered the production of IL-1 and TNFalpha by peritoneal macrophages ex vivo. The influence of the herbal extract on the antibody response was examined by the plaque forming cell assay. The administration of the extract caused a significant enhancement of the antibody response against sheep red blood cells, inducing an increase in the numbers of splenic plaque forming cells and the titers of specific antibodies in the sera of the treated animals. In mice, immunosuppressed by old age or additional treatment with hydrocortisone, the therapy with the extract resulted in a normalization of the antibody response against sheep red blood cells.

Adjuvants, Immunologic↗
PubMed1996

Cicutoxin from Cicuta virosa--a new and potent potassium channel blocker in T lymphocytes.

The effect of cicutoxin, the poisonous principle of the genus Cicuta, on K+ currents of activated T lymphocytes was investigated using the patch clamp technique. Cicutoxin produced a dose-dependent [5 x 10(-6) to 7 x 10(-5) mol/l] and completely reversible block of K+ currents with an EC50 of 1.8 x 10(-5) mol/l. A maximum block of 71% was achieved with cicutoxin at a concentration of 7 x 10(-5) mol/l. Since previous studies have shown that T lymphocyte proliferation is associated with K+ currents, the effect of cicutoxin on T lymphocyte proliferation was studied by means of 3H-thymidine uptake assays. At noncytotoxic concentrations [10(-7) to 5 x 10(-5) mol/l] cicutoxin reduced the 3H-thymidine incorporation dose-dependently. In conclusion, cicutoxin is a potent K+ current blocker which inhibits K+ channel-dependent proliferation of naive and memory T lymphocytes.

Alkynes↗
PubMed1996

Effects of some components of the essential oil of chamomile, Chamomilla recutita, on histamine release from rat mast cells.

The influence of three compounds of the essential oil of Chamomilla recutita (L.) Rauschert on the protamine sulphate-provoked degranulation of mast cells from Lewis-1a rats was investigated. The effect was determined by measuring histamine liberation fluorometrically. Chamazulene and (-)-alpha-bisabolol had no distinct effects. The en-yne dicycloether partly inhibited the degranulation in concentrations above 10(-4) M.

Animals↗
PubMed1992

[The effect of cholesterol on membrane potentials, proliferation and migration of cultivated endothelial cells].

The influence of cholesterol added as cholesterol containing liposomes [sphingomyelin/cholesterol (1: 1; mol/mol)] or as cholesterol suspensions on cultured bovine aortic endothelial cells was investigated. 10(-6) mol/l cholesterol enhanced membrane potential, 10(-5) mol/l caused depolarisation. The proliferation of the cells was dependent on the concentration influenced in opposite directions too. The proliferation was stimulated by 10(-6) mol/l cholesterol and inhibited by 10(-5) mol/l. The migratory rate of the cells was increased by 10(-6) mol/l and 10(-5) mol/l cholesterol. Our results suggest that exogenous cholesterol is integrated in membranes of the endothelial cells and causes in this way changes of membrane potential, proliferation rate and migratory activity.

Animals↗
PubMed1991

Stimulation of endothelial cell migration by thrombin.

The blood proteinase thrombin was shown to stimulate the migration of porcine aortic endothelial cells in vitro. This effect seems to be dependent on the receptor binding of thrombin to the target cell. Prostaglandins could mediate endothelial cell migration by acting as "second messengers".

Animals↗
PubMed1991

[Investigations of the immunomodulatory effect of cyanobacterial extracts].

Resulting from the knowledge that cyanobacteria (blue-green algae) are able to produce pharmacologically active substances the aqueous extracts from several cyanobacteria species and strains (Microcystis aeruginosa, Synechocystis aquatilis, Oscillatoria redekei, Anabaena flos-aque, Aphanizomenon flos-aquae, Oscillatoria rubescens, Oscillatoria tenuis) were tested for their immunomodulating activity. Extracts from Oscillatoria redekei 051, Oscillatoria tenuis 01 and Synechocystis aquatilis 428 caused an immunosuppression. They inhibited not only the incorporation of 3H-thymidine into mitogen stimulated lymphocytes but reduced also the number of plaque-forming cells of mice as shown by hemolysis-plaque-assay. Only extracts from Oscillatoria redekei 051 did not show any cytotoxic effects in lymphocyte cytotoxic test. This may be an evidence for a specific action on the proliferation of lymphocytes.

Antibody Formation↗
PubMed1989

[The ectophosphatase activity of cultured endothelial cells of calf aorta and the effect of drugs on ecto-ATPase].

Cultivated endothelial cells of calf aorta (line BKEz-7) possess an effective ectophosphatase system (enzyme activities: ATPase 38.0 +/- 10.2; ADPase 9.2 +/- 4.2; 5'-nucleotidase 4.1 +/- 2.6 fmol/cell.min). Drugs with central depressive activity such as promazine, chlorpromazine, and meprobamate inhibit the activity of the ecto-ATPase. A possible connection between the inhibitory activity on the ecto-ATPase and their central depressive effects is discussed.

5'-Nucleotidase↗
PubMed1989

[The effect of alkyl-lysophospholipids and membrane potentials, proliferation and migration of isolated calf aorta endothelial cells].

Cancerostatical active synthetic alkyl-lysophospholipids were examined with regard to their effects on membrane potential, proliferation and migration of isolated endothelial cells. In sublytic concentrations all alkyl-lysophospholipids tested caused a hyperpolarization of the membrane of endothelial cells. The effect of alkyl-lysophospholipids on proliferation of endothelial cells was dependent on the serum supplement. The migration of endothelial cells was strongly inhibited by 1-O-octadecyl-2-O-methyl-glycero-phosphocholine. Possible mechanisms of action are discussed.

Animals↗