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Biomedical subjects

E Takeuchi

Publications and source records attributed to E Takeuchi.

At least 217 records · Page 12Linked to original sources
PubMed1982

[Concentration of sulbenicillin in human serum and myocardial tissue].

The concentration of sulbenicillin in the serum and myocardial tissue of 13 patients were determined at cardiac surgery. About 100 mg/kg of sulbenicillin were administered intravenously for 60 minutes. Right auricle resected at the vena caval cannuration was examined for myocardial tissue. The serum concentration was also examined every 30 minutes after injection, 30 minutes, 60 minutes, 90 minutes, 120 minutes and 150 minutes. Mean values of serum concentration were 213.6 micrograms/ml, 432.5 micrograms/ml, 181.6 micrograms/ml, 236.3 micrograms/ml and 101 micrograms/ml, respectively. The values determined in myocardial tissue were 146.5 micrograms/g (60 minutes), 39.2 micrograms/g (90 minutes), 66.0 micrograms/g (120 minutes) and 23.5 micrograms/g (150 minutes). The mean value of concentration ratio of sulbenicillin in myocardial tissue was 0.23 of serum concentration. The high myocardial tissue levels of sulbenicillin suggests that its use at cardiovascular surgery would protect the myocardial tissue from the bacterial infections.

Adolescent↗
PubMed1981

Either high-mannose-type or hybrid-type oligosaccharide is linked to the same asparagine residue in ovalbumin.

After pepsin digestion, all of the carbohydrates in ovalbumin were recovered in two glycopeptides, Glu-Glu-Lys-Tyr-Asn(CHO)-Leu-Thr-Ser-Val and Glu-Gln-Lys-Tyr-Asn(CHO)-Leu-Thr-Ser-Val. Almond glycopeptidase released quantitatively oligosaccharides from the glycopeptides. The products from both glycopeptides contained both the high-mannose-type oligosaccharides and the hybrid-type oligosaccharides in the same ratio. Thus, either the high-mannose-type or the hybrid-type oligosaccharide is attached to the unique asparagine residue in the ovalbumin molecule.

Amidohydrolases↗
PubMed1981

Essential Role of T cells in the postexposure prophylaxis of rabies in mice.

Athymic nude mice injected intramuscularly with a street stain of rabies virus were not protected against rabies by postexposure administration of beta-propiolactone-inactivated rabies vaccine. In contrast, their normal littermates were completely protected from death by the same vaccination regimens. Nude mice did not produce IgG antibody as a result of the vaccine during the test period of 15 days, whereas normal littermates produced IgG antibody from day 5 after vaccination. However, passive immunization with antirabies hyperimmune mouse ascites showed that antibody was completely ineffective in protecting either nude mice or their normal littermates against rabies when given later than 2 days after infection. No significant difference in the induction of circulating interferon by the vaccination was noted in these mice. Passive transfer of immune spleen cells to nude mice immediately after infection resulted in 30 to 37.5% protection of the mice. Passively transferred spleen cells did not produce detectable amounts of neutralizing antibody in the recipient mice except on day 2 after the transfer, when a low level of antibody was detected. These observations demonstrate the essential role of T cells in the postexposure prophylaxis of rabies in mice. The mechanisms of the failure of postexposure vaccination in nude mice are discussed.

Animals↗
PubMed1981

Effects of corrective surgery on natural history of atrial septal defect of secundum type.

Two hundred and seventy-eight patients with atrial septal defect of secundum type were operated on surgical closure of the defect. The patients were divided into 5 groups according to age at surgery, and preoperative complaints and laboratory findings were analyzed to evaluate natural history. As year passed, incidence of complaints increased. Cardiothoracic ratio (CTR), systolic pressure of the right ventricle (PRVS), end-diastolic pressure of the right ventricle (RVEDP), mean pressure of the right atrium (PRAm), ratio of pulmonary to systemic systolic pressure (PPA/PS), right ventricular dimension (RVD) and left atrial dimension (LAD) increased significantly, while frontal axis of the QRS complex, RV1, SV1 +RV5 and LVD decreased. No chronological changes were seen in ratio of pulmonary to systemic blood flow (QPA/QS) and ratio of pulmonary to systemic vascular resistance (RPA/RS). The first and second decades of life were considered to be stable stages of the disease. Postoperatively, changes in the above-mentioned parameters were compared in each age group, and effects of surgical repair on natural history were evaluated. Of 265 survivors, 168 were followed-up for more than one year, the longest period being 22 years. CTR, frontal axis, RV1, PRVS, RVEDP, PPA/PS and RVD decreased significantly, while SV1 + RV5, RPA/RS and LVD increased. No changes were seen in LAD. Surgical effects appeared most significantly in the first decade and least in the fifth or more. From these findings it would be concluded that corrective surgery should be best carried out in the first decade, at latest in the second, though not to be contraindicated by age alone. Otherwise, postoperative improvement of parameters delays or hemodynamical abnormalities may persist regardless of considerable clinical improvement.

Adolescent↗
PubMed1980

A mouse model of the pathogenesis and postexposure prophylaxis of rabies.

A mouse model for the study of postexposure prophylaxis of rabies was established. Mice injected intramuscularly with a street strain of rabies virus were significantly protected from death by five daily 0.2-ml doses of inactivated rabies vaccine of chick embryo cell culture origin initiated immediately or 3 hr after infection. In these mice, a large amount of circulating interferon was induced as early as 1 hr after the first dose of vaccine and lasted until at least 12 hr but no such amount of interferon was induced by additional doses of vaccine. Serum antibody was first detected in the mice on day 6. It was noted that some of the surviving mice manifested an ataxia or paralysis of the legs. Increasing mortality rates were shown in mice treated with decreasing doses of the vaccine. Passive protection tests using concentrated IgG and IgM antibodies with equivalent neutralization titers showed that IgG antibody gave total protection when given 24 hr before the infection, while it was almost totally ineffective in reducing the mortality when given 2 days or more after infection. IgM antibody did not protect the mice even when given 24 hr before infection. These results suggest that interferon production is more important than antibody production in the initial stages of protection by postexposure vaccination. However, the mechanisms of postexposure prophylaxis in this model could not be explained only by the interferon produced by the vaccine and the possible contributions of additional mechanisms were suggested.

Animals↗
PubMed1979

[Effects of benzodiazepine derivatives on gamma-motor system in rats (author's transl)].

Effects of four benzodiazepine derivatives on gamma-activity were examined in the anesthetized rat. Diazepam and 5-(o-chlorophenyl)-1-methyl-7-nitro-1, 3-dihydro-2H-1, 4-benzodiazepin-2-one(ID-690) in a dose of 2.5 mg/kg (i.p.) showed depressant effects on gamma-activity. The depressant effects of 5.0 mg/kg (i.p.) lasted for more than 60 min. Nitrazepam had a slightly weaker effect than the above two drugs and the effect was evident after an administration of 5.0 mg/kg; the effect of 10 mg/kg(i.p.) lasted for more than 90 min. The effect of clonazepam was much weaker than effects of the other three drugs. A dose of 20 mg/kg(i.p.) was required to produce an obvious depressant action, and the effect appeared after a longer latency of about 20 min. ID 690 (5.0 mg/kg, i.p.) depressed the augmented response of the gamma-activity in response to pinna stimulation. The effect of diazepam on the augmented responses was observed with the same dose. In contrast, much higher doses of nitrazepam and clonazepam were required to induce an obvious depressant effect on the augmented gamma-activity. It is suggested that the difference in potency in depressing gamma-activity of these derivatives is one of the factors characterizing their pharmacological properties.

Animals↗