Search PubMed⌕ Search

Biomedical subjects

E Suchanek

Publications and source records attributed to E Suchanek.

At least 55 records · Page 3Linked to original sources
PubMedDate not supplied

[Validity of the direct immunofluorescent monoclonal antibody test in the diagnosis of chlamydial infection].

The sensitivity and specificity of direct fluorescent monoclonal antibody staining were compared with the isolation of the organisms in McCoy cells. For both diagnostic methods cervical smears were taken in 120 women. Of 120 women 25 (20.8%) were infertile, 60 (50.0%) healthy fertile women and 35 (29.8%) adolescents who came for control gynecological examination. C. trachomatis was isolated in 5/25 (20.0%) of infertile women, in 5/60 (8.3%) of healthy fertile women and in 4/35 (11.4%) adolescents. With the cell culture as the reference method, the antigen detection test had a sensitivity of 92.8%, a specificity of 98.1%, a positive predictive value of 86.7% and a negative predictive value of 99.1%.

Adolescent↗
PubMedDate not supplied

[Prenatal diagnosis of congenital adrenal hyperplasia due to 21-hydroxylase enzyme deficiency].

Prenatal diagnosis of the "classical" forms of congenital adrenal hyperplasia (CAH) which is a result of 21-hydroxylase (21-OH) deficiency either complete, with salt-wasting or incomplete without salt wasting, is performed in two ways: by measuring concentration of 17-hydroxyprogesterone (17-OHP) and androstendione (delta 4) in amniotic fluid and by HLA typing of fetal cells from amniotic fluid. Having ones own normal values is the basic condition for the safe prenatal diagnosis of CAH 21-OH deficiency by measuring steroid concentration in amniotic fluid. Normal concentrations of 17-OHP in amniotic fluid achieved by amniocentesis in 85 pregnant women from 16-23 gestation week have been measured, as well as concentrations of delta 4 in 66 pregnant women in the same period of gestation. It has been proved that there are no differences between the concentrations of delta 4 in amniotic fluid regarding the sex. As far as 17-OHP is concerned, the same was confirmed earlier. The results of 9 prenatal diagnosis in 8 families, having already one child with "classical" form of CAH with salt-wasting, have been presented. It was achieved by combination of two methods: by measuring concentration of 17-OHP and delta 4 in amniotic fluid and HLA typing of fetal cells from amniotic fluid. In 8 fetuses at risk the birth of healthy children was correctly predicted, which was confirmed after the birth in three cases by HLA typing and measuring concentration of 17-OHP and delta 4 and from the blood of newborn babies.(ABSTRACT TRUNCATED AT 250 WORDS)

Adrenal Hyperplasia, Congenital↗
PubMedDate not supplied

[Fertilization in vitro in the treatment of male infertility].

Thirty marital couples were included in an in vitro fertilization study of male infertility. Second-degree oligospermia (less than or equal to 5 X 10(6)/ml mobile sperms) was found in 3 couples, first-degree oligospermia (less than or equal to 12 X 10(6)/ml mobile sperms) in 21 couples, and asthenospermia (less than 20% of mobile sperms) in 6 couples. The semen for insemination was prepared by the standard procedure and the overlay technique. After the separation (removal) of seminal fluid and capacitation the sperms were found to be significantly more mobile. Ovarian cells obtained by laparoscopy were inseminated with 50,000 to 250,000 mobile sperms. The results were compared with those in a group of patients with normospermia (greater than or equal to 12 X 10(6)/ml mobile sperms). The number of fertilized ovarian cells proved to be significantly lower in patients with second-degree oligospermia (P less than 0.001). There was no difference in the rate of fertilization and ovarian cell division either in patients with first-degree oligospermia or asthenospermia or normospermia. Seven clinical pregnancies were recorded: 2 in the second-degree oligospermia group, 4 in the first-degree oligospermia group, and 1 in the asthenospermia group.

Female↗
PubMedDate not supplied

[Pregnancy after fertilization in vitro and embryo transfer].

The first 13 pregnancies established by in vitro fertilization and embryo transfer. A tubal factor was the main indication for in vitro fertilization (IVF) in 10 patients, whereas in three couples infertility was caused by the infertile sperm. The mean age and duration of infertility in female patients were 33.5 and 11.4 years, respectively. Multiple follicular growth was stimulated by either clomiphene citrate (100-150 mg during 5 days), clomiphene citrate + HMG + HCG, or HMG + HCG. Timing of ovulation was determined by assaying serum estradiol, progesterone and LH levels. The ultrasound monitoring of follicular growth was determined on a daily basis. Endogenous LH surge was observed in one patient, whereas in others 5000-10,000 IU HCG were needed to induce ovulation. All oocytes were recovered by laparoscopy. There were 3.8 oocytes per patient on an average. Fifty-six percent of oocytes were preovulatory. All oocytes were allowed to mature in vitro for 3 to 7 hours (mean 5.7 hours). The fertilization and cleavage rates were 76% and 72% respectively. Only one polyspermic fertilization was observed. Forty-five hours after the insemination the embryos between the 2-cell and 6-cell stages were reimplanted into the uterus. Using various ET cannulas, one to seven embryos per patient (2.7 embryos on the average) were replaced into the uterine cavity, 5 mm below the fundus. Only four embryo replacements were completely atraumatic. No medications were used for the luteal phase support. The corpus luteum function and early pregnancy were controlled by serial measurements of serum progesterone, estradiol and beta HCG. Three clinical miscarriages and full-term deliveries were recorded.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMedDate not supplied

[The effect of delayed insemination on fertilization in vitro and the development of human embryos].

Extracorporeal fertilization of 363 oocytes was applied in 148 patients with tubal infertility. The aspiration of the follicles was performed 36 to 38 hrs after the onset of the endogenous LH rise or 36 hrs after injection of hCG. Oocytes maturity was evaluated on the basis of the morphological appearance of the cumulus oophorus. The eggs were incubated in culture medium before insemination. The duration of delayed insemination had a marked effect on the proportion of fertilized oocytes and the development of embryos (X2 = 10.34, P = 0.05). After maturation of 1.5 to 3.5 hrs, minimal preovulatory oocytes were fertilized and developed to embryos. Maturation of 3.5 to 5.5 hrs increased the number of fertilized oocytes from 56% to 71.7% and the number of embryos from 50% to 55.1%. After 5.5 to 7.5 hrs of maturation significantly more fertilized oocytes (t = 2.4, P less than 0.02) and developed embryos (t = 2.41, P less than 0.02) were obtained. Preincubation of intermediary preovulatory or immature oocytes had no beneficial effect on the fertilization rate and the development of embryos.

Adult↗
PubMedDate not supplied

[Human beta chorionic gonadotropin in the early diagnosis of extrauterine pregnancy].

In 1979 and 1980, at the University Hospital Department of Gynecology and Obstetrics, 115 patients with extrauterine pregnancy were surgically treated. In 26 patients the value of the radioimmunologic determination of beta chorionic gonadotropin (HCG) in the serum was analysed. Along with it, also other clinicodiagnostic procedures (immunochemical test for pregnancy, culdocentesis, curettage) wire carried out. Not in a single case of extrauterine pregnancy in which the serum beta HCG level was determined, was the result falsely negative. The immunochemical test for pregnancy was falsely negative in 32.6% and the result of culdocentesis was falsely negative in 13.6% of the patients examined. In patients with negative culdocentesis laparoscopy was performed (10.4%), showing ectopic pregnancy. In the course of extrauterine pregnancy the elimination of beta HCG was significatly reduced in relation to its level in normal pregnancy. The concentration of beta HCG in the observed patients with extrauterine pregnancy ranged from 27 to 3924 IU/L. On the basis of a low beta HCG concentration in the serum it is possible to determine with much certainty that pregnancy is not quite normal. Of great help is the observation of changes in the elimination of this hormone over a short period of time. A timely diagnosis of extrauterine pregnancy improves prognosis of future fertility.

Adult↗
PubMedDate not supplied

[The effect of epidural analgesia in labour upon serum catecholamine levels].

The serum levels of catecholamines were determined in 26 healthy parturients. Epidural analgesia was given in 13 cases, while the remaining women were given classic parenteral analgesia and served as controls. The blood samples were taken before analgesia and after delivery and also from the clamped umbilical vein from the newborn after delivery. There was a statistically significant decrease of catecholamine levels in the epidural group and a slight increase in the control group. There were no differences among the newborns.

Adult↗