Search PubMed⌕ Search

Biomedical subjects

E Somogyi

Publications and source records attributed to E Somogyi.

At least 19 recordsLinked to original sources
PubMed1991

Dynamics of creatine kinase shuttle enzymes in the human heart.

Myocardial cytoplasmic creatine kinase subunits M and B, mitochondrial CK (CKMIT), and citrate synthase (CS) were determined in 10 locations of the normal human heart (n = 8) and in papillary muscles of patients operated on for mitral regurgitation (n = 6). Compared to atrial biopsies, septal and left ventricular biopsies showed higher activities for CS (P less than 0.0001), total CK (P less than 0.05) and CKMIT (P less than 0.0001). CKM was evenly distributed. CKB activity in the right septum and left ventricular locations were 0.5-1% of total CK and 4-5 times lower than those of the atria and the right ventricular free wall. Activities of CS, CKB and CKMIT in right septal biopsies did not differ from those in left ventricular locations. The activities of CS, total CK, and CKM in papillary muscle from patients operated on for mitral regurgitation did not differ from that of healthy papillary muscle. CKMIT was about 40% lower (P less than 0.02), whereas CKB was 15-20 times higher (P less than 0.0001) than in the healthy heart. In conclusion, adaptations within the creatine kinase system occur in the human heart in health and disease. Small amounts of CKB in the normal left ventricle, as opposed to the right ventricular free wall, might be related to differences in myocardial perfusion during the cardiac cycle. In disease, a decreased CKMIT and dramatically increased CKB may indicate a stressed intracellular energy transfer. CK enzyme activities in right septal biopsy specimens may be used as an indication of metabolic stress on the myocardium of the left ventricle.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1991

[Why should teeth, used for anchoring partial prostheses, also be used as their support?].

It is proved by means of static models that, as regards the load of the periodontium, the teeth employed for the dental supporting and anchoring of partial dentures are in a much more favourable situation than the teeth employed but for anchorage. Viz. while the resultant of the axially and laterally directed forces resulting from supporting and anchoring does not become eccentric, the direction of the but anchorage force points out of the structure and entails an unfavourable torque on the tooth.

Dental Abutments↗
PubMed1990

Immunofluorescence demonstration of digoxin in human myocardial tissue and isolated rat cardiac myocytes with monoclonal antibodies.

The aim of the present investigation was to identify the morphological correlates of digoxin binding sites in human heart muscle tissue and isolated viable rat heart myocytes. Cardiac glycoside linked to myocardial cells was demonstrated by monoclonal digoxin specific antibody and by FITC-conjugated anti-mouse immunoglobin serum. This versatile immunofluorescence method can be used in diagnostic and experimental studies of cardiac glycoside binding.

Animals↗
PubMed1990

Myoglobin content and citrate synthase activity in different parts of the normal human heart.

Myoglobin (Mb) content and citrate synthase (CS) activity were determined in myocardial samples from nine human brain-dead organ donors with normal hearts. Six regions of each heart were analyzed: right and left atria, right ventricle, left ventricular subepicardium, subendocardium, and anterior papillary muscle. The Mb content was similar, whereas the CS activity was higher in the left than in the right heart at both atrial and ventricular levels. Mb content and CS activity were higher in ventricles than in atria. The subendocardial layer and papillary muscle of the left ventricle had a higher Mb content than the subepicardial layer, whereas CS activity was similar in these three locations. The results suggested a closer relationship between CS activity (oxidative potential) and work load than between Mb content and work load. Mb content may, instead, be related to intramuscular oxygen tension (PO2) on the basis of a comparison between our Mb data and those of others on regional variations in myocardial PO2.

Adolescent↗
PubMed1990

Comparative morphological and biochemical investigation of healthy human myocardium.

In order to study healthy myocardium ultrastructural and biochemical investigations were performed on human heart in biopsies taken from different locations from brain dead organ donors. To study healthy myocardium and to recognize early signs of myocardial impairment, morphological and biochemical investigations were performed on human myocardial tissue in various conditions. The lactate dehydrogenase was investigated cytochemically in different part of the myocardium. The cytochemical reaction did not show difference. Lactate dehydrogenase and its isoenzyme activities were determined in different parts of the normal human myocardium. In conclusion LD isoenzyme dominates in all parts of the normal human myocardium. The activities of total LD, H subunit and LD1 increase from atria and auricular appendages via right ventricle to left ventricle. The activities of both H and M subunit are different in the different parts of the myocardium. Combining the morphologic and enzymatic evaluation of endomyocardial biopsy samples, it is possible to get closer to the understanding of different cardiac diseases and to increase the value of diagnostics.

Biopsy↗
PubMed1990

[Restoration of unilateral borderline edentulousness (Fábián-Fejérdy prosthetic class 2A-2B)].

A frequent case of unilateral row and edentulousness is when, on one side, all three molars are missing and, thus, the second promolar is the last tooth. To solve such edentulousness a removable denture or a combined fix plus removable plate denture is suggested. By employing the principles of the prosthetical classification (Fábián-Fejérdy) the justification of the distally free end bridge is proved.

Denture Design↗
PubMed1990

[The damaged environment. Heart and lung changes].

Author evaluates cardiac and pulmonary changes developed by environmental pollution, from the point of view of forensic expert. The myocardium and parenchyma are the target organs of multicasual damages. Attention is called to the role of forensic expert in prevention of environmental damages and in the evaluation of the effects.

Environmental Pollutants↗
PubMed1989

The asymmetry of 3H-imipramine binding may predict psychiatric illness.

The Bmax and Kd values for 3H-imipramine binding were measured in post-mortem human brains from drug-free selected psychiatric subject homicide victims (n = 15) and normal controls (n = 15). The two groups were comparable in age and gender. The number of imipramine binding sites (Bmax) in the frontal cortices of psychiatric subjects had significantly higher Bmax values in the left hemisphere than in the right hemisphere. Inversely, the number of imipramine binding sites (Bmax) in the frontal cortices of normal controls were significantly higher in the right brain than in the left brain. It was postulated that the inhibiting effect of central serotonin (5-HT) has weakened in psychiatric cases, therefore the change of presynaptic serotonergic activity might be associated with psychiatric illness in the left hemisphere of human brain.

Adolescent↗
PubMed1989

Lactate dehydrogenase and its isoenzyme activities in different parts of the normal human heart.

Activity and distribution of lactate dehydrogenase (LD) and its isoenzymes (LD1-5) were determined and both the heart (H) and muscle (M) subunit activities were calculated in myocardial samples from six brain dead human organ donors with normal hearts. Ten parts of each heart were analysed. LD1-3 were found to be the main LD isoenzymes. The LD1 activity predominated in all parts analysed. The activities of total LD, H subunit and LD1 increased from atria and auricular appendages via the right ventricle to the left ventricle. The H subunit activity varied more than twofold and the M subunit activity by only 20% between different locations of the heart. The left ventricular papillary muscle was found to have higher activities than other locations of both H and M subunits. It is concluded that the isoenzyme profile could be a metabolic adaptation to divergent work demand on the different heart chambers.

Adaptation, Physiological↗
PubMed1989

Immunofluorescence detection of digoxin with monoclonal digoxin specific antibody.

The present study primarily focuses on the analysis of digoxin binding of the heart muscle cells. The primary aim of the investigation was to demonstrate the cardiac glycoside morphologically. The direct immunofluorescence staining technique with digoxin specific monoclonal antibody or Fab fragments and FITC or Texas-Red conjugated antisera are useful for morphological demonstration of digoxin binding and localization in cardiac cells. With the immunofluorescence method, linkage can be observed on the sarcolemma membrane and on the wall of capillaries and arterioles in myocardial cells treated by cardiac glycoside. The specificity of reaction is provided by the negative reaction of cells, not treated by digoxin. Intensity of reaction depends on concentration. The photometric measuring of fluorescence enables the quantitative analysis of cardiac glycoside. It shows the sensitivity of the method in that cardiac glycoside linked to the cell membrane can be detected in the upper sphere of a therapeutic dose. Application of the immunofluorescence method is manifold and relatively simple, and this quick method can be used in diagnoses and in the study of cardiac glycoside receptors of cell membrane. On the basis of our own experiments it is possible to study the kinetics of digoxin linkage. The use of this method is demonstrated for investigation of single cell suspension and cryostat sections.

Animals↗
PubMed1989

Analysis of chromosomes in paternity cases.

Authors on the base of the material investigated in the Department of Forensic Medicine of Semmelweis Medical University discuss importance of anthropological investigations as a valuable, additional to serology test. In cases when neither blood-group tests nor anthropology provided evidence sufficient to formulate a conclusive opinion chromosomal studies are recommended. The surveying of 300 cases with the C-band/showed the thickening of the heterochromatic part of chromosomes 1, 9, 16, Y/and the occurrence of total and partial inversion. Examinations with Q-band have revealed the incidence of chromosomal polymorphism.

Chromosome Banding↗
PubMed1989

[Evaluation of distal free-end dentures and partial plate dentures based on their effect on the remaining dentition].

The data of 3778 examined persons were processed. The effect of distally free end bridges made for replacing terminal endentulousness on the remaining teeth was compared with the condition of the remaining teeth of patients untreated, provided with partial dentures as well as with combined fix + partial denture. It was ascertained that in cases of replacements by distally free end bridges deterioration of the remaining teeth is essentially less.

Denture, Partial, Fixed↗