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Biomedical subjects

E Seidler

Publications and source records attributed to E Seidler.

At least 55 records · Page 3Linked to original sources
PubMed1980

[1-methoxy-5-methylphenazinium-methylsulfate--a light stabile redox mediator in the histochemistry (author's transl)].

1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) was tested for use as redox mediator in histochemistry of dehydrogenases. Aqueous solutions of MPMS are absolute lightstable. Like PMS or Meldola Blue the succinate dehydrogenase reaction was increased by MPMS. In an non-enzymatic NADH-NBT-test the reaction rate of MPMS exceeds the rates of PMS or Meldola Blue.

Animals↗
PubMed1980

[On the birefringence of formazans (author's transl)].

The enzyme succinate dehydrogenase was demonstrated histochemically in organs of rats, especially in experimentally infarcted hearts. 88 tetrazolium salts were used as indicators, the resulting formazans were examined for birefringence. Formazans with cristalline shapes show birefringence, but formazans of amorphous character do not. In the atypic species of nitrogroup-substituted monoformazans it is possible to form an oriented association to cell structures in spite of an amorphous precipitation. Therefore, anomalous colours of polarization, and dichroism is observed. Relations are discussed between shape of formazan deposits, polarity of formazans, and the occurrence of optical anisotropism.

Animals↗
PubMed1979

The teaching of medical ethics in the Federal Republic of Germany.

Eduard Seidler sets his discussion of the teaching of medical ethics in the Federal Republic of Germany against an historical background. Immediately after the Second World War the freshness of the memory of the 'Nuremberg Medical Trials' influenced the way in which moral dilemmas were treated in Germany. At the present time no systematic instruction in medical ethics is provided in either undergraduate or postgraduate or continuing medical education. As a result of this, an inquiry was set up in 1977/78. Questionnaires were sent out with a view to collecting information on how subjects referring to medical ethics are treated during medical training. The inquiry showed that no special discipline can claim to be the only competent one for medical ethics, but that everyone has something to contribute. Dr Seidler concludes by stating that basic curricula related to the problems of medical ethics would have to be developed and should be carried on continuously within the training programme.

Curriculum↗
PubMed1979

[The mechanism of the tetrazolium reduction and the effect of phenazinmethosulfate (author's transl)].

Modern results of the literature form the basis for the presentation of a scheme which shows the reduction of tetrazolium salts and the action of phenazine methosulfate under histochemical conditions. The reduction of tetrazolium salts passes the radical state and it hapens principally on the niveau of the flavin proteins and the ubiquinone. The importance of the superoxide radical for the reduction of tetrazolium salts under aerobic conditions is emphasized.

Aerobiosis↗
PubMed1978

Determination of infarct size in rats with 86rubidium.

A quick method is presented which allows the determination of infarct sizes within hearts of experimental animals like rats. The method is based on the drop of 86Rb uptake in infarcted hearts, and has been compared with infarct sizes determined morphometrically. The standard deviation of this method is low (6.5%) because an internal standard in used (noninfarcted myocard - usually right ventricle tissue - has been used).

Animals↗
PubMed1978

[The qualification of different ditetrazolium salts as indicators in the oxido-reductase histochemistry (author's transl)].

15 ditetrazolium salts were examined to prove their qualities for histochemical techniques. The succinate dehydrogenase, the lactate dehydrogenase and the diaphorase I in hearts, muscles, livers, kidneys and brains of rats were demonstrated for it. The results show that NBT is the best allround tetrazolium salt for the histochemical demonstration of dehydrogenases. For the study of special questions it is suitable to use other tetrazolium salts, too.

Animals↗
PubMed1978

[On the histochemistry of xanthine dehydrogenase (author's transl)].

We have investigated the enzymes of the XDH-XOD-system by histochemical technique based on a model study for histochemical tetrazolium reduction by flavine enzymes. We have not found a reaction in native sections from liver, kidney and duodenum of adult male Wistar rats. The effectors PMS, Triton X-100 and Tween 80 were inefficient. A strong reaction was observed after a short fixation (10 min) of the sections with glutaraldehyde predominantly in the duodenal epithel cells. The intensity of the reaction was much stronger in sections of liver, kidney and duodenum by influence of the effector PMS and the detergents Triton X-100 and Tween 80. The application of the membrane technique does not show advantages in the histochemical demonstration of the XDH-XOD-system. The specificity of the reaction is discussed under the aspect of the complexity of the XDH-XOD-system shown by biochemical investigations.

Animals↗
PubMed1978

[A improved demonstration of ischemic myocard injuries with a combined method for evidence of the succine dehydrogenase and the acridin-fluorchrome-technique (MS-AO-technique) (author's transl)].

A method is described for combined enzyme histochemical and fluorescence microscopical investigation of ischemic injuries. This new method allows to distinguish between 1 reversible and 3 irreversible zones at the experimental ligature infarct of 48 h duration. Heterolysis is discussed as reason for the 3 outer zones and autolytic processes for the central area.

Animals↗