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E Seidler

Publications and source records attributed to E Seidler.

At least 37 records · Page 2Linked to original sources
PubMed1986

[Not Available].

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Austria↗
PubMed1986

[Relation between chemical structure and histochemical qualities of tetrazolium salts].

For ascertaining correlations between the chemical structure of tetrazolium salts and their histochemical qualities, 97 of these indicators (72 monotetrazolium salts and 25 ditetrazolium salts) were used to demonstrate histochemically the succinic dehydrogenase activity in organs of rat. The nitro-derivatives of tetrazolium salts are of special value for histochemical use.

Animals↗
PubMed1986

[Enzyme histochemical demonstration of sodium-dependent glutamate uptake in glutamatergic brain structures in the rat].

Using a special tetrazolium salt technique, a striking correlation was observed between Na+ concentration of the incubation medium and the activity of glutamate dehydrogenase (GDH) in glutamatergic neuropil areas of the hippocampal formation, cerebellum, and other brain regions. Na+ concentration of 130 to 150 mmol/l caused maximal formazan production. The histochemical enzyme reaction in neuronal perikarya as well as biochemically estimated GDH activity were found to be rather inhibited by such Na+ concentrations. The GDH catalyzed sodium dependent increase in formazan production is discussed to be a consequence of the sodium dependence of glutamate uptake in glutamatergic brain structures supplying the enzyme with substrate.

Animals↗
PubMed1985

Biomedicine and naturopathic healing in West Germany. A historical and ethnomedical view of a stormy relationship.

We trace the history of medical pluralism in Germany from the perspective of a clinically oriented medical anthropology. The continuation of naturopathic medicine in both formal and informal health care illumines fundamental epistemological issues. Cultural and social forces in Germany shaped the scientific and technical development of medicine, which continues in a form distinctly different from that of medicine in North America. The construction of clinical reality in German practice is distinctive and edifying for a cross-cultural understanding of medical systems of knowledge and praxis.

Germany, West↗
PubMed1985

[Differences in the effects of laser radiation on the myocardium and liver induced by a combination of histochemical succinate dehydrogenase demonstration and acridine orange fluorochromy (MS-AO-technic)].

In laser induced wounds of myocardium and liver of rats, 1 reversible zone and 3 irreversible zones of damage can be separated in dependence of time until 24 h by a combined enzyme-histochemical and fluorescence microscopical technique. Besides these zones, another carbonized zone is also detectable. A mainly reversible zone of contraction can be seen for a short time in the myocardium. The results indicate different types of manifestation of laser and ischemic induced tissue necroses.

Acridine Orange↗
PubMed1984

Johann Friedrich Meckel the Younger (1781-1833).

On the occasion of the 200th anniversary of the birthday of Johann Friedrich Meckel the Younger (1781-1833) it is appropriate to examine his contributions to the field of clinical genetics. Special emphasis is given to his laws of "diversity" and "reduction." These deal respectively with the evolutionary and developmental differences between organisms and the reasons for similarities in development of parts of an organism and the parts of different organisms. Meckel is an important pioneer of modern clinical teratology because he did not restrict his studies to normal development, but rather concentrated on its aberrations in his reflections on the history of development.

Anatomy↗
PubMed1984

[Value of histochemical tetrazolium technics].

A short review is given about the value of the tetrazolium technique in histochemistry including some new aspects of the tetrazolium reduction. The different factors and properties determining the localization, pattern and colours of the formazans in the tissue slide are discussed. The current knowledge of the atypically coloured formazans caused by the nitro groups is presented.

Formazans↗
PubMed1984

[Improved demonstration of topochemical dehydrogenase in the CNS with a nitro-monotetrazolium salt].

Succinate dehydrogenase (SDH) and glutamate dehydrogenase (GDH) were determined parallel to the tetrazolium salts p-DNTT, NBT and TNBT under different conditions using sagittal brain sections of newborn and 3 till 100-day-old rats. Under conventional incubation conditions for SDH (37 degrees C, 30 min), the tetrazolium salts p-DNTT stained more intensely than NBT or TNBT. Decrease of the final concentration of tetrazolium salts from 1.2 mmol/l to 0.6 mM effected a considerable increase of p-DNTT formazan development. Using NBT or TNBT, the formazan formation was significantly smaller. In contrast to SDH the demonstration of GDH activity under incubations conditions mentioned is unsatisfactory at any rate. Preservation of mitochondria during the preparation and incubation procedure improved evidently the quality of the GDH-demonstration, especially by use of p-DNTT. The formazan of p-DNTT was formed to be amorphous and was confined exclusively to enzyme containing compartments, whereas formazanes of the NBT and TNBT spread out more diffusely.

Animals↗
PubMed1983

[Histochemical detection of tissue changes using tetrazolium salts of differing nitrosensitivity].

By histochemical detection of succinic dehydrogenase in the myocardium of rats, it was shown that cell alterations induced by isoprenaline can be demonstrated in different qualities by the use of the atypical formazans of suitable nitro-monotetrazolium salts. The chemical structure of these nitro-monotetrazolium salts defines their different trends ("nitro-sensitivity") to react in their atypically-coloured formazans under histochemical conditions. A range of nitro-monotetrazolium salts with graded nitro-sensitivities is stated for improved histochemical detection of cell and tissue injuries.

Animals↗
PubMed1982

[The importance of the superoxide dismutases in histochemistry of dehydrogenases (author's transl)].

During the incubation of cryostat sections (various organs of rat) with aqueous media xanthine dehydrogenase and superoxide dismutase activities become soluble and detectable in the solution by biochemical methods. Using the tetrazolium technique the demonstration of xanthine dehydrogenase is successful only after separation from the superoxide dismutase by gel electrophoresis. The soluble flavine enzymee xanthine dehydrogenase is taken as an example to discuss the significance of superoxide dismutases in incubation media for the histochemical demonstration of dehydrogenating enzymes by tetrazolium methods.

Animals↗
PubMed1982

[Nonenzymatic models of histochemical tetrazolium reduction (author's transl)].

4 nonenzymatic biochemical systems (NADH-mediator-tetrazolium; NADH-riboflavin-tetrazolium; methionine-riboflavin-tetrazolium; potassium superoxide (KO2)-tetrazolium) were tested as models for histochemical tetrazolium reduction. A NADH-PMS-tetrazolium system was proved to be most suitable. The reaction rates of some histochemically used tetrazolium salts, the stimulation of the reaction rates by the detergents Tween, Triton X-100, and N-Cetylpyridinium chloride and the effectiveness of some histologic dyes (oxazines, phenazines, thiazines) as mediators were determined as examples of application. The reported results are discussed in relation to the role of detergents and oxygen in the mechanism of tetrazolium reduction.

Coloring Agents↗