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Biomedical subjects

E Redondo

Publications and source records attributed to E Redondo.

At least 55 records · Page 3Linked to original sources
PubMed1993

Location of bovine respiratory syncytial virus antigens in the lung of experimentally-infected lambs: comparative study using indirect fluorescent antibody test, avidin-biotin-peroxidase complex and transmission electron microscopy.

The experiments were designed to analyse the efficiency of the Indirect Fluorescent Antibody Test (IFAT), the Avidin-Biotin-Peroxidase Complex (ABPC) and Transmission Electron Microscopy (TEM) in detecting Bovine Respiratory Syncytial Virus (BRSV) antigen in ovine lung. 25 gnotobiotic Merino lambs each received an intratracheal injection of 20 ml of BRSV infected cell culture suspension (strain NMK-7) diluted at the concentration of 1.26 x 10(6) TCID50/ml. Results showed that the immunological techniques were more efficient than transmission electron microscopy in detecting BRSV antigen. IFAT and ABPC yielded identical results. However, difficulties in interpreting aspecific fluorescence, coupled with the ease of identification of pneumonic areas from paraffin-embedded tissues, suggest that ABPC is the most suitable technique for identifying BRSV antigen in the lungs of experimentally-infected sheep.

Animals↗
PubMed1993

Ontogeny and distribution of gastrin cells in the gastrointestinal tract of the sheep.

Indirect immunofluorescence and peroxidase-antiperoxidase methods were used to analyze the development and distribution of gastrin-producing cells in 24 embryos and fetuses (at 26-120 days of prenatal life) of sheep and in 10 newborn sheep. Plasma gastrin concentrations were measured by radioimmunoassay in blood samples taken for the immunocytochemical study. Gastrin cells first appeared at 69 and 77 days of gestation in the duodenum and pyloric antrum, respectively. Gastrin immunoreactive cells were very numerous in the pyloric region, numerous in the duodenum, scarce in the jejunum and rare in the ileum. None were detected in the cardiac or fundic regions of the abomasum or in the colon or rectum. Plasma gastrin concentrations were first detected at 69 days, increasing gastrin levels being observed during the development stages just before birth. The differences between sheep and other species in the distribution and development of these endocrine cells are discussed.

Animals↗
PubMed1993

[Thyroid lymphoma in a 51-year-old woman].

Thyroid gland is an uncommon site for involvement by lymphoma. Because of the lack of specific histopathologic criteria for diagnosis, thyroid lymphomas are not usually diagnosed until a thyroidectomy is done, even when a fine-needle aspiration biopsy is performed before surgery. We report the case of a woman with a non-Hodgkin lymphoma presenting as thyroid enlargement with systemic manifestations of the disease. The preoperative histologic diagnosis was poorly differentiated thyroid carcinoma. If the lymphoma was primarily thyroid or the involvement of thyroid was a part of the generalized dissemination of a nodal non-Hodgkin lymphoma is difficult to establish. She was treated with a combination of surgery and chemotherapy with excellent outcome. We comment the problems in the diagnosis of this disease.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1993

Histomorphometric analysis of the omasum of sheep during development.

Histomorphometric and scanning electron microscopic analyses were performed on 74 embryos and fetuses and 20 sheep (early postnatal to adult age). Histologic differentiation of the omasum took place at 33 days of fetal life, with the appearance of first-order laminae. Second-, third-, and fourth-order laminae appeared at 39, 50, and 59 days, respectively. Neutral mucopolysaccharides first appeared in epithelial cells at 46 days of fetal life, decreasing quantitatively until birth, before subsequently stabilizing in postnatal life. Acid mucopolysaccharides, mucins, and mucoid compounds were not detected. Growth curves and formulas were constructed for each tissue layer. Initial tests involved multiplicative (y = axb), exponential (y = EXP [a + bx]), linear (y = a + bx), and polynomial models (y = a + bx + cx2 + dx3).

Animals↗
PubMed1993

Histomorphometric analysis of the reticulum of the sheep during development.

Histomorphometric and scanning electron microscopic analysis were performed on 74 embryos and foetuses and on 20 sheep (early post-natal to adult age). Histodifferentiation of the reticulum took place at 33 days of foetal life. Reticular ribs were observed as evaginations of the epithelial stratum germinativum at 64 days. Neutral mucopolysaccharides first appeared in epithelial cells at 46 foetal days, thereafter to decrease gradually in number, subsequently stabilising in postnatal life. Acid mucopolysaccharides, mucins and mucoid compounds were not detected. Growth curves and formulae were constructed for each tissue layer. Initial test involved multiplicative (y = axb), linear (y = a+bx) and polynomial model (y = a+b+cx2+dx3).

Animals↗
PubMed1993

Experimental reproduction of acute pneumonic pasteurellosis in rabbits.

A histomorphometric and physiopathological study was made of the lung parenchyma of Belgian White SPF rabbits infected experimentally with Pasteurella multocida type A. Symptoms observed were characteristic of the acute respiratory syndrome. Mean serum cortisol concentration and rectal temperature increased in all experimental groups. Histopathological changes included alveolitis and leukocytic bronchitis. Changes in alveolar and bronchial cytoarchitecture were attributed to the degeneration and necrosis of constituent epithelial cells.

Acute Disease↗
PubMed1992

Linear unilateral basaloid follicular hamartoma.

A 36-year-old woman with a linear and unilateral cutaneous hamartoma is described. The lesions consisted of hypopigmented macules that coalesced into a linear plaque that involved only the left side of her body. Within this plaque were several pearly papules and an ulcerated area that clinically resembled basal cell carcinomas. Histologic features of the hypopigmented linear plaque included multiple and multifocal basaloid proliferations in the papillary and mid dermis. The isolated pearly papules showed the typical findings of basal cell carcinoma.

Adult↗
PubMed1992

Pathological changes in kidneys of dogs with natural Leishmania infection.

A study was made of the nephropathy in canine leishmaniasis produced in ten adult dogs naturally infected with Leishmania infantum. Renal function analyses were performed (uraemia, creatinaemia, plasma proteins, biochemistry and urinary sediment), the humoral immune response (fluorescent antibodies and levels of serum IgG, IgM and IgA) was assessed and histopathological studies were carried out. Correlation of the results showed acute renal insufficiency which was reversible in two animals (endotheliomesangial glomerulonephritis) and irreversible in four cases corresponding to glomerulonephritis in its Type I and Type II proliferative forms; extensive increase in the glomerular basal membrane, proliferation of mesangial cells and growth of the mesangial matrix were observed, as was a widespread incidence of immune complex deposits. Two animals showed chronic renal insufficiency. Lack of renal changes (minimal-changes glomerulonephritis) in two dogs was accounted for in one animal by an almost complete absence of symptoms and in the other by chronic viscerocutaneous symptoms; neither showed more than a slight immunoglobulin response.

Acute Kidney Injury↗
PubMed1992

Adenocarcinoma of the ethmoid olfactory mucosa: a histopathological and ultrastructural study with evidence of virus-like particles.

A histopathological, morphometric and ultrastructural study was made of enzootic nasal neoplasia in a group of 25 Verata goats. A nasal adenocarcinoma was diagnosed. The neoplasm contained two clearly defined zones, one cystic and the other compact. The tumour stroma was composed of abundant loose connective tissue, in which mononuclear infiltrate was clearly identifiable. The observed viral particles were morphologically similar to Visna-Maedi. These particles had an eccentrically located electrodense core. The diameter of the virus was about 90 nm and it showed envelope numerous spikes on the surface. The virus is assumed to be the causative primary agent of the tumour.

Adenocarcinoma↗
PubMed1992

Histomorphometric analysis of the rumen of sheep during development.

Histomorphometric and scanning electron microscopic analyses were carried out on 74 embryos and fetuses and 20 sheep (early postnatal to adult age). Histodifferentiation of the rumen took place at 33 days of fetal life. Ruminal pillars were observed at 42 days, and at 61 days, ruminal papillae appeared as evaginations of the epithelial stratum basale. Neutral mucopolysaccharides first appeared in epithelial cells at 46 days of fetal life; thereafter, numbers decreased gradually and subsequently stabilized in postnatal life. Acid mucopolysaccharides, mucins, and mucoid compounds were not detected. Age and diet were recognized as factors that determine the structure of the ruminal mucosa. Growth curves and formulas were set out for each tissue layer.

Animals↗
PubMed1991

Histopathological changes in sheep experimentally infected with Babesia ovis.

Histopathological study was made of 12 Merino sheep - five splenectomized and seven intact - experimentally infected with Babesia ovis. Non-purulent encephalitis; initially exudative and subsequently interstitial pneumonia; pericarditis, myocarditis and haemorrhagic endocarditis; centrilobular necrotic hepatitis; hyperplasia of the lymphoreticular system; necrosis and vascular changes in adrenal glands were observed. The kidney was the most severely affected organ, exhibiting acute tubular necrosis typical of kidney shock syndrome. The lesions observed were suggestive of hypovolemic shock culminating in haemorrhagic diathesis owing to consumptive coagulopathy. Additionally, the massive release of catabolites from lysis and necrosis apparently produced endotoxic shock.

Abomasum↗
PubMed1990

Structural and ultrastructural differentiation of cell types in the gills of the tench (Tinca tinca, L.).

This study used 22 tench of 25-30 cm in length and weighing roughly 200 g. After sacrifice, the gill arch was extracted and fixed in 5% glutaraldehyde for structural, ultrastructural and morphometrical study. Tench gills consist of primary lamellae which are perpendicular to numerous secondary lamellae in which the respiratory barrier is located. A study was made of the structures which make up that barrier and of the multi-layered epithelium situated in the interlamellar space, which is closely linked to maintaining of internal balance in the fish.

Animals↗
PubMed1990

Experimental lead nitrate poisoning: microscopic and ultrastructural study of the gills of tench (Tinca tinca, L.).

A microscopic, ultrastructural, and morphometric study was made of the gills of tench (Tinca tinca, L.) subjected to acute experimental lead nitrate poisoning. Twenty-one adult tench were subjected to poisoning and a further 22 were used as controls. Lesions were characterized by the appearance of edema and epithelial hyperplasia and necrosis, both in cells forming part of the filtration barrier and in those in the interlamellar space. These processes developed in the course of the experiment, leading to the death of tench after 12 to 15 days of exposure to 75 ppm lead nitrate, at which point the concentrations of lead in the gills had reached their maximum.

Animals↗
PubMed1990

[Needle biopsy of pancreatic masses].

We report the results of 66 ultrasound or CT guided needle aspirations in 59 patients with pancreatic masses, from 1983 to 1987. Cytologic and histologic samples were obtained in 45 cases. Histologic samples, formed by minute tissue fragments, were paraffin embedded for standard sections (minibiopsy). Sensitivity, specificity and overall accuracy of the series as a whole were 88, 100 and 89.8%, respectively. We discuss the clinical usefulness of the technique; isolated cytologic and histologic results are compared and the minibiopsy contribution to the cytologic diagnosis is discussed.

Adult↗
PubMed1990

Ovarian sex cord tumor with annular tubules and Peutz-Jeghers syndrome.

A case of ovarian sex cord tumor with annular tubules (SCTAT) is described in a patient with Peutz-Jeghers syndrome. The patient had undergone surgery at age 19 years for intestinal invagination caused by intestinal polyposis, and the subsequently had seven pregnancies. Of the four children surviving at the time of this study, all had intestinal polyposis. The ovarian tumor was discovered during a routine examination, although the patient had suffered moderate menstrual disorders and hypermenorrhea for several months previously. Eight years after surgery the patient remains free of symptoms. A review of the bibliography shows that sex cord tumors with annular tubules are very rare. Initially described by Scully in 1970, these usually benign tumors appear frequently in patients with Peutz-Jeghers syndrome. They are considered to originate from granulosa cells, and grow in patterns reminiscent of Sertoli cells.

Adult↗