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Biomedical subjects

E Palacios

Publications and source records attributed to E Palacios.

At least 73 records · Page 4Linked to original sources
PubMed1996

Comparative evaluation of microplate enzyme-linked immunosorbent assay versus plaque reduction assay for antiviral susceptibility testing of herpes simplex virus isolates.

We tested the antiviral susceptibilities of 30 clinical isolates of herpes simplex virus using the microplate in situ enzyme-linked immunosorbent assay (MISE) and the plaque reduction assay (PRA). There was concordance for 26 of 30 acyclovir results and all 30 foscarnet results. MISE and PRA results each predicted the response to acyclovir in 12 of 14 instances and the response to foscarnet in 8 instances. MISE is more rapid than PRA, has an objective endpoint, and correlates well with the clinical response to therapy.

Acyclovir↗
PubMed1995

Effect of lipectomy and long-term dexamethasone on visceral fat and metabolic variables in rats.

Intraperitoneal (IP) fat accumulation in humans is a risk factor for a number of diseases. We tried to increase this particular adipose mass in rats by long-term administration of low-dose dexamethasone (Dex) and/or elimination of other fat depots. Male adult Wistar rats were lipectomized (Lip) or sham-operated (Sh). Bilateral lipectomy of retroperitoneal and inguinal fat pads was performed under anesthesia with Na pentobarbital 40 mg/kg supplemented with ether. After 8 days, half the animals of each group received Dex in their drinking water (0.1 microgram/mL) while the other half received water (W), for a total of four groups: Sh-W, Lip-W, Sh-Dex, and Lip-Dex. Body weight (BW) and food and water intake were measured throughout the treatment period. A glucose tolerance test was performed 34 days after starting Dex treatment, and then rats were killed, fat depots were weighed, and plasma and liver were obtained for metabolic determinations. Dex rats ate the same amount of food as W controls, but gained significantly less weight (2.02 +/- 0.18 v 3.82 +/- 0.10 g/d, P < .01). Mean daily W intake was approximately 40 mL/d in all groups, which means that Dex rats ingested approximately 4 micrograms/d Dex. Average glycemic values during the 180-minute glucose tolerance test were as follows: Sh-W, 162 +/- 13; Lip-W, 166 +/- 7; Sh-Dex, 118 +/- 6; and Lip-Dex, 229 +/- 27 mg/dL. These values show that glucose tolerance was improved by Dex treatment alone, but was impaired in Lip-Dex animals. The same trend was evident for the relative weights (percent of BW) of two intact adipose depots: IP and epididymal (EPI) (Sh-W, 2.08 +/- 0.13 and 1.35 +/- 0.11, respectively; Lip-W, 1.67 +/- 0.15 and 1.17 +/- 0.11; Sh-Dex, 1.66 +/- 0.10 and 1.28 +/- 0.07; Lip-Dex, 2.41 +/- 0.11 and 1.53 +/- 0.09). Average glycemia for all rats was significantly correlated with IP (r = .55, P < .01) but not with EPI; moreover it was correlated in the Sh-W control group (r = .81, P < .05), suggesting a normal relation between these variables. Liver triglycerides (LTG), which were elevated in Dex rats, were also correlated with IP (r = .51, P < .02 for all rats and r = .82, P < .05 for Sh-W rats). The results show that long-term administration of low-dose Dex has some different effects in normal versus Lip rats concerning mainly the IP fat depot, the relative mass of which seems to significantly affect glucose tolerance.

Adipose Tissue↗
PubMed1995

Norepinephrine inhibition of water and food intake: comparison with vasopressin effects.

UNLABELLED: In a previous publication we showed that intraperitoneally (IP) injected norepinephrine (NE) induces hypodipsia (hD) in rats by an alpha 1-adrenergic effect which might be due to splanchnic vasoconstriction. In the present work we administered two vasoconstrictive hormones: NE 250 ug/kg and arginine vasopressin (VP) 550 mU/kg either by IP or intramuscular (IM) route to fasted rats in two different thirst-inducing conditions: (a) water-deprivation; or (b) induced hyperosmolarity. IP NE inhibited significantly food and water intake under both conditions. IM NE did not affect food intake and elicited significantly less hD and this only in (a). VP did not affect food intake but induced hD regardless of the route of administration in (a) but not in (b). NE administrated to anesthetized rats after food and water deprivation increased arterial pressure by both routes while VP effect was weaker and more variable. IN CONCLUSION: blood pressure elevation may be implicated in the hD effect but IP NE elicits a specific splanchnic action; splanchnic-induced hypophagia is not necessarily related to water intake inhibition.

Animals↗
PubMed1993

Effects of dose limits reduction on the Argentine nuclear power plants.

Occupational doses are evaluated in different stages of the fuel cycle and in the operation of nuclear power plants. Trends in individual dose distribution and collective doses are analyzed. The most contributive working conditions to collective dose are identified and the implications of dose limit reduction recommended by the ICRP in 1990 are assessed. It is concluded that no relevant difficulties should appear in accomplishing the new recommendations except for implementation at Atucha I, a nuclear power plant designed in the 1960s. Some options to reduce individual and collective doses in this plant are analyzed. The change of fuel channels by new ones free from cobalt is essential to get effective improvement of occupational exposures.

Argentina↗
PubMed1990

Synthesis and structure of cytosine dichloride cuprate: direct binding of copper to cytosine.

Dichlorobis(cytosine)copper(II), [CuCl2-(C4H5N3O)2], Mr = 356.65, monoclinic, P2(1)/n, a = 8.399 (2), b = 13.773 (2), c = 10.775 (2) A, beta = 90.8 (2) degrees, V = 1246.3 (9) A3, Z = 4, Dx = 1.901 Mg m-3, lambda (Mo K alpha) = 0.7107 A, mu = 2.195 mm-1, F(000) = 716, T = 298 K, R = 0.032, wR = 0.037 for 2313 reflections greater than 2 sigma(F). The crystal structure of the title compound consists of two crystallographically independent cytosine molecules attached together through the direct bonding of a Cu2+ ion with two N atoms of the pyrimidine rings. In addition to the N atoms, the Cu ions are coordinated to two Cl atoms forming approximately a square-planar environment. The bond distances of Cu to Cl(1), Cl(2), N(3) and N(3') atoms are 2.299 (1), 2.267 (1), 1.985 (4) and 1.996 (3) A, respectively. Packing of the Cyt(I)-CuCl2-Cyt(II) complex in the crystal is ensured by an extensive extramolecular N--H...Cl and N--H...O network. Hydrogen bonds involving Cl, O and H atoms of the neighboring Cyt(I) and Cyt(II) induce a slight distortion in Cu--Cl, C = O distances but do not significantly affect intramolecular bond lengths and angles in the cytosine rings and their flatness.

Chemical Phenomena↗
PubMed1990

Biliary cystadenoma: ultrasound, CT, and MRI.

Two cases of intrahepatic biliary cystadenoma, a rare tumor with malignant potential, are discussed. The sonographic, computed tomographic (CT), and magnetic resonance imaging (MRI) findings are presented together with clinical features of this entity and a brief review of the pertinent literature.

Bile Duct Neoplasms↗
PubMed1989

Effect of arthroscopic temporomandibular joint surgery on articular disk position.

This study used magnetic resonance imaging to assess disk-condyle relationships before and after arthroscopic surgery. Disk positions relative to condyles were found to be generally unchanged, despite the greater range of opening. Clinical findings were positive symptomatic changes with improved interincisal openings in 11 of 12 patients (92%). The results suggest that the clinical success of arthroscopic surgery as a treatment modality does not occur as a result of disk repositioning or recapturing.

Adult↗
PubMed1987

Hippocampal formation and related structures of the limbic lobe: anatomic-MR correlation. Part I. Surface features and coronal sections.

Correlation of magnetic resonance (MR) images with cryomicrotome sections and formalin-fixed sections of human brain documents that spin-echo MR imaging with short repetition time/short echo time pulse sequences can display the surface gyri of the inferomedial temporal lobe, including the temporal pole, the parahippocampal gyrus, the isthmus of the cingulate gyrus, the lateral and medial occipitotemporal gyri, and the inferior temporal gyrus; the associated sulci including the rhinal and collateral sulci, the occipitotemporal sulcus, and the anterior calcarine sulcus; the components and fiber tracts of the hippocampal formation including the pes hippocampi, the hippocampus, the subiculum, the dentate gyrus, the subsplenial gyri, the alveus, the fimbria, and the fornix; the fissures and sulci associated with the hippocampal formation, including the hippocampal fissure, dentatofimbrial fissure, and the choroidal fissure; the continuity between the cingulate gyrus and the parahippocampal gyrus through the isthmus of the cingulate gyrus; and the continuity between the callosal sulcus and the hippocampal fissure.

Hippocampus↗
PubMed1987

Hippocampal formation and related structures of the limbic lobe: anatomic-MR correlation. Part II. Sagittal sections.

Magnetic resonance (MR) images in the sagittal plane display the lengths of the parahippocampal gyrus, subiculum, dentate gyrus, hippocampus, fimbria, fornix, hippocampal fissure, choroidal fissure, and temporal horn, and the anatomic relationships of these structures to the surrounding brain. Correlation of these images with anatomic specimens provides criteria for identifying these structures confidently on routine clinical MR imaging.

Hippocampus↗
PubMed1987

Malignant mesothelioma with occupational and environmental asbestos exposure in an Illinois community hospital.

Clinical, radiologic, pathologic, and epidemiologic data on 32 patients with diffuse malignant mesothelioma (DMM) diagnosed between 1968 and 1984 at a 427-bed community hospital in Berwyn, Ill, were reviewed. Independent pathologists' review of light microscopy, supported by electron microscopy, immunoperoxidase staining, or autopsy, confirmed 29 pleural and three peritoneal DMMs. Clinical and radiologic characteristics were similar to those in published case series. Median age at diagnosis was 67 years, and median survival after diagnosis, seven months. Fourteen patients were women. Exposure histories were obtained through 22 interviews supplemented by hospital charts and death certificates. Thirty patients (94%) had a history of asbestos exposure through work (15 [47%]) and/or residence near an asbestos facility (27 [84%]). Medical records and death certificates underreported asbestos exposure and DMM.

Adult↗
PubMed1982

Malignant lymphoma of the nervous system.

The radiological findings in 22 patients with malignant lymphoma of the nervous system are reviewed in relation to the clinical and histological features. The radiological findings were grouped into four major categories: intracranial, orbital, nasopharyngeal, and spinal. A fifth category, complications, was considered separately. The common neuroradiological manifestations of nervous system lymphoma are reviewed and compared with the recent literature.

Adolescent↗
PubMed1982

Serial CT scans in Haemophilus influenzae meningitis of childhood.

Eleven children admitted to hospital with H. influenzae meningitis had computerized tomographic head scans during the acute stage of the illness. 10 of the 11 had at least one other scan between two weeks and 30 months later. This study evaluated the changes seen in the scans in relation to the evolution of the illness, especially with reference to subdural effusions and communicating hydrocephalus. The early scans were found to have no significance in predicting clinical outcome. However, a temporary developmental lag occurred in those children with subdural effusions, transient communicating hydrocephalus, and without permanent neurological deficit.

Brain↗
PubMed1982

[Changes in lipid metabolism which accompany thioacetamide-induced cirrhosis].

Intraperitoneally-administered thioacetamide to rats produces the incorporation of 3H2O into the liver lipids during eight days, diminishing thereafter together with a decrease in 32P incorporation to phosphatidyl choline. Contrarily, the sphingomyelin incorporated 32P increases by the effect of the toxic action of the drug, while the 3H binding decreases. The cirrhosis-inducing agent possibly produces a shunt of the common CDP-choline precursor to the biosynthesis of sphingomyelin with detriment to the synthesis of phosphatidyl choline. The role of the unsaturation of fatty acids by thioacetamide is considered.

Acetamides↗
PubMed1982

[Effect of thioacetamide on the incorporation of tritiated water into rat liver phospholipids].

Male Wistar rats received an intraperitoneally daily dose of thioacetamide of 100 mg/kg body weight during 3, 8 and 30 days. Phospholipid levels and radioactive incorporation of 3H2O into total lipids, polar lipids and phospholipids 1 h after intraperitoneal administration of 5 mCi of tritiated water, were determined in livers of untreated control rats and of animal treated during the above mentioned periods. Earlier doses of thioacetamide provoked an increase in the 3H2O incorporation into total lipid fraction. Prolonged treatment resulted in an inhibition of tritiated water incorporation. After three days of thioacetamide administration the incorporated radioactivity in phospholipid fraction decreased. There was also observed a decrease in the radioactivity incorporated into the major phospholipids: phosphatidylcholine and phosphatidyl ethanolamine. However, the levels of these phospholipids showed no significant changes. These observations support the suggestion that de novo biosynthesized fatty acids are being utilized for 1-palmitoyl-2-oleoyl and 1-palmitoyl-2-linoleoyl phospholipids biosinthesis in lesser proportion in rats treated with thioacetamide than in control rats.

Acetamides↗