[Behavior of magnesium in pregnant women. I. Comparative study between normal pregnant women and those with pathology].
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Blood Proteins↗
Biomedical subjects
E Moreno
Publications and source records attributed to E Moreno.
Left ventricular function during haemofiltration and haemodialysis: a comparative study.
The relationship between systemic haemodynamics and left ventricular performance during HF and HD was evaluated by M-mode echocardiography. In patients with normal cardiac function and similar rates of fluid removal, special attention was paid to the effects of solute transport and buffer. Convective transport, independent of the replacement buffer, produces an increase in systemic vascular resistance with no changes in ventricular function. In contrast, a diffusive transport induces different effects depending on the dialysate buffer: acetate decreases vascular resistance and bicarbonate significantly improves myocardial contractility shifting the cardiac function curve to the left.
Blood↗
[Malignant epidermoid cyst of the maxilla].
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Aged↗
Gastroplasty with partial or total plication for gastroesophageal reflux: manometric and pH-metric postoperative studies.
From January, 1975, to December, 1980, 83 patients with sliding hiatal hernia, gastroesophageal reflux, or both were treated using a modified Collis gastroplasty associated with either partial or total gastric application. When partial plication was used, the five-year clinical results were considered satisfactory in 27 out of 35 patients (77%). When total plication was used, the results were satisfactory in 41 out of 46 patients (89%) after follow-up ranging from 12 to 60 months (average, 36 months), but no symptoms of gastroesophageal reflux reappeared in any patient. In patients undergoing partial plication, the mean preoperative high-pressure zone of 11.20 +/- 8.19 mm Hg increased after operation to 17.31 +/- 10.50 mm Hg, but in the second postoperative studies the value decreased to 13.69 +/- 7.24 mm Hg. When 360 degrees plication was used, the preoperative value of the high-pressure zone--9.36 +/- 4.80 mm Hg--increased after operation to 17.70 +/- 7.53 mm Hg but did not decrease significantly in the second postoperative studies: 16.46 +/- 7.99 mm Hg. When partial plication was used, the positivity of the abdominal compression test was 9 and 28% in the early and late postoperative studies, respectively. Using total plication, the percentage of positivity in the early and late postoperative periods was 0 and 2%, respectively. Concerning the acid reflux test, when partial plication was used, the test was positive in 3 out of 27 patients (11%) in the early postoperative studies and in 7 out of 30 (23%) one year later. For the total plication procedure, the percentage of positive tests was null in the first control and 3% in the second postoperative studies.
Deglutition Disorders↗
In vitro evaluation of editempa on hydroxyapatite formation and its effects on dental enamel.
N,N,N',N' ethylene diamine tetra (methylene phosphonic acid)-Editempa inhibited the formation of hydroxyapatite (HA) in vitro at 4 ppm. In the seeded crystal growth of HA at 37 degrees C, it completely inhibited the crystal growth at 0.5 ppm. C14-Editempa adsorbed to HA crystal at 37 degrees C. The maximum adsorption was 1.29 mumol/m2 of HA and the adsorption was monolayer. Compared to the natural inhibitors of HA in saliva (Statherin and Proline-rich proteins), Editempa was more effective in vitro on weight basis. At 0.3 ppm, it inhibited growth rate of the crystals by 80% while 4 to 5 ppm of the natural inhibitors were needed to get the same effect. The inhibition of rates with Editempa was not directly proportional to the area covered by it on the HA seeds (occupied less than 10% of the surface). The natural inhibitors, on the other hand, seemed to cover all the available surface. These data indicate that the inhibition with Editempa was due to binding to specific sites on the surface of the seeds. The solutions up to 5% of Editempa did not damage or etch the surface of human dental enamel in vitro at pHs 5.0 and 7.5, as evaluated by scanning electron microscopy and calcium released in the exposed solutions.
Adsorption↗
Spontaneous rupture of an intra-aortic balloon pump.
Circulatory assistance with an intraaortic balloon pump is not free from risks and complications. Among the latter, rupture is extremely rare. We report a case of spontaneous rupture of an intraaortic balloon, which occurred after 29 h of counterpulsation in a patient with cardiogenic shock following an acute myocardial infarction. The probable cause of the rupture was the constant abrasion and eventual perforation of the balloon membrane by a sharp calcified aortic plaque.
Aged↗
Immunochemical characterization of Brucella lipopolysaccharides and polysaccharides.
Purified lipopolysaccharide (LPS) extracted with phenol-water from smooth Brucella abortus was hydrolyzed with 1% acetic acid at 100 degrees C. The degraded polysaccharide (AH) released gave reactions of identity with the native polysaccharide hapten (NH) in phenol-water- or trichloroacetic acid-extracted endotoxin preparations of B. abortus and with the polysaccharide (poly B) extracted by trichloroacetic acid from rough B. melitensis strain B115. Poly B was present in the soluble cytoplasmic fraction but not in the membrane fraction, of disrupted B115 cells. It could not be extracted from three rough mutants of B. abortus or from B canis or B. ovis cells. Both AH and NH shared determinants present on smooth LPS and missing from poly B. Sugars found in purified LPS, NH, and AH included mannose, glucose, quinovosamine, glucosamine, and 2-keto-3-deoxyoctonate. Poly B contained only a trace amount of quinovosamine and no 2-keto-3-deoxyoctonate detectable by the thiobarbiturate assay. Sera from some rabbits immunized with pure smooth LPS and some, but not all, cows infected with field strains of B. abortus recognized the determinants missing from poly B. A subclass-specific enzyme-linked immunoassay showed that most of the antibody in sera from infected cows which binds to smooth LPS and to NH is of the immunoglobulin G1 subclass.
Brucella↗
Biological activities of Brucella abortus lipopolysaccharides.
Purified lipopolysaccharide (LPS) from smooth (s) and rough (R) strains of Brucella abortus and lipid A isolated from S-LPS by mild acid hydrolysis were examined in several assays of biological activity. Brucella S- and R-LPSs and Brucella lipid A activated the complement cascade. Previously reported mitogenic activation by Brucella LPSs of spleen cells from endotoxin-resistant C3H/HeJ mice was confirmed and also produced by isolated Brucella lipid A. Mitogenicity was not inhibited by polymyxin B, and amino acid analysis showed no binding of polymyxin B to Brucella LPS under conditions in which mitogenicity of phenol-water-extracted Escherichia coli LPS was inhibited. S and R Brucella LPSs and lipid A all produced equivalent polyclonal stimulation of C3H/HeJ and C3H/HeAU spleen cells. Crude and purified LPS from S but not from R B. abortus was toxic for outbred mice, with 50% lethal doses approximately six times greater than that for E. coli LPS. S- and R-LPSs were abortifacient in pregnant outbred mice. S Brucella LPS was lethal for carrageenen-pretreated C3H/HeJ and C3H/HeAU mice, whereas only C3H/HeAU mice were killed by E. coli LPS. The data are consistent with the hypothesis that the unique fatty acid composition of Brucella lipid A is responsible for its biological activity in endotoxin-resistant C3H/HeJ mice. The participation of the protein strongly bound to the lipid A cannot be excluded, but its mode of action, if any, is different from that of the lipid A-associated protein of enterobacterial LPS.
Animals↗
The immune spectrum in patients with pulmonary tuberculosis.
The immune response to mycobacterial antigens of 65 patients with pulmonary tuberculosis was studied using delayed skin test reactions and enzyme-linked immunosorbent antibody assays. Evidence for a spectrum of immune response was found. Six of 35 patients studied during the first month of therapy had tuberculin skin test anergy. Anergy was not related to state of nutrition or extent of disease, but it may have been associated with radiographically acute disease. The 6 anergic patients had somewhat higher antibody titers to mycobacterial protein, but not polysaccharide antigens, than did nonanergic patients.
Acute Disease↗
Characterization of Brucella abortus soluble antigen employed in immunoassay.
A soluble antigen extract of Brucella abortus (BASA) has been prepared by the National Veterinary Services Laboratories and furnished to a number of workers who are examining antibody-mediated and cell-mediated immune responses of cattle infected with B. abortus. Three lots of BASA were examined. There were quantitative but not qualitative differences among lots by content of protein, total carbohydrate, hexose, fatty acid, and 2-keto-3-deoxyoctonic acid. The presence of smooth lipopolysaccharide was demonstrated by the presence of 2-keto-3-deoxyoctonic acid and lipid, by Limulus lysate gelation activity, and by formation of characteristic lipopolysaccharide precipitates in immunoelectrophoresis. A polysaccharide antigen as well as two nonsurface antigens, A2 and C, were also identified. BASA is a satisfactory antigen for use in the enzyme-linked immunosorbent assay since the smooth lipopolysaccharide component bound to polystyrene and functioned in the test. Normal murine spleen cells showed a mitogenic response to BASA similar to that produced by purified smooth lipopolysaccharide. BASA has been used in other laboratories to stimulate peripheral blood leukocytes from cattle infected with B. abortus. Because BASA is a mixture of antigenic components shown to have mitogenic effects in the mouse system, questions on the nature of its stimulatory effect on bovine cells are raised.
Animals↗
Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis.
A radial immunodiffusion (RID) test employing a polysaccharide antigen (poly B) was compared with tests currently used in the diagnosis of bovine brucellosis. Over 1,000 sera from vaccinated and infected cattle, all of which had been examined bacteriologically, were used to determine the sensitivity and specificity of the RID, card, Rivanol, and complement fixation tests. The RID test identified 90% of the cattle that were shedding Brucella in their milk. Although the complement fixation test was more sensitive, it was less specific than the RID test in cattle vaccinated as adults with Brucella abortus strain 19. A sensitive screening test, such as the card test, in combination with the RID test could be used in diagnostic laboratories, or even in the field, with little additional expense or technical expertise. An additional advantage is that the RID could be applied to sera from adult cattle as early as 2 months after vaccination, when postvaccinal agglutinins and complement-fixing antibodies may still be present. The indirect hemolytic test was used with some of the sera and was found to be a very sensitive test which could be useful in areas of low incidence but would not be practical for large-scale testing in adult-vaccinated herds.
Animals↗
Use of the ELISA to screen for anti-thymocyte and anti-beta 2-microglobulin antibodies in leprosy and SLE.
A report is given of the use of the enzyme-linked immunosorbent assay to measure antibody to preparations of human thymocyte membranes (HTMA) and to beta 2-microglobulin. The assay described is simple and rapid, and requires only small quantities of an easily stored membrane preparation. The advantages of this technique over conventional methods involving cytotoxicity are discussed. Raised levels of IgM antibody to beta 2-microglobulin were detected in sera from SLE patients. Raised levels of IgG and IgM antibody to HTMA were found in sera from most active lepromatous cases. Two of eight sera from SLE patients showed raised IgG anti-HMTA, but not raised IgM. An attempt was made to study the subclass of the IgG antibodies found, but when checked against purified human IgG myeloma proteins, the available anti-subclass sera were found to lack the necessary degree of specificity in this assay.
Antigens, Surface↗
[Does the liver changes the antigenicity of an organ?].
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HLA Antigens↗
[Motivation for studying health careers (author's transl)].
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Career Choice↗
A new species of Leishmania parasite from the Venezuelan Andes region.
A new form of Leishmania is described from the Venezuelan Andes region. L. garnhami n.sp. is proposed for this parasite with amastigote stages showing a peculiar and unique organelle seen with light and electron microscope. It produces cutaneous lesions in people living at a height of between 800 and 1,8000 m. in urban and rural areas; the disease is associated with Lutzomyia townsendi, the main anthropophilic sandfly in the region. The parasite is easily inoculable into hamsters and grows slowly in vitro in blood-agar media with glucose, but more prolifically without glucose.
Animals↗
Purification and characterization of smooth and rough lipopolysaccharides from Brucella abortus.
In an attempt to obtain pure and well characterized smooth lipopolysaccharide (S-LPS) and rough lipopolysaccharide (R-LPS), smooth and rough strains of Brucella abortus were extracted by two different modifications of the phenol-water method. S-LPS was obtained in the phenol phase, and R-LPS was obtained in the aqueous phase. Further purification was accomplished by treatment with enzymes, detergents, NaI as a chaotropic agent to separate non-covalently bound contaminants, and by gel filtration. The degree of purity of the molecules was determined by chemical and immunological analysis and by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels. Lipid identification by gas-liquid chromatography showed seven major fatty acids. Palmitic acid accounts for about 50%, stearic acid accounts for about 10%, and hydroxylated fatty acids account for less than 5% of total fatty acids. 2-Keto-3-deoxyoctonate but not heptose was detected in the sugar analysis. Protein was found to be firmly bound to S-LPS but not to R-LPS.
Antigens, Bacterial↗
Brucella abortus lipopolysaccharide is mitogenic for spleen cells of endotoxin-resistant C3H/HeJ mice.
Preparations of lipopolysaccharide (LPS) from rough and smooth strains of Brucella abortus were mitogenic for spleen cells of athymic nude mice, C3H/HeAU mice, and the endotoxin-resistant C3H/Hej mice. The mitogenic response induced by crude smooth-LPS (f5) was greater than that produced by purified smooth-LPS (f5p); however, the dose-response curves were similar for both preparations. The mitogenic activity of mouse spleen cells to both f5 and f5p was higher than that produced by stimulation with purified rough-LPS. The dose-response curves with rough-LPS were also qualitatively different from those produced with the preparations of smooth-LPS.
Animals↗
Depletion of monocytes from human peripheral blood leucocytes by passage through sephadex G-10 columns.
Passage of human peripheral blood leucocytes through Sephadex G-10 columns results in a complete depletion of monocytes. Microscopic examination and ingestion of latex particles failed to reveal any monocytes in the column-passed cells. The study of surface markers shows a slight enrichment of T-cells and a decrease of B-cells that is more pronounced in the IgG- and IgA-bearing lymphocytes. No changes were seen in the Fc receptor-positive cells. Con A-induced activation was significantly affected. Pokeweed mitogen activation was reduced 50% at optimal doses of mitogen whereas the response was unaffected at suboptimal doses. Mixed leucocyte reactions (MLR) were greatly depressed when monocytes were removed from either the responder or the stimulator cells, indicating an important role of monocytes both in the elicitation of the response and as auxiliary cells in the mixed leucocyte culture.
Blood Cell Count↗