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Biomedical subjects

E Mallet

Publications and source records attributed to E Mallet.

At least 145 records · Page 8Linked to original sources
PubMed1977

[Correlation of parathormone determination and bone biopsy in primary hyperparathyroidism].

Eleven patients with primary hyperparathyroidism confirmed by histologic examination were subjected to a comparative study of the results of quantitative determination of parathormone and of quantitative bone biopsy. Quantitative radioimmunologic determination of parathormone was carried out with the aid of an N-terminal specific anti serum, while the histologic features studied were the periosteocytic and osteoclastic absorption surfaces. On the basis of a combination of the results of these two techniques, the diagnosis could be made in all the cases studied. A statistically significant correlation was observed between the results of quantitative determination of parathormone and those of histologic examination of the surfaces of periosteocytic lacunae (r = 0.63; p = 0.05).

Bone and Bones↗
PubMed1976

[A new technic to evaluate the sensitivity of the renal parathyroid hormone receptor in man].

We propose a simplified assessment of response to parathyroid hormone which could replace the traditional Ellsworth Howard test for the differential diagnosis of pseudohypoparathyroidism in man. We have demonstrated that bovine parathyroid hormone in normal and hypoparathyroid subject increases plasma cAMP except in pseudohypoparathyroid subjects. It is an efficient, reliable method without any collection of urine samples.

Child, Preschool↗
PubMed1975

[Acute pericarditis. Study of 50 consecutive cases].

50 consecutive cases of pericarditis were studied. Idiopathic and viral pericarditis made up 36 p. cent of all cases. The occurrence of tuberculous or rheumatic conditions was even lower than that reported in similar series in the literature. Amongst the clinical signs of these diseases, emphasis should be placed upon the frequency of chest pain which was increased by deep inspiration in only three out of four cases. Circulatory problems associated with the pericarditis were, on the whole, minimal. However, a fall in blood pressure was seen in 6 cases and true collapse in 3 more. The electrocardiogram showed, in addition to the classical signs, sagging of the PR interval in 36 p. cent and transient atrial fibrillation in 4 cases. As far as biological tests are concerned, it should be noted that elevation of creatine phospho-kinase to levels 4 times greater than normal may be seen, creating problems with the differential diagnosis from myocardial infarction. The course was in general favourable though two deaths occurred in this series (4 p. cent), one with tuberculous pericarditis and the other with idiopathic pericarditis.

Acute Disease↗
PubMed1975

[Radio-immunoassay method for determination of parathyroid hormone. Use of a commercially available antiserum and an international standard (author's transl)].

A radio-immunoassay method of parathyroid hormone in peripheral circulation is described utilizing both commercially available antibody and bovine PTH preparation for iodination. As a standard, the bovine PTH delivered from the Medical Research Council (MRC) is used. Antibody-bound hormone is separated from free hormone by solid phase immunoprecipitation. Good cross reactivity of this antiserum to human PTH is shown, but because of the lack of human standard, it is demonstrated, that results may be expressed in reference to the MRC bovine hormone. The coefficient of interassay variation is 12% and the detection limit is 300 pg/ml. In every healthy subject, immunoreactive PTH is detected: the normal distribution range (n equals 31) was 400 to 1800 pg/ml. Two hypoparathyroid patients have undetectable levels. There is a slight overlap between normal and those from hyperparathyroid patients. The present method gives results expressed in international units (MRC standard). The reliability of the PTH radio-immunoassay is dependent on the characteristics of the antiserum. This method provides a basis for the establishment of PTH values with the possibility of comparing results in health and disease.

Animals↗
PubMed1975

[Characteristics of guinea pig anti-bovine parathormone antiserum used in radioimmunologic determination of human parathormone].

The cross-reaction for hPTH radioimmunoassay and the multiple forms of hPTH circulating, pose several problems in interpreting results. This communication reports a sensitive radioimmunoassay for hPTH utilising a guineapig antiserum which is reactive with the amino-terminal region retaining biological activity, and which cross-reacts completely with hPTH, indicating levels of hPTH expressed as bovine PTH equivalent that would be correct.

Animals↗
PubMed1974

Effect of bovine parathyroid hormone 1-34 fragment on renal production and excretion of adenosine 3', 5' monophosphate in man.

Biologically active bovine parathyroid hormone (b PTH) 1-34 fragment infused over one hour in normal subjects produced an immediate and sharp increase in the excreted fractions of filtered bicarbonate, sodium and potassium, followed by the return to pre-infusion levels as soon as the administration of b PTH was stopped. There was a gradual but steady increase in the excreted fraction of filtered phosphate but a decrease in the excreted fraction of filtered calcium and magnesium. The excreted fractions of these ions were still abnormal 150 minutes after the completion of PTH infusion. The urinary excretion of 3'5'-cyclic AMP increased immediately about one hundred-fold but returned rapidly to pre-infusion levels. Urinary clearance of cyclic AMP approximated glomerular filtration rate in control periods and was twenty to thirty times greater during b PTH infusion. In subjects overloaded with bicarbonate, b PTH brought about a decrease in bicarbonate T(m) and the same effects on the urinary excretion of other electrolytes. 3'5'-cyclic AMP excretion was clearly higher in control periods and reached higher levels during b PTH infusion when compared to subjects without an alkaline load. 3'5'-cyclic AMP excretion and fractional clearance were also clearly higher in subjects not given b PTH when control periods were compared to periods with bicarbonate infusion or after acetazolamide administration. During distal blockade obtained by simultaneous administration of chlorothiazide and ethacrynic acid, there was a delay in the rise of 3'5'-cyclic AMP excretion after b PTH administration. It can be concluded from these studies that the pattern of excretion of 3'5'-cyclic AMP is similar to that of bicarbonate, sodium and potassium. The increase of 3'5'-cyclic AMP excretion when urinary pH is above 7 suggests a diffusion trapping mechanism for the secretion into the lumen of this nucleotide. Distal diuretics used in distal blockade did not inhibit 3'5'-cyclic AMP production but delayed its secretion into urine.

Adenosine Monophosphate↗