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Biomedical subjects

E Lundgren

Publications and source records attributed to E Lundgren.

At least 199 records · Page 11Linked to original sources
PubMed1978

Isozymes of amino acid naphthylamidase in chronic meyloid leukaemia and erythroleukaemia.

Isozymes of amino acid naphthylamidase were investigated in 11 patients with chronic myeloid leukaemia (CML) and 1 patient with erythroleukaemia. Two isozymes were found in all cases (isozymes A and C) which also occur in normal granulocytes. An isozyme with intermediate mobility appeared in all the patients in two main forms (isozymes BF and BS), which both differed from the isozyme B found in normal granulocytes. This may therefore support the diagnosis. In 3 patients a shift occurred between isozymes BF and BS, the possible significance of which is discussed.

Aminopeptidases↗
PubMed1978

Modulation of immune responses in newborn and adult mice by interferon.

Interferon was found to have both suppressive and enhancing effects on the antibody response in newborn and adult mice. Evidence was obtained that these effects are primarily evoked during the initial steps controlling cell proliferation. Stimulation of thymus and spleen cells with a T-cell mitogen was enhanced by low doses and suppressed by high doses of interferon. Treatment of parental spleen cells with interferon before injecting them into immunized F1 hybrid mice resulted in an enhanced allogeneic effect. These results are compatible with the hypothesis that interferon affects T cells and has an immunoregulatory role, either by inhibiting the action of suppressor cells or by promoting immunological maturation.

Animals↗
PubMed1978

Interferon production in glia and glioma cell lines.

Interferon (IF) was produced in glia and glioma cell lines in titers comparable to those produced by human fibroblasts. It was inducible by both Sendai virus and polyriboinosinic:polyribocytidylic acid. "Superinduction" resulted in up to 500-fold-higher titers of IF. The IF appeared to be of the fibroblast type, as revealed by experiments using heat treatment, assay of antiviral activity in heterologous cell lines, and neutralization with specific antisera. Since large amounts of IF may easily be produced with glioma cell lines, such cells may be suitable for mass production of IF.

Cell Line↗
PubMed1976

Serum naphythylamidase isoenzymes during hormonal treatment. Electrophoretic and quantitative studies.

Alterations in serum naphthylamidase isoenzymes were studied by electrophoretic and quantitative methods in women treated with oral contraceptives and women treated with naturally occurring conjugated estrogens for climacteric symptoms. In women treated with oral contraceptives the appearance of extra isoenzyme components was accompanied by a distinct and significant increase in the total serum naphthylamidase activity, whereas in treatment with conjugated estrogens no such increase was found. The result suggests that combined oral contraceptives and natural estrogens affect the serum naphthylamidase pattern in different ways.

Adult↗
PubMed1976

Cell surface changes in HeLa cells as an indication of cell cycle events.

A HeLa cell line synchronized by double thymidine block and mitotic shake off was shown to have a characteristic surface morphology for each of the different cell cycle stages. Inhibitors of cell multiplication were used to arrest cells in specific cell cycle phases, and these cells had a surface morphology similar to that of synchronized cells in the same phase. The results indicated a close association between the cell surface topography and the cycles of DNA synthesis in the cell nucleus of this HeLa line.

Bleomycin↗
PubMed1975

Enzymatic heterogeneity of granules in human leucocytes.

Homogenates of highly purified polymorphonuclear leucocytes and of a mixture of mononuclear leucocytes and platelets from human blood were separated by differential and isopyknic centrifugation. A heterogeneity in granules containing digesting enzymes was found in both cell preparations. Enzymes typical of lysosomes were found in the two cell preparations in a similar density gradient. Granules of low density were indicated in polymorphonuclear leucocytes by alkaline phosphatase. In both cell preparations a third granule, of lower density, seemed to exist enriched in amino acid naphthylamidase, acid hydrolases and in polymorphonuclear leucocytes also alkaline phosphatase and lysozyme. A remarkable difference between the two cell preparations was the occurrence of amino acid napthylamidase in denser granules of polymorphonuclear only, although the nature of these granules could not be determined.

Alkaline Phosphatase↗
PubMed1975

Subcellular distribution of amino acid naphthylamidase isozymes in human leucocytes.

Three isozymes (A, B and C) of amino acid naphthylamidase were found in human leucocytes. Isozyme B and C had close biochemical similarities and differed from isozyme A. Isozyme A was localized in the cytosol. Results obtained by combining electrophoresis with gradient centrifugation suggested that isozyme B originated in granules of polymorphonuclear leucocytes enriched in alkaline phosphatase. Isozyme C was found in granules of low density of polymorphonuclear as well as mononuclear leucocytes.

Alkaline Phosphatase↗
PubMed1975

Effects of variations in calcium concentration on parathyroid morphology in vitro.

Morphologic examinations, including the use of the potassium pyroantimonate-technique and x-ray analysis for ultrastructural localization of cations, were carried out on parathyroid glands from Mongolian gerbils cultured for 2 to 6 days. At a high calcium concentration most (suppressed) chief cells were characterized by a moderate or high cytoplasmic density, inconspicuous endoplasmic reticulum and Golgi complex, numerous often enlarged mitochondria, and the occasional occurrence of accumulations of secretory granules, storage granules, and lysosomal bodies, whereas other (atrophic) cells were smaller and still more dense. At a low concentration of calcium some (stimulated) chief cells were characterized by a low cytoplasmic density, normal or structurally altered mitochondria, and smooth surface vacuoles occasionally connected with mitochondria, whereas the main features of other (active) chief cells were a moderate or low cytoplasmic density and a prominent endoplasmic reticulum and Golgi complex. Calcium-containing precipitates were found both inter- and intracellularly; the latter were mainly localized to nuclei, mitochondria, and rough surfaced vacuoles, and in stimulated cells also to the smooth surfaced vacuoles and the cytosol. Degenerative changes were seen, mainly in the suppressed cells; massive calcium loading in mitochondria seemed to precede degeneration and necrosis of whole cells. The findings indicate that clear differences exist between the different chief cell variants both as to the distribution of calcium-containing precipitates and to the structural appearance, and that these differences are influenced by the ambient calcium concentration. Suppressed cells occasionally seem to accumulate hormone which later may undergo lysosomal digestion. Calcium might be accumulated intracellularly, mainly in mitochondria of suppressed cells.

Calcium↗