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Biomedical subjects

E Kuhn

Publications and source records attributed to E Kuhn.

At least 55 records · Page 3Linked to original sources
PubMed1982

Changes in the circadian rhythm of serum iron induced by a 5-day sleep deprivation.

The effect of 5 days of sleep deprivation on the circadian rhythm of serum iron was studied in a group of six healthy male volunteers. The results were compared with a control group of five individuals, whose normal sleep cycle was preserved, but whose daily regimen was otherwise identical with the sleep deprivation group. Their biorhythm was processed mathematically using cosinor analysis. Sleep deprivation markedly reduced the mean level of iron, diminished the absolute and relative amplitude of oscillations, disturbed the shape of the daily course of serum iron and gradually decreased the computative acrophase, i.e., shortened the period of rhythm. Forty-eight hours of recovery resulted only in a partial normalization of all the observed changes. The potential mechanisms of the observed changes are discussed.

Adult↗
PubMed1982

Johns Hopkins University, Baltimore, Maryland.

The STEM can be used in one of three modes: 1) to image individual atoms; 2) to measure mass or molecular weight; 3) to collect electron energy loss spectra or x-ray fluorescence data. Heavy atom imaging is used to identify chemical groups in a molecule or macromolecules in an assembly. Specific labels have been developed for bases in nucleic acids. These permit localization of bound proteins on single strand nucleic acids. Pt(gly-L-met)Cl is a specific label for methionine residues of proteins as shown with the SLS aggregate of collagen. Lysine can be labeled as well if first methyl (methyl-thio-acetimidate) is coupled. This labeling procedure permits the localization of individual histones within a nucleosome. Mass determination can be used to answer crucial questions about biological assemblies. This is demonstrated by examples from muscle structure.

Amino Acids↗
PubMed1982

The effect of stress on circadian rhythms.

The authors chose four types of intensive stress in man and show their effect on the circadian rhythms of selected parameters. Sleep deprivation reduces mean sideraemia and oscilation amplitudes. The morning rhythm maximum shifts to early morning hours. Acute fasting does not change the biorhythm of serum iron despite that mean sideraemia increases. On the other hand, realimentation is associated with a marked drop of iron level and a shift of the morning maximum to early afternoon hours. Stress induced by isolation in humid warm environment initiates a decrease of systolic blood pressure. While biorhythm amplitude remains unchanged peak systolic pressure moves from the usual 18 to 20 hours up to 23 to 24 hours. Stress caused by diagnostic cardiac catheterization results in biorhythm inversion of the urinary excretion of catecholamines and 17-OH-corticoids. On the day of catheterization, performed in all cases in the morning hours, the usual morning peak values of adrenaline shifted to afternoon hours and those of noradrenaline and 17-OH-corticoids even to late night hours. For practical purposes, biorhythm changes can be used as indicators of the effect and intensity of stress.

17-Hydroxycorticosteroids↗
PubMed1981

[Glycogenosis caused by amylo-1,6-glucosidase deficiency. Myopathy as a lead finding in adults].

Glycogen storage disease due to amylo-1,6-glucosidase deficiency was diagnosed in a 21-year-old patient. The enzyme defect was demonstrated by biochemical analysis of muscle tissue, the glycogen content of which was typically increased. Investigation of the patient's kindred showed that his 25-year-old sister was also affected. This report sets out to show that in adolescence and in adult life myopathy may be the leading symptom of the disease. Besides the clinical symptoms of muscle weakness and stiffness, an increase in serum creatine kinase usually is found. While an increase in the glycogen content of skeletal muscle has been known since the first description of this glycogen storage disease, it was believed that the glycogen deposits do not cause a clinically relevant disturbance of muscle function. A review of the literature and our own observations show that this assumption has to be at least partially revised. In patients with unclear myopathy who had hepatomegaly during childhood the possibility of glycogenosis due to amylo-1,6-glucosidase deficiency should be considered, especially if symptoms of hypoglycemia are reported. In the patient as well as in his sister marked kyphoscoliosis was present. Whether there is a connection between skeletal deformity and enzyme defect cannot be determined as the patients were available for further studies.

Adult↗
PubMed1981

A new type of distal myopathy in two brothers.

Two brothers are described with a distal myopathy different from the known hereditary distal myopathies. Early adult onset, beginning in the distal leg muscles with marked elevation of creatine kinase (CK) activity (20 to 30-fold) were the characteristic features. The parents of the patients had no symptoms or signs of myopathy. Their serum CK-activity was in the normal range. There was parental consanguinity, so the distal myopathy in these brothers is probably an autosomal recessive inheritance.

Adult↗
PubMed1981

Effects of sleep deprivation on the activity of selected metabolic enzymes in skeletal muscle.

The effect of 120-h sleep deprivation on the activity of selected enzymes of energy metabolism in skeletal muscle was studied in seven healthy volunteers. The results showed a significant decrease in the activity of malate dehydrogenase, citrate synthase, glycerol-3-phosphate dehydrogenase and lactate dehydrogenase. Triosephosphate dehydrogenase, hexokinase, and hydroxyacyl-CoA-dehydrogenase activities showed an insignificant decrease. The findings are indicative of (1) decreased aerobic oxidation capacity; (2) reduced function of reducing-equivalent carriers from cytosol across the mitochondrial membrane; (3) relative accentuation of the non-aerobic glycolytic pathway; (4) a prediabetic type of muscle metabolism.

3-Hydroxyacyl CoA Dehydrogenases↗
PubMed1980

Psychometric studies of pirenzepine.

A single dose of 10 mg of pirenzepine given i.m. to 7 healthy volunteers did not elicit local or general signs of intolerance within 6 hours after injection. We also found no significant differences in tests of vegetative symptomatology, visual motor coordination, attention concentration, critical flicker test threshold, nor any other aspects of the central nervous or cardiovascular systems. Ten patients with ulcers receiving pirenzepine 25 mg twice daily also showed no significant changes in psychometric tests, neurotic symptomatology, anxiety, depressive signs and clinical state during 4 weeks treatment. Side-effects of the anticholinergic type occurred only after high doses.

Adult↗
PubMed1980

Hodgkin's disease in the Waldeyer's ring.

Amongst 128 patients with Hodgkin's disease six were found to have involvement of Waldeyer's lymphoid ring, one in tonsil and five in nasopharynx. Of these, three were histologically verified by biopsies taken from Waldeyer's ring, whilst three had histology only from upper cervical lymph nodes. Disease was confined to head and neck area by five, one had a more widespread process. Discussing diagnostic difficulties of nasopharyngeal involvement the importance of roentgentomography is stressed. Principles of radiotherapy are expounded and a more diligent search for detecting nasopharyngeal disease is proposed.

Adult↗