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Biomedical subjects

E Kucharz

Publications and source records attributed to E Kucharz.

At least 37 records · Page 2Linked to original sources
PubMed1983

Aldehyde content of collagen from liver of rats with collagen-like syndrome.

Collagen was obtained from liver tissue of rats with hydralazine-induced collagen-like syndrome. Bacterial collagenase was used to solubilize the collagen, and the aldehyde content of this material was measured using N-methyl benzothiazolone hydrazone. The aldehyde content was decreased in the in the collagen from livers of rats with collagen-like syndrome. The changes in hepatic collagen may be analogous to the effect of aging on collagen, wherein collagen cross-linking is strengthened although qualitative changes in cross-linking may lower the measurable aldehydes.

Aldehydes↗
PubMed1983

Effect of anti-inflammatory steroids on the activity of selected lysosomal hydrolases in serum of rats with experimental liver fibrosis.

Liver fibrosis was produced in rats by subcutaneous injections of carbon tetrachloride. The effect of anti-inflammatory steroids (hydrocortisone, prednisone, dexamethasone) on the activity of serum lysosomal hydrolases (beta-N-acetylglucosaminidase, beta-glucosidase and alpha-mannosidase) was investigated in healthy and CCl4 poisoned rats. The marked increase of enzymatic activity in blood serum was observed in animals with CCl4-induced liver fibrosis. Quite opposite results were found in normal rats treated with glucocorticosteroids. In rats which were given both carbon tetrachloride and corticosteroids the obtained values of serum enzymatic activity did not have a uniform character as compared to controls or CCl4 intoxicated rats. Presumable mechanisms of the results obtained are discussed.

Animals↗
PubMed1982

Collagen content in tissues of irradiated rats.

Collagen content was measured in the skin, liver, lungs, kidneys and heart muscle of rats irradiated with a single dose of gamma rays (500 R). A decrease of total collagen content was found in the skin and was produced by a decrease of neural salt-soluble and acid-soluble collagen. A slight increase of insoluble collagen was observed in the skin, liver and lungs.

Animals↗
PubMed1982

Influence of anti-rheumatic drugs on the activity of collagenolytic cathepsin in hepatic culture.

A decrease of the collagenolytic cathepsin activity was found in foetal and adult hepatic slices cultured in vitro with acetylsalicylic acid, phenylbutazone, aminophenazone, indomethacin and naproxen. The most marked decrease was found in slices treated with naproxen and indomethacin. Acetylsalicylic acid produced only a slight diminution of the enzyme activity. Studies on hepatotoxicity indicated that acetylsalicylic acid and phenylbutazone are very toxic drugs, whereas the naproxen toxicity was very low.

Aminopyrine↗
PubMed1982

Collagen metabolism in patients with myocardial infarction.

The healing of myocardial infarction is accompanied by an increased accumulation of collagen in the heart muscle. Indexes of collagen metabolism (serum hydroxyproline, hydroxylysine and collagen-like protein concentration) were measured in 60 patients with myocardial infarction and 30 healthy blood donors. Determinations were carried out 1, 2, 3 days after admission and between the 10th-14th and 21st-25th day of the illness. It was found that hydroxyproline and hydroxylysine decreased in the first days of the disease, and subsequently increased in the course of the healing process accompanied by an increased collagen-like protein level. The changes did not depend on the total protein concentration.

Adult↗
PubMed1982

[Experimental evaluation of toxicity of selected mixtures used in metallurgy].

The investigation is aimed at evaluation of the effects of copper-coating and bonderizing solutions and oil--emulsion used in technological processes in the metallurgic industry upon hematological and biochemical changes in rats' peripheral blood. The studies were performed on male Wistar rats. The solutions were carried onto hairy and depilated skin. Acute toxicity was evaluated in two-weeks' experiment and chronic toxicity in three--months' experiment. The copper-coating solution was found to be most hazardous, whereas the oil emulsion-the least hazardous. The copper-coating and bonderizing solutions, when chronically applied, result in anaemia and changes in the percentage picture of leucocytes, and also increased blood concentration of uric acid, creatinine and hydroxyproline. No significant changes in concentrations of the remaining biochemical indices were found. The results point to synergistic effects of oil-emulsion and copper-coating solution.

Animals↗