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Biomedical subjects

E Kruse

Publications and source records attributed to E Kruse.

69 records · Page 4Linked to original sources
PubMed1988

Distribution and metabolism of estramustine in HeLa cells and the human prostatic tumour cell line 1013L.

The metabolism of estramustine [estradiol-3-N-bis(2-chloroethyl) carbamate] was investigated in the human prostatic tumour cell line 1013L and the human cervix tumour cell line HeLa S3. Uptake studies revealed that estramustine (EM), and its 17-ketoanalogue estromustine (EoM), differed in their nuclear binding pattern in 1013L cells but not in HeLa cells. Most of the nuclear radioactivity from both EM and EoM was found in the fraction containing the majority of the phospholipids. HPLC studies on EM-treated 1013L cells showed the presence of the oxidized metabolite EoM, in the medium, an enrichment of estradiol and estrone in whole cells and EM and EoM bound to the nuclear protein matrix. Similar studies on the HeLa cell line showed a completely different pattern, no metabolites other than EoM were found in the cell medium and whole cells but several very lipophilic metabolites were found bound to the nuclear protein matrix. On investigation of other tumour cell lines these metabolites were found to be unique to HeLa cells. The results extend our knowledge concerning EM and demonstrate that the cell line 1013L is a relevant model system for studying drugs active against human prostatic tumours.

Cell Line↗
PubMed1986

Nuclear protein matrix as a target for estramustine-induced cell death.

The effect of estramustine [estradiol 3-N-bis(2-chloroethyl)carbamate] on the human prostatic tumor cell line 1013L was investigated. Cell proliferation experiments revealed that estramustine cytotoxicity varied during the different phases of cell growth. Maximum cell killing was found in early log phase, but cell death also occurred in the stationary phase. Mitotic arrest was found at cytotoxic concentrations throughout the log phase. Subcellular distribution studies showed that the cellular uptake of estramustine increased throughout the log phase and remained steady during the stationary phase. Nuclear uptake in contrast was similar in all phases, whereas a preferential binding to the nuclear protein matrix was found to increase throughout the log phase and even during the stationary phase of growth. This implicates the nuclear protein matrix as a target for estramustine cytotoxicity.

Cell Line↗
PubMed1985

[What average hearing gains can be expected at present with children wearing hearing aids?].

The narrow band noise-audiometric free-field hearing threshold of hearing aids encloses at least some of the essential part of the acoustic language field even in marked unisensory infant hearing impairment. Thus auditive promotion is to be preferred even in "deaf" children compared to optic-tactile aids. This has an advantage for language development and also has consequences for the method of training. Statistical evaluation of the pure tone and narrow band noise-audiometric data allows conclusions to be drawn about the quality of hearing aids adjustment and especially about the importance of the medium pure tone-audiometric hearing thresholds limit at 70-75 dB.

Audiometry↗
PubMed1984

[Electromyography in function disorders of the cricothyroid muscle following trauma and strumectomy].

32 patients with a disturbed function of the cricothyroid muscle were examined electromyographically and some also electroneurographically. Thyroid surgery had preceded in 22 patients and a traumatic lesion in 10 patients. Clinical observation showed that all patients suffered from an instability and lowering of the speaking voice as well as a reduction of vocal range. This was caused by a deficient tension of the vocal cords due to an insufficiently innervated cricothyroid muscle. In healthy subjects a continuous muscle activity of the cricothyroid muscle can be noted even in a resting position. During phonation the density and amplitude of this activity increases, showing a spindle-shaped pattern. In our strumectomised patients the resting activity of the impaired side was either greatly diminished when compared with the healthy side, or showed signs of denervation including the recording of fibrillations and positive sharp waves. The activity during phonation was reduced or completely missing. Electrical stimulation of the superior laryngeal nerve produced a delayed indirect muscle response. In those patients with traumatic lesions, the resting activity was reduced while a regular activity increase during phonation was completely missing. However, no signs of denervation were found in these patients. Our findings show evidence of neurogenic damage during thyroid surgery and an impairment of structures of the muscle itself following traumatic lesions.

Adult↗
PubMed1980

[Computer aerography. Methodology and clinical use exemplified by labio-maxilla-palate clefts (author's transl)].

Computer aerography, which is based on the use of the double-aerometer by Sven Smith, is described as an objective method for clinic-scientific problems. Medical examination of labio-maxilla-palate-cleft patients shows how this procedure, which has a low degree of inconvenience and is also applicable to children, contributes essentially to the settling of clinic-scientific problems. Further scopes are still being tested.

Cleft Lip↗
PubMed1978

Effect of theophylline on myocardial adenylate cyclase activity.

Theophylline (0.001-10.0 mM) did not increase but rather decrased adenylate cyclase activity (AC) of guinea-pig auricles. Isoprenaline (1-100 microgram) and sodium fluoride (0.3-10.0 mM) stimulated AC in a concentration-dependent manner.

Adenylyl Cyclase Inhibitors↗
PubMed1978

[Phoniatric therapeutical possibilities for paralysis of the vocal lips in paramedian position following a strumectomy (author's transl)].

The phoniatric-logopedic therapeutical possibilities and results of the two most frequent causes of paralysis following a Strumectomy are presented here. The major aspect of this treatment is the application of electric therapy. Its correct usage will be explained. This "conservative" therapy should precede every operative measure and should follow again once the wound has healed. A new post-operative phoniatric therapy method following Glottis widening surgery will also be described. This therapy is based upon the functioning of the Ventricularis muscle, which operates independent of the Recurrens nerve. Furthermore, this therapy functions independent of the operation methods and leaves the natural vocal cord phonation intact, despite vocal cord paralysis.

Electric Stimulation↗
PubMed1975

[Ventricular cord hyperplasias of the larynx-pathogenesis and treatment (author's transl)].

Primary hyperplasia of the ventricular cords can produce voice disturbance or can be a secondary change caused by a primary voice disorder. Primary hyperplasia, besides inflammation or tumour, arises in aging people by a metaplastic process of the glandular epithelium of the ventricular cords. These lesions are called "grandular-cystic ventricular cord hyperplasias". Secondary hyperplasias are mainly of a fibrous nature. These "fibrous-vicarious hyperplasias" arise in hyperkinetic functional voice disorders, in pareses or in some cases of extensive chronic hyperplastic or oedematous laryngitis if the vocal cords can no more be used for normal phonation. Endolaryngeal microsurgical excision from the ventricular cords followed by voice exercises enables us to treat the "ventricular cord voice" successfully within a short time.

Aging↗
PubMedDate not supplied

Reperfusion of focal ischemia of varying duration: postischemic hyper- and hypo-perfusion.

Reperfusion into focal ischemia was studied in 25 cats after middle cerebral artery (mca) occlusion of 15 min to 2 hours duration. Changes in cerebral blood flow (CBF) were followed with the hydrogen clearance method in the center and periphery of the ischemic lesion expected. Postischemic hyperperfusion was found often after 15 and 30 min ischemia and regularly after 60 min mca occlusion. It was followed by normal flow after 15 and 30 min occlusion and by postischemic hypoperfusion after 1 hour ischemia. After 2 hours occlusion hypoperfusion generally was not preceded by hyperperfusion. After 60 min ischemia hyperperfusion could not prevent the development of severe hypoperfusion, but often was accompanied by a marked flow reduction in the periphery of the mca territory. The data indicate that hyperperfusion after ischemic periods lasting 60 min and more induces hypoperfusion in the area itself and in neighbouring regions by affecting perfusion pressure and thereby may enlarge ischemic damage.

Animals↗
PubMedDate not supplied

Uptake and metabolism of estramustine in the Dunning R3327H tumour.

A time-dependent plasma, tumour cell and nuclear uptake was found after intraperitoneal 3H-estramustine administration, in rats bearing the Dunning H tumour. Uptake of the drug into tumour cells had not reached a maximum after 4 h, whereas no real change was seen in the nuclear fraction after 2 h. Binding to the nuclear protein matrix of Dunning H tumour cells of 3H-estramustine was also found (2.46 pmol/g protein). High performance liquid chromatography demonstrated the existence of estramustine, its oxidized metabolite estromustine, estradiol and estrone in the plasma and the tumour after intravenous administration of 100 microCi 3H-estramustine.

Animals↗