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E Kruse

Publications and source records attributed to E Kruse.

At least 55 records · Page 3Linked to original sources
PubMed1995

Reduction of coproporphyrinogen oxidase level by antisense RNA synthesis leads to deregulated gene expression of plastid proteins and affects the oxidative defense system.

A full-length cDNA sequence encoding coproporphyrinogen oxidase was inserted in inverse orientation behind a CaMV promoter and transferred to tobacco (Nicotiana tabacum) by standard transformation techniques. Transformants showed reduced coproporphyrinogen oxidase activity and accumulation of photosensitive coproporphyrin(ogen), indicating antisense RNA expression. An inverse correlation was observed between the level of coproporphyrinogen oxidase and transformant phenotype. The latter is characterized by a broad range of growth retardation and necrosis, indicating oxidative leaf damage. Coproporphyrinogen is an apparent chromophore and its excitation finally leads to the production of reactive oxygen. Evidence is presented that indicates a direct correlation between the accumulation of non-metabolized coproporphyrinogen and oxidative damage to cellular structural components. Enzymatic and non-enzymatic antioxidants were investigated. Whereas superoxide dismutase activity increased in transgenic plants, catalase and ascorbate peroxidase activity remained constant. Tocopherol, rather than carotene or zeaxanthin, seemed to be involved in detoxification, indicating the putative localization and allocation of coproporphyrinogen. Expression of coproporphyrinogen oxidase antisense RNA did not significantly influence the level of other enzymes in the chlorophyll metabolic pathway, but deregulated gene expression of nuclear encoded plastid proteins. Accumulation of coproporphyrinogen and/or the resulting effects, such as oxidative stress, impairs a plastid/nuclear signal which may adapt gene expression to the plastid state.

Antioxidants↗
PubMed1995

Isolation, sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley.

We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other organisms. We propose that tobacco UROD has an N-terminal extension of 39 amino acid residues. This extension is most likely a chloroplast transit sequence. The in vitro translation product of UROD was imported into pea chloroplasts and processed to ca. 39 kDa. A truncated cDNA, from which the putative transit peptide had been deleted, was used to over-express the mature UROD in Escherichia coli. Purified protein showed UROD activity, thus providing an adequate source for subsequent enzymatic characterization and inhibition studies. Expression of UROD was investigated by northern and western blot analysis during greening of etiolated barley seedlings, and in segments of barley primary leaves grown under day/night cycles. The amount of RNA and protein increased during illumination. Maximum UROD-RNA levels were detected in the basal segments relative to the top of the leaf.

Amino Acid Sequence↗
PubMed1995

Sociodemographic variables of a German sample of patients with contact granuloma.

We report 28 German patients with contact granuloma (27 male, 1 female). Their mean age was 52 years (ranging from 35 to 70). Thirty-two percent were retired. The occupations of the others represented a wide range of different jobs. The majority of the sample had a middle educational level. Most patients lived with their family or with a partner. According to self-assessments, 68% had average daily strain on their speaking voice. All patients were nonsmokers. The patients felt themselves more disturbed by somatic troubles as the general population. Heartburn was felt by nearly half of the patients. A little more than half of the patients suffered from globus sensation. Thus, it is not possible at present to explain the laryngeal contact granuloma by sociodemographic data, vocal stress, or special somatic complaints in this sample. Therefore a multifactorial etiology should be supposed.

Adult↗
PubMed1995

[Early detection and hearing aid management of pediatric unilateral hearing loss].

BACKGROUND: Unilateral hearing loss in children is frequently an incidental diagnosis, usually late. As in binaural hearing disorders, early detection is extremely important to protect the function of the "remaining ear" and thus ensure acquisition of speech. Prompt initial probatory treatment with a conventional hearing aid is necessary to take advantage of the sensitive phases of development and maturation of the auditory pathways. This allows lateralisation as well as a better discrimination, avoiding cocktail party and non-physiologic head shadow effects. PATIENTS: The study includes 32 children with a conventional hearing aid. The average age of diagnosis was 5.5 years. The children were initially treated with a conventional hearing aid. RESULTS: The etiology remained unknown in the majority of the cases. Twenty-seven of the children were observed over a longer period, averaging 2 years. Hearing aid acceptance was found to be 63% with a measurable hearing improvement in 59% of the patients, even in the presence of severe hearing disorders. In the majority of the cases, the etiology remained unknown. CONCLUSION: More knowledge about risk factors for unilateral hearing losses in children should improve the chances for early detection, making hearing aid supply at a younger age possible. This should happen firstly in a probatory manner and in cases with hearing aid acceptance and a measurable hearing improvement definitely.

Child↗
PubMed1995

["The main cause of the differences is the crime": An attempt at explaining the excess mortality of illegitimate children in The Hague in the mid-nineteenth century].

During the last quarter of the 19th century, illegitimate children had much higher infant mortality than legitimate children. To investigate which factors played a role in this, we collected information on the household situation and the life course of all illegitimate children, born in the Dutch city of The Hague in the years 1850-1852. Use was made of birth and death certificates and of the population registers of the city. We compared our information with data on a random sample of legitimate children,, born in the same city. After children had reached the age of one month, mortality among illegitimate children became very high, compared to that of our reference group. Proportional hazards analysis showed that this excess mortality was caused by a set of factors, the most important being the age of the mother at birth, regional background, and the socio-economic group to which the notifier of the birth belonged. Aside from those factors, legal status of the child as such had an effect on infant mortality. The unwed mothers most likely to lose their child in its first year of life lived alone with their child and were obliged to resume work to support themselves and their child. The opportunity to breastfeed may have played a key role in this.

Cause of Death↗
PubMed1995

Coproporphyrinogen III oxidase from barley and tobacco--sequence analysis and initial expression studies.

Coproporphyrinogen III oxidase (coprogen oxidase; EC 1.3.3.3) is part of the pathway from 5-amino-levulinate to protoporphyrin IX which is common in all organisms and catalyses oxidative decarboxylation at two tetrapyrrole side chains. We cloned and sequenced full-length cDNAs encoding coprogen oxidase from barley (Hordeum vulgare L.) and tobacco (Nicotiana tabacum L.). They code for precursor peptides of 43.6 kDa and 44.9 kDa, respectively. Import into pea plastids resulted in a processed tobacco protein of approx. 39 kDa, which accumulated in the stroma fraction. Induction of synthesis of recombinant putative tobacco mature coprogen oxidase consisting of 338 amino-acid residues in Escherichia coli at 20 degrees C result in a catalytically active protein of approx. 39 kDa, while induction of its formation at 37 degrees C immediately terminated bacterial growth, possibly due to toxic effects on the metabolic balance of tetrapyrrole biosynthesis. The plant coprogen oxidase gene was expressed to different extents in all tissues investigated. This is most likely due to the differing requirements for tetrapyrroles in different organs. The steady-state level of mRNA did not significantly differ in etiolated and greening barley leaves. The content of coprogen oxidase RNA reached its maximum in developing cells and decreased drastically when cells were completely differentiated. Functioning of the two photosystems apparatus requires the synthesis of all pigment and protein components during plant development. It is speculated that the enzymes involved in tetrapyrrole synthesis are developmentally rather than light-dependently regulated. Regulation of these enzymes also guarantees a constant flux of metabolic intermediates and avoids photodynamic damage by accumulating porphyrins.

Amino Acid Sequence↗
PubMed1995

[Expressive vocabulary: comparison of 2 psychological test procedures in kindergarten children].

Fifty-five subjects were drawn from a population of average kindergarden children. Their ages ranged between 3; 4 and 4; 11 years. Two vocabulary tests were administered individually in a quiet room to each subject. A new instrument for testing the active vocabulary (Subtest "vocabulary" of the K-ABC, 1991) was compared with an established one (AWST 3-6, 1979) to see whether they agree sufficiently for the new to replace the old. Raw scores were computed according to the test instructions for each correct response and transformed into normscores. These were compared, and both tests correlated with r = 0.69 (p = 0.0001). So, the K-ABC-Subtest "vocabulary" may be administered in the first diagnostic setting. The mean of the differences of both vocabulary tests is 0.1; yet, in some cases large differences between the results of both vocabulary tests are to be expected, because the differences vary in the broad range from -15.5 to +15.7 (= +/- 2 SD). Therefore the more detailed AWST 3-6 should be used to assess the amount of active vocabulary in children with a low norm score in the K-ABC-Subtest "vocabulary".

Child, Preschool↗
PubMed1994

[Laryngeal contact granuloma--a psychosomatic disorder?].

In a multidimensional design using the hypothesis of a psychosomatic genesis, 28 patients (1 female, 27 male) showed, in their personality profile, increased scores in 'impulsiveness', 'emotionality (neuroticism)', 'strain' and 'inhibition'. In addition to the voice disorders other somatic symptoms were observed, especially general symptoms as dysthymia, depression and excessive vegetative stimulation. Their social competence was reduced by the fear of making contacts and the inability 'to say no'. Also psychosocial stress factors were registered due to loss of a loved one with feelings of an existential danger. Thus, contact granuloma can be considered as a secondary reaction to a specific combination of personal disposition and social life situation. Lastly a causal model is presented, which can be used as a diagnostic criterion.

Adult↗
PubMed1993

Integration of a cyanobacterial protein involved in nitrate reduction (narB) into isolated Synechococcus but not into pea thylakoid membranes.

Chimeric genes comprised of Rubisco small subunit transit peptide fused in frame with full-length and truncated sequences of a nitrate reductase (narB) structural gene of Synechococcus were constructed. Fusion proteins were synthesized in a rabbit reticulocyte system. In thylakoido integration of synthetic proteins resulted in the association of the full-length narB-coded protein to the Synechococcus photosynthetic membranes. The membrane-associated protein was sensitive to trypsin treatment but could not be removed by washing in the presence of NaBr. Trypsin pretreatment of thylakoids abolished the capability for association. The association of the narB-coded protein with thylakoids might require another membrane protein whose identity is not known. It is proposed that the Synechococcus narB polypeptide is a peripheral, membrane bound protein anchored to the thylakoids via a short hydrophobic domain while the major part of the protein resides on the outer side of the thylakoid membranes. The chimeric narB proteins were processed and imported by intact pea chloroplasts in vitro; however, the mature proteins were found localized in the stroma and not in the thylakoid membrane fraction. Similarly, the attempt to integrate the protein in vitro into isolated pea thylakoid membranes failed although these membranes incorporate early light-inducible proteins.

Animals↗
PubMed1993

Expression of heat shock proteins during development of barley.

Barley heat shock proteins have been cloned, characterized by hybrid release translation and sequenced. Clones coding for proteins of 17, 18, 30, 32 and 70 kDa have been obtained. Out of these the 32 and 30 kDa proteins have been characterized as precursors to plastidic proteins of 26 kDa by posttranslational transport and by cDNA sequencing. The coding regions of these two transcribed genes are highly homologous. Accumulation of the plastid HSP as well as of HSP 70 as well as their corresponding mRNAs has been studied in 2- to 6-day old seedlings and in the 7-day old barley leaf. The mRNA for all investigated proteins were only found after a heat shock; the mRNA levels increase towards the tip of the leaf and with development. Furthermore, under the conditions used the mRNAs for all investigated heat shock proteins accumulate in parallel. Unexpectedly, both proteins, HSP 70 and HSP 26, are found by western blotting in the 2-day old control plants in the absence of any inducing heat shock. At later stages of development and in the leaf gradient only immunoreactivity with HSP 70 was observed. In contrast to the levels of their mRNAs the highest levels of HSP 30-26 and 70 have been observed in the basal segments indicating that translational control plays a role during HSP expression. Under severe heat shock a protein of 30 kDa is induced whose identity is not known but which reacts with the antibody to HSP 30-26 and might represent the accumulating precursors of the plastidic proteins.

Amino Acid Sequence↗
PubMed1992

Integration of early light-inducible proteins into isolated thylakoid membranes.

An in-vitro system has been established to study the integration of early light-inducible proteins (ELIP) into isolated thylakoid membranes. The in-vitro-expressed ELIP precursor proteins exist in two forms, a high-molecular-mass aggregate which is accessible to trypsin but no longer to the stromal processing protease and a soluble form which is readily cleaved to the mature form by the stromal protease. The mature form of ELIP is integrated into thylakoid membranes; its correct integration can be deduced from the observation that the posttranslationally transported products and the in-vitro integrated ELIP species are cleaved by trypsin to products of the same apparent molecular mass. Trypsin-resistant fragments of high-molecular-mass and low-molecular-mass ELIP appear to have the same size. The processed ELIP species, as well as an engineered mature form of ELIP, are integrated into isolated thylakoid membranes. Integration of the mature protein occurs in the absence of stroma, into sodium-chloride-washed, and trypsin-treated thylakoid membranes. The process of integration is almost temperature independent over 0-30 degrees C. Analysis of the time course of integration leads to the conclusion that, under in-vitro conditions, processing but not integration into membranes is the rate-limiting step. In the absence of stroma, the ELIP precursor is bound to the thylakoid membranes, however, it is no longer accessible to the stromal maturating protease when added after binding has occurred. In conclusion, integration of ELIP differs in many essential details from that of its relatives, the light-harvesting chlorophyll a/b protein family.

Chloroplasts↗
PubMed1990

[ECG in acute myocardial infarct. Continuous registration in thrombolytic therapy].

For 35 patients with acute myocardial infarction undergoing thrombolytic therapy (31 patients with streptokinase and 4 with rt-PA), the treatment effect was assessed angiographically and correlated with ECG changes. These ECG changes were found by continuous registration of eight "strategically" located precordial leads in patients with anterior myocardial infarction, and five precordial leads plus three limb leads in those with posterior myocardial infarction. In 31 patients (group A), successful reperfusion could be verified by angiography, while in 4 patients the infarct-related vessel remained closed. In 3 further cases (group B), recanalization could be achieved only by additional mechanical intervention. The average duration of ECG surveillance was 87 min in group A and 85 min in group B. A significant change of R-amplitudes during this period of time could not be observed. ST-60-value of group A (total of ST-elevations and depressions 60 msec after S of all surveillance electrodes, divided by number of electrodes) was reduced under thrombolysis from 0.377 mV (SD +/- 0.270) to 0.133 mV (SD +/- 0.118) (p less than 0.004). This means a reduction of 64%, while the corresponding values in group B did not change (0.535 mV, respectively 0.546 mV) before and after therapy. When only the electrode with the highest ST-60-value was checked before therapy ("sensitive lead"), reduction in group A was as high as 71%. Except for two patients, all other 29 patients with successful reperfusion either precordially or in the limb leads showed a reduction of ST-segment abnormalities (ST 60) of more than 50% during surveillance time. This could not be observed in any of the unsuccessfully treated patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

Identification by C-banding of two human prostate tumour cell lines, 1013L and DU 145.

Two human prostate carcinoma cell lines are easily distinguished by C-banding. DU 145 has one metacentric chromosome with a large centromeric band, 1 metacentric and 2 acrocentric chromosomes with interstitial C-bands. The Y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in 1013L only one metacentric chromosome contained distinct extra C bands--one at the centromere and one interstitially. The Y chromosome was present in 72% of the cells at passage 15 and in 20% at passage 22, but by passage 44 it had disappeared. Karyotype analysis using G-banding revealed that DU 145 had retained several of its original markers, and demonstrated multiple marker chromosomes in 1013L.

Chromosome Aberrations↗
PubMed1989

Transiently expressed early light-inducible thylakoid proteins share transmembrane domains with light-harvesting chlorophyll binding proteins.

The early light-inducible proteins (ELIPs) of barley chloroplasts are encoded in two multigene families yielding end products of different molecular mass. Sequencing of complete cDNA clones showed that the low and high molecular mass proteins differ by the presence or absence of a 65 amino acid peptide in the amino-terminal part of the mature proteins. Two domains of the ELIPs reveal striking similarity in amino acid sequence with two transmembrane domains of all known light-harvesting chlorophyll a/b-binding proteins from photosystem I and II and may be of importance in anchoring the polypeptides in the membrane. The cDNA sequences of two low molecular mass ELIPs differ by an insert of 5 codons in the putative transit peptide. By in vitro transcription and translation of the cloned DNA and subsequent transport of the products into chloroplasts it could be established that the precursors are processed into products of identical apparent molecular mass. In vitro translated ELIPs were incorporated into thylakoid membranes both as precursors and mature polypeptides. It is suggested that ELIPs are pigment-free substitutes for light-harvesting polypeptides in the assembly of photosynthetic units during early development of thylakoids.

Amino Acid Sequence↗
PubMed1989

The effect of estramustine, nor-nitrogen mustard and tauromustine on macromolecular labelling in the human prostatic tumour cell line 1013L.

To further clarify the mode of action of estramustine, the influence on macromolecular synthesis in the human prostatic tumour cell line 1013L was investigated. Cell treatment with estramustine, nor-nitrogen mustard and tauromustine, followed by radioactive nucleotide and leucine incorporations, as a measure of RNA, DNA and protein labelling, were carried out. The initial effect of estramustine clearly differed from that obtained after treatment with nor-nitrogen mustard and tauromustine. No inhibition of DNA synthesis was found whereas an inhibition of overall RNA synthesis was predominant. Adaption of an established RNA separation method was used in an indepth study of RNA labelling after estramustine treatment. An inhibition of 29S, 18S and 4-7S RNA was found after estramustine treatment, indicating disturbances in either RNA processing or RNA transport. The lack of 45S RNA labelling additionally indicates pre-ribosomal inhibition.

Affinity Labels↗
PubMed1989

[Differential diagnosis of functional voice disorders].

In phoniatrics the differential diagnosis of functional voice disorders is mainly based on the medical history of the patient, laryngoscopic and stroboscopic findings. On the one hand the typical laryngoscopic picture must be stressed, on the other hand the central significance of stroboscopy for the functional diagnosis of the larynx has to be mentioned. Only the detailed assessment of the stroboscopic signs and symptoms described by Schönhärl allows a therapy-relevant differentiation between cause-related diagnosis and compensatory secondary symptoms with a necessary distinction of psychosomatic disorders.

Diagnosis, Differential↗