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Biomedical subjects

E Honda

Publications and source records attributed to E Honda.

At least 37 records · Page 2Linked to original sources
PubMed2000

Capillary zone electrophoresis of albumin-depleted human serum using a linear polyacrylamide-coated capillary: separation of serum alpha-and beta-globulins into individual components.

The separation of human serum globulins into individual components was investigated by capillary zone electrophoresis (CZE) using a linear polyacrylamide-coated capillary at pH 7.4. Prior to CZE analysis of globulin components present in serum, it was found that it was necessary to remove albumin. Preparation of albumin-depleted human serum with a HiTrap Blue column allowed the detection of alpha- and beta-globulin components as a series of peaks. Almost all the peaks, both narrow and broad, observed in CZE analysis could be assigned to six globulin components (alpha1-acid-glycoprotein, alpha1 -antitrypsin, haptoglobin, alpha2-macroglobulin, Gc-globulin, and transferrin) by using the technique of antibody-based indirect detection. The CZE results, obtained from serum preparations from three healthy adults and six patients, showed that the CZE system might be capable of detecting qualitative differences among individuals with regard to individual globulin components.

Acrylic Resins↗
PubMed2000

Perception of temporal properties in self-generated songs by Bengalese finches (Lonchura striata var. domestica).

In the songbird forebrain, neuronal selectivity for temporal properties of each bird's self-generated song has been well described, but the behavioral and perceptual correlates of this selectivity are not known. By operant procedures, the authors trained Bengalese finches (Lonchura striata var. domestica) to discriminate between songs that were played normally and in reverse. Male Bengalese finches learned the discrimination quicker when their self-generated song was used as a stimulus than when a song of another conspecific bird was used. When the global note order was retained but each note was locally reversed, the song was more likely to be regarded as a forward song by the singer himself, but not by other birds. These results provide psychophysical evidence that the special processing of the self-generated song observed at the neural level might reflect an individual's perception of his self-produced song.

Analysis of Variance↗
PubMed2000

Effects of head and body position on two- and three-dimensional configurations of the upper airway.

This study was carried out to test the hypothesis that changes in head/body position induce changes in upper-airway dimensions. Contiguous images were obtained by means of magnetic resonance imaging in normal awake subjects during nasal breathing. A statistical analysis was made on 5 consecutive slices, including the most constricted sites in both the retropalatal and retroglossal regions. Dimensional changes in the upper airway in association with changes in head/body position were evaluated. In the retropalatal region, there was a significant decrease in the lateral dimension in the lateral recumbent position compared with that in the supine position. The cross-sectional area in the retroglossal region was significantly increased in both the "supine with the head rotated" and "lateral recumbent" positions. This change was accompanied by significant volumetric changes in the retroglossal region. Thus, sleeping with the head rotated may be effective for improving upper-airway obstruction.

Adult↗
PubMed2000

Human immune response to sand fly salivary gland antigens: a useful epidemiological marker?

Antibody (IgG) responses to salivary gland homogenate and to a recombinant salivary protein from the sand fly Lutzomyia longipalpis were investigated using sera from children living in an endemic area of visceral leishmaniasis in Brazil. We classified children into four groups according to their responses to Leishmania antigen: (Group I) positive serology and positive delayed type hypersensitivity (DTH), (Group II) positive serology and negative DTH, (Group III) negative serology and positive DTH, and (Group IV) negative serology and negative DTH. A highly significant correlation was found between anti-salivary gland IgG levels and DTH responses. An L. longipalpis salivary recombinant protein used as an antigen in an enzyme-linked immuno sorbent assay (ELISA) gave a significant but different result. A positive correlation was found between anti-Leishmania IgG and anti-recombinant protein IgG titers. The results indicate that sand fly salivary proteins may be of relevance to the study the epidemiology of leishmaniasis.

Animals↗
PubMed1999

Metallic residues as a source of artifacts in magnetic resonance imaging of the temporomandibular joint.

OBJECTIVES: To establish the source of metallic artifacts on postoperative MRI of the temporomandibular joint after arthroplasty. METHODS: An experiment was carried out to evaluate MR artifacts which were thought to have been caused by minute metallic particles deposited at surgery. Four types of cutting instruments were used to cut an extracted tooth: a diamond bur attached to a dental air turbine handpiece, a steel bur and bone bur attached to a dental micromotor handpiece and a bone file. The tooth fragments were collected in plastic centrifuge tubes in water. These samples were evaluated with six MR sequences. RESULTS: MR artifacts were clearly demonstrated in samples using the bone bur and the bone file but not the diamond and steel burs. CONCLUSIONS: The artifacts seen on postoperative MRI were derived from minute metal particles from the bone bur or file used for condylar arthroplasty. Metal artifacts should be considered when interpreting postoperative MR images.

Adult↗
PubMed1998

Cerebrospinal fluid ferritin in glioblastoma: evidence for tumor synthesis.

Ferritin concentrations in cerebrospinal fluid (CSF) of 16 patients with glioblastoma were found to be very high (mean, 103.0 ng/mL) relative to 29 patients with viral meningitis and 20 patients with headache (mean concentrations, 5.4 and 4.3 ng/mL respectively). Simultaneous measurement of ferritin in CSF and serum revealed CSF concentrations exceeding those in serum in 11 of 16 patients with glioblastoma (CSF vs. serum ratio, 132.6%), contrasting with very low ratios in patients with meningitis (7.9%) or headache (4.9%). Ferritin was detected immunocytochemically in tumor cell cytoplasm in a resected glioblastoma. Thus, ferritin in CSF appears to be produced by glioblastoma cells, with a biologic significance requiring further investigation.

Adult↗
PubMed1998

Glutamatergic synaptic inputs to mouse supraoptic neurons in calcium-free medium in vitro.

The effects of Ca2+-free perfusion medium on excitatory postsynaptic currents (EPSCs) and potentials (EPSPs) were studied by whole-cell recordings from neurons of the supraoptic nucleus (SON) in trimmed slice preparations of mouse hypothalamus. EPSCs evoked with either focal stimulation to the SON or perfusion of slices with high K+-medium, spontaneous EPSCs (sEPSCs) and miniature EPSCs (mEPSCs) recorded from neurons of the SON were blocked by the glutamate receptor antagonist kynurenic acid (1 mM). While EPSCs evoked by focal stimulation were abolished in the presence of Ca2+-free perfusion medium; sEPSCs and mEPSCs remained. Neither the frequency nor the amplitude of the sEPSCs and mEPSCs significantly changed during the application of Ca2+-free perfusion medium. Perfusion of slices with high K+-medium increased the mEPSC frequency compared with that recorded in normal Ca2+-containing perfusion medium. In contrast, mEPSC frequency did not change during perfusion with Ca2+-free high K+-medium. In current-clamp mode sEPSPs were observed during the perfusion with Ca2+-free medium. Some sEPSPs recorded in Ca2+-free medium were sufficiently large to evoke action potentials. These results imply that spontaneous glutamatergic synaptic inputs to the hypothalamic neurosecretory cells exist in Ca2+-free perfusion medium. Thus, the present study suggests that Ca2+-free medium does not always block the synaptic transmission in hypothalamic slice preparations.

Action Potentials↗
PubMed1998

Nerve growth factor modulates Fc gamma receptor expression on murine macrophage J774A.1 cells.

We assessed the effect of nerve growth factor (NGF) on Fcgamma receptor (FcgammaR) expression on murine monocyte-macrophage J774A.1 cells. Flow cytometric analysis showed that treatment with various doses of NGF led to a rapid increase of FcgammaRI and FcgammaRII/III expression in a dose-dependent manner. Northern blot analysis with digoxigenin-labeled oligonucleotide probes demonstrated that NGF induced a marked increase in mRNA synthesis of FcgammaRI and FcgammaRII, but not FcgammaRIII. Since pretreatment with K-252a, a tyrosine kinase inhibitor, inhibited the stimulatory effect of NGF completely, the FcgammaR expression augmented by NGF may be mediated through p140trk with a tyrosine kinase domain. These results suggest that NGF may be a novel cytokine that is able to increase FcgammaRI and FcgammaRII expression on macrophages.

Animals↗
PubMed1998

Symptomatic spinal arachnoid cyst triggered by seat belt injury--case report.

A 49-year-old male presented with a symptomatic thoracic intradural arachnoid cyst triggered by seat belt trauma, which appeared within 3 weeks following a traffic accident. The initial symptom was a dull back pain induced immediately after the accident. Three and half months later, magnetic resonance (MR) imaging diagnosed the cystic nature of the lesion consistent with a low intensity on T1-weighted image which extended dorsally between the T-4 and T-6 levels. One month later, MR imaging showed that this cystic mass had enlarged in the anteroposterior direction associated with developing clinical symptoms. The final diagnosis of arachnoid cyst was made based on surgical findings and histological examinations. The spinal arachnoid cyst was probably a congenital arachnoid cyst or diverticula originating from the septum posticum which became enlarged due to sudden increase of intra-abdominal pressure caused by the fastened seat belt during the accident.

Accidents, Traffic↗
PubMed1997

Adaptation of equine herpesvirus 1 to unnatural host led to mutation of the gC resulting in increased susceptibility of the virus to heparin.

Heparin extensively inhibited infection of MDBK cells by equine herpesvirus 1 (EHV-1) strains adapted to bovine cells or hamsters, while the reagent merely reduced infectivity of strains passaged only in equine cells. The gC of two strains adapted to non-equine cells seemed to have higher affinity for heparin, although the reagent bound to both the gC and gB of all strains tested. Amino acid substitutions of the gC of the EHV-1 strains adapted to non-equine cells converged on the hydrophilic regions, amino acid residues 92 to 175, resulting in the glycoprotein becoming more cationic. These results indicate that these hydrophilic regions of the gC may be responsible for binding to heparin.

Adaptation, Physiological↗
PubMed1997

Simultaneous multilayer arthrotomography of the temporomandibular joint using photostimulable phosphor computed radiography.

OBJECTIVE: To compare the image quality of photostimulable phosphor computed radiography (CR) with that of a conventional screen-film system for simultaneous multilayer arthrotomography of the temporomandibular joint (TMJ). METHODS: X-ray attenuation, resolution and granularity was compared. Simultaneous multilayer tomography of TMJ was performed using a skull phantom and diagnostic quality of bone structures estimated. The clinical outcome is demonstrated in two typical cases. RESULTS: X-ray attenuation was larger with CR. Granularity was more prominent in edge-enhanced CR images and was associated with inferior diagnostic quality. However, it was improved by using a higher tube voltage. Clinical CR without edge enhancement showed a similar resolution to the conventional system but this decreased with enhancement. In an enhanced arthrotomogram, the boundary between contrast medium, bone and soft tissue were clearly defined and a fibrous adhesion also easily seen. CONCLUSION: Photostimulable phosphor CR is an acceptable alternative for simultaneous multilayer arthrotomography of the TMJ.

Adult↗
PubMed1997

Osmotic modulation in glutamatergic excitatory synaptic inputs to neurons in the supraoptic nucleus of rat hypothalamus in vitro.

To clarify influence of osmotic stimulation on the excitatory synaptic inputs to the neurosecretory cells of the supraoptic nucleus (SON), the blind patch technique was used in rat hypothalamic slice preparations. Stable whole-cell recordings were made from 22 neurons in the SON. To observe spontaneous excitatory postsynaptic currents (sEPSCs) in the SON neurons, membrane potentials were clamped between -50 and -90mV. The effects of hypertonic stimulation on the frequency of the sEPSCs were tested in 18 SON neurons. Bath application of mannitol 30 or 60 mM increased the frequency of the sEPSCs. During the application of mannitol (60 mM), the frequency of the sEPSCs increased in 12 of 15 neurons without a change in amplitude. Hypertonic stimulation with NaCl (30 mM) had similar effects to that of mannitol. The increased frequency of miniature EPSCs (mEPSCs) during mannitol application persisted in the presence of TTX in all 8 SON neurons tested with no change in amplitude. Both the non-NMDA antagonist CNQX at 10-30 microM (n = 6) and the non-selective glutamate antagonist kynurenic acid at 1 mM (n = 3) almost completely blocked the EPSCs while the NMDA antagonist AP-5 at 10 microM had no effect on the frequency of the EPSCs in the 4 neurons tested. During application of CNQX, mannitol (60 mM) was added to the perfusion medium in 3 SON neurons. Under these conditions, mannitol had no effect on the frequency of EPSCs. We conclude that hypertonic stimulation directly influences glutamatergic inputs to the neurosecretory cells of the SON by an action on the presynaptic terminals and enhances the excitatory synaptic events.

6-Cyano-7-nitroquinoxaline-2,3-dione↗
PubMed1996

Arthrotomographic sign of a 'double line' as an indicator of disc folding and sideways disc displacement in the temporomandibular joint.

Dual-space, single-contrast arthrotomolgraphy of the TMJ was performed on a 19-year-old woman with a history of pain and clicking. Simultaneous multi-layer arthrotomography with computed radiography was used for the present examination. Anterior disc displacement with reduction was observed in the sagittal plane, together with a double line in the lower joint space. The double line was interpreted as disc folding and sideways disc displacement and was confirmed in the coronal plane.

Adult↗
PubMed1995

GABAergic inhibitory inputs to subfornical organ neurons in rat slice preparations.

To investigate GABAergic inhibitory inputs to neurons of the subfornical organ (SFO), intracellular recordings were made in rat brain slice preparations. Inhibitory postsynaptic potentials, which occurred spontaneously or were evoked by focal electric stimulation, had reversal potentials of approximately -60 mV, and were almost totally abolished by the GABAA antagonists bicuculline at 3-100 microM or picrotoxin at 50 microM. Following the application of bicuculline or picrotoxin, the resting membrane potentials were decreased by 4-8 mV. GABA at 10-100 microM and the GABAA agonist muscimol at 1-100 microM decreased the membrane resistance and the firing rate in all neurons tested. The reversal potential of the response to muscimol was similar to that for inhibitory postsynaptic potentials. The actions of muscimol persisted in the presence of 1 microM tetrodotoxin, implying that muscimol must act directly on the recorded neurons. These results suggest that there is a tonic inhibitory GABAergic input to SFO neurons which are mainly mediated through GABAA receptors.

Animals↗
PubMed1995

InsP3-gated ion channels in rat olfactory cilia membrane.

InsP3-gated channels present in isolated rat olfactory cilia membranes were studied by recording current fluctuations in cilia membranes fused onto phospholipid bilayers formed at the tip of a patch pipette. We found that InsP3 (1.25 to 30 microM) induced current fluctuations in 18 of 157 otherwise silent bilayers. The ion channels opened by InsP3 could be classified into two groups that differed in terms of conductance and kinetics. One channel, which had a conductance of 37 pS, displayed two current levels, and the larger single channel currents were associated with longer open dwell times. The other channel displayed a larger conductance (103-184 pS). Both types of channels displayed reversal potentials near zero millivolts, consistent with nonspecific cation channels. These experiments suggest that mammalian olfactory neurons possess two types of ciliary InsP3-gated channels.

Animals↗
PubMed1995

Masseter muscle activity and the hypoglossal nerve.

The effects of severing the hypoglossal nerve and resultant restriction of tongue movement was evaluated for its effect on masticatory jaw movement. Electromyograms of masseter muscles in the feeding rat were recorded 3 days after the nerve severing operation at 1 week intervals. Even after severing the lateral branches of the hypoglossal nerve bilaterally, active-feeding behaviour continued with mastication slightly impaired. After severing the medical branches bilaterally, feeding behaviour was rarely observed, but where eating did take place mastication was impaired with marked disturbance in the masticatory rhythm.

Animals↗
PubMed1994

Heparin-binding domain of bovid herpesvirus 1 glycoprotein gIII.

Bovid herpesvirus 1 (BHV-1) glycoprotein gIII functions as a major virus attachment protein through binding to a heparin-like moiety on the host cells. To identify the functional domain, a panel of gIII deletion mutants was constructed, expressed in COS-7 cells, and examined for heparin-binding activity. Mutants with deletion of amino acid residues 103-173 and 324-443 bound to heparin as well as full-length gIII, whereas a mutant with residues 172-337 deleted showed no binding to heparin. In another mutant, with residues 172-211 deleted, the activity was reduced by one-third. These data suggest that the amino acid sequence between residues 172 and 323 contains the functional domain of BHV-1 gIII for heparin-binding and that especially the sequence between residues 212-323 includes a critical site for the activity.

Amino Acid Sequence↗