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Biomedical subjects

E H Huizing

Publications and source records attributed to E H Huizing.

At least 55 records · Page 3Linked to original sources
PubMed1992

Influence of sympathetic and parasympathetic substances in clinical concentrations on human nasal ciliary beat.

The effect of autonomic substances on ciliary beat in mucosal biopsies from the normal human nose was studied. The influence on ciliary beat frequency (CBF) as well as on ciliary beat harmony was measured photoelectrically. The sympathetic beta-agonist isoprenaline was found to have a stimulatory effect on CBF, whereas the alpha-agonist xylometazoline reduced CBF and ciliary beat harmony. The parasympathetic agonist carbachol had a positive effect on CBF. The sympathetic antagonists timolol (beta) and phentolamine (alpha) as well as the parasympathetic antagonist atropine had no effect. The effect of the agonists on ciliary beat was absent when they were administered in combination with their specific antagonists. These experiments indicate that, in clinical concentrations, the autonomic substances modify ciliary beat by a direct effect on the ciliated cells.

Adrenergic alpha-Agonists↗
PubMed1991

Scanning electron microscopy of hair cells, stereocilia and cross-linkage systems in experimentally induced endolymphatic hydrops.

In this study scanning electron microscopy was used to examine the stereocilia and their cross-linkages in guinea pig cochleas 1, 2, 4 and 8 months after endolymphatic sac obliteration. Initial changes were restricted to the stereocilia of the outer hair cells and consisted of a disarrangement and bulging of the stereocilia with an interruption of their cross-linkage systems. Subsequently, the stereocilia became fused and atrophied. Cross-linkages of inner hair cells remained intact. Loss of both outer and inner hair cells started in the apex and progressed towards the base of the cochlea. These findings indicate that early changes in the micro-architecture of the stereocilia may have a mechanical origin, with pressure fluctuations in the scala media possibly playing an important role.

Animals↗
PubMed1991

Cytokeratin expression in the epithelia of the adult human cochlea.

Epithelia can be characterized by the specific expression pattern of their cytokeratin components. Therefore, we investigated the immunohistochemical expression of different cytokeratin subunits in frozen sections of chemically fixed, non-decalcified, adult human cochleas. The organ of Corti and the marginal cells of the stria vascularis showed reactivity for cytokeratin subunits 8, 18 and 19, whereas the other cochlear epithelia in addition expressed cytokeratin 7. The expression of cytokeratins 7, 8, 18 and 19 by the epithelia of the adult human cochlea is typical of "simple" epithelia. The deviant cytokeratin pattern of the organ of Corti and marginal cells of the stria vascularis may well reflect their differences in functional state and/or differentiation as compared to the other cochlear epithelia.

Adult↗
PubMed1991

Differential immunohistochemical detection of cytokeratins and vimentin in the surgically removed human endolymphatic duct and sac.

Immunohistochemical detection of intermediate filament proteins and different subgroups of cytokeratins (Cks) was used to characterize the epithelium of the surgically removed adult human endolymphatic duct (ED) and sac (ES). The epithelium of the ED and ES demonstrated immunostaining for Cks 7, 8, 14, 17, 18 and 19, a pattern typical of so-called "complex" or "mixed" epithelia. This is a remarkable finding, since this pattern differs strikingly from previously reported data on the adult human cochlea and vestibular labyrinth that demonstrated a Ck pattern typical of "simple" (or single-layered) epithelia. Furthermore, the epithelium of the ED and ES demonstrated co-expression of Cks and vimentin. The present data indicate that the epithelium of the ED and ES exhibits another type of epithelial differentiation and demonstrates a higher degree of complexity than the other epithelia in the adult human inner ear.

Adult↗
PubMed1991

Expression of intermediate filament proteins in the adult human cochlea.

The immunohistochemical localization of intermediate filament proteins was studied in frozen sections of chemically fixed, nondecalcified adult human cochleas. Cytokeratins were found in all epithelial cells lining the cochlear duct (including most supporting cells of the organ of Corti) but were absent in the hair cells. Neurofilament proteins were present in the nerve endings at the hair cells, in the neural bundles, and in the ganglion cells. Vimentin staining occurred in most of the supporting structures and was roughly complementary to the regions showing cytokeratin staining and neurofilament staining. However, the region of the spiral prominence and outer sulcus, as well as the pillar cells and Deiters' cells in the organ of Corti, showed coexpression of vimentin and cytokeratins. No definite immunostaining was observed with antibodies to desmin and glial fibrillary acidic protein.

Adult↗
PubMed1991

Early ultrastructural effects of gentamicin cochleotoxicity.

Ultrastructural changes in the cochlear hair cells during the early stages of gentamicin intoxication were investigated after tri-aldehyde primary fixation and OSO4/K4Ru(CN)6 post-fixation. In cochleas treated for 5, 10 and 15 days with gentamicin the individual outer hair cells (OHC1, OHC2 and OHC3) were randomly affected. Degeneration of the inner hair cells was not observed at this stage. Ultrastructurally, the earliest identifiable changes were an increase in lysosomes, proliferation of the endoplasmic reticulum, and formation of Hensen's bodies. This was followed by dilatation of the endoplasmic reticulum and the nuclear envelope, giving rise to extensive cytoplasmic vacuolation. These results demonstrate that gentamicin-induced changes primarily involve the cell's synthetic apparatus. All further intracellular changes occur at a much later stage and are therefore to be considered secondary events.

Animals↗
PubMed1991

Factors influencing ciliary beat measurements.

Investigation of the effect of environmental and pharmacological factors on human respiratory epithelium requires standardization of measuring conditions. Ciliary beat frequency (CBF) and its shift were determined photo-electrically in 43 biopsies of human nasal mucosa. Curette biopsies were compared to forceps biopsies. CBF variation between three different cells of one biopsy sample did not differ for the two biopsy techniques. The ciliated cells of forceps biopsy specimens showed a more constant beating pattern which resulted in a small CBF shift. It appeared that in studying ciliary activity a continuous layer of ciliated cells which is in contact with the basal membrane is required. Therefore forceps biopsies are preferable to curette biopsies. The environmental temperature has to remain constant since CBF is temperature dependent. The pH and osmolarity of the medium do not influence CBF when kept within a certain range. No effect of medium superfusion flow rate was seen.

Basement Membrane↗
PubMed1990

Ciliary beat frequency and the nasal cycle.

The purpose of this study was to investigate whether the ciliary beat frequency (CBF) is influenced by the nasal cycle. Nasal patency was assessed by active anterior rhinomanometry and immediately followed by measuring CBF in biopsies taken from the posterior aspect of the inferior turbinates in 22 healthy volunteers. CBF was determined in three different cells of each biopsy by means of a phase-contrast microscope and a photo-electrical cell. The signal was analyzed by a computed power spectrum. The frequency and its shift were calculated for a period of 20 s. The results were as follows: (1) no correlation was found between CBF and nasal patency; (2) CBF in one cell is constant to a high degree; (3) CBF of different cells in the same biopsy specimen varies considerably.

Adolescent↗
PubMed1990

Progress in temporal bone histopathology. III. An improved technique for immunohistochemical investigation of the adult human inner ear.

A new tissue processing technique is described for immunohistochemistry of the adult human inner ear, avoiding the immuno-compromising step of prolonged decalcification. The procedure allows reliable investigation of frozen sections of the chemically fixed, non-decalcified, adult human cochlea and vestibular sense organs. Preservation of immunoreactivity, combined with satisfying tissue morphology, is illustrated by the immunohistochemical localization of the intermediate filament constituents cytokeratins and neurofilament proteins throughout the membranous labyrinth.

Adult↗
PubMed1990

Cross-sectional anatomy of the nose and paranasal sinuses. A correlative study of computer tomographic images and cryosections.

Anatomic relations in the nose and paranasal sinuses have been studied in computer tomographic images and cryosections, to highlight some details that are of importance for the functional nasal surgeon. From three heads the sections were obtained in three perpendicular planes (coronal, axial and sagittal). The most interesting sections are depicted in three ways: a computer tomographic image, the surface photography of the tissue block in the microtome and a stained section of 20 microns. A number of interesting relations that are clearly depicted are discussed.

Humans↗