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Biomedical subjects

E Grundmann

Publications and source records attributed to E Grundmann.

At least 55 records · Page 3Linked to original sources
PubMed1987

[Space-occupying tumor lesions of the spinal column. An analysis of the material submitted to the Westphalia Bone Tumor Register].

308 tumorous lesions of the spine were identified in the material submitted to the bone tumour register in Münster for recording. Histological examination revealed metastases in 38.6% primary bone tumours in 33.1%, tumour-like lesions in 10% and haematological and lymphatic disease in 13%. 5.3% of all lesions could not be classified in these groups. Primary bone tumours were seen more often than metastases in the cervical spine, the sacrum and the coccyx. 54.9% of the primary bone tumours of the spine were benign. However, only 31.2% of all the tumorous lesions of the spine, metastases included, were benign. The portion of malignant disorders increased with increasing age. The risk of having a malignant spine tumour was 5.9% in patients under 10 years of age but 92.3% in patients in the seventh decade.

Age Factors↗
PubMed1987

[Primary bone tumors and tumor-like lesions of the spine. The accuracy of radiological assessment criteria].

In 79 primary bone tumors and tumorlike lesions of the spine radiological criteria are analyzed, which allow to assess the biological nature. Criteria associated with benign lesions, are: regular, trabecular matrix, osteoplastic matrix, exostotic growth, location in the posterior elements, absent soft tissue mass, and bulging of the vertebra. Criteria suggestive of malignancy are: Soft tissue mass, location inside the vertebral body, no alteration of the vertebral shape, compression fracture, and osteolysis. Benign lesions can be diagnosed with a higher degree of reliability than malignant tumors. Diagnostic accuracy increases in lesions exhibiting more than one criterion of the same group.

Chondroma↗
PubMed1987

Biologic characterization of human bone tumors. VI. The aneurysmal bone cyst: an enzyme histochemical, electron microscopical, and immunohistological study.

The etiology of aneurysmal bone cyst is still unknown. Most theories of the histogenesis of this lesion assume a vascular origin and speculation has focused on the characteristic pseudoendothelial lining of the cyst walls. In the present study, this structure has been subjected to enzyme histochemical, electron microscopical, and immunohistochemical investigation. Of the enzymes tested only alkaline phosphatase was present in the cyst lining. Electron microscopy revealed fibroblast-like cells covering the walls of cystic cavities, but no genuine endothelium, basement membranes or pericytes were identified. For the immunohistochemical studies a panel of poly- and monoclonal antibodies against HLA-DR antigens, mature and immature macrophages/histiocytes, smooth muscle fibers and endothelial cells, as well as the lectin Ulex europaeus I agglutinin were used. None of these markers demonstrated the presupposed vascular characteristics in the cells constituting the pseudoendothelial lining of the cyst walls. Despite current theories to the contrary, it was concluded that aneurysmal bone cyst is unlikely to originate from the vascular system, and that a new concept of its pathogenesis must be sought.

Acid Phosphatase↗
PubMed1987

[Pathology of spinal tumors].

The Bone Tumor Registry of Westphalia contains data on 7,400 tumors and tumor-like lesions of bone, 135 primary spinal tumors, 187 metastases, 98 plasmacytomas, 4 extranodal manifestations of Hodgkin and non-Hodgkin lymphomas of the vertebral column. The most frequent type of primary tumor is the chordoma (35 cases), followed by osteoblastoma (16 cases), eosinophil granuloma (16), and hemangioma (12 cases). Most of the metastases derive from carcinoma of the breast, bronchial carcinoma, or prostate carcinoma. The present review concentrates on differential diagnosis by means of histological examination, with particular reference to immunohistological methods. In addition, the necessity for complementary assessment of the X-ray findings and histology is emphasized. In particular, the current status of knowledge on the prognosis of primary spinal tumors is presented. In our experience, the preparation of nondecalcified plastic sections has proved especially valuable for diagnostic procedures using punch biopsy specimens.

Humans↗
PubMed1986

DNA stemline heterogeneity in colorectal cancer.

The current study was carried out on 88 colorectal carcinomas to assess the degree of intratumor heterogeneity as reflected by multiple aneuploid DNA stemlines and their relation to tumor stage and morphologic differentiation. Each tumor was segregated into an average of nine specimens (3-15), which were analyzed separately. DNA aneuploidies were identified in 72 cases (82%), 29 revealing multiple aneuploid DNA stemlines with up to four aneuploid subpopulations. In 10 of the 29 carcinomas with DNA stemline heterogeneity, a ratio of 2:1 was calculated from the different DNA indices, possibly indicating that the additional DNA stemline emerged from the first one by doubling its DNA content. No correlation was found between the overall frequency of DNA aneuploidies or heterogeneous DNA stemlines and the tumor stage according to Dukes' staging. Well-differentiated carcinomas tended to express aneuploid DNA stemlines more frequently than moderately or poorly differentiated tumors, although the morphologic intratumor heterogeneity did not correspond to the appearance of multiple aneuploid DNA stemlines. These data indicate a high degree of intraneoplastic diversity in colorectal cancer and emphasize the usefulness of DNA analyses for the quantitative assessment of tumor heterogeneity.

Aneuploidy↗
PubMed1986

Haematogenous metastatic patterns in colonic carcinoma: an analysis of 1541 necropsies.

The sequence of events in haematogenous metastasis from colonic carcinoma was analysed, using 1541 necropsy reports from 16 centres. The findings are consistent with the cascade hypothesis that metastases develop in discrete steps, first in the liver, next in the lungs and finally, in other sites. Deviations of necropsy findings from the cascade model are largely explained on the basis of false negative reports. In only 216 of 1194 cases was there suggestive evidence that metastatic patterns (excluding lymph nodes) were causally related to lymphatic or non-haematogenous pathways. The incidence of metastatic involvement in 'other' (quaternary) sites correlated with target organ blood-flow (ml min-) per g, only when bone marrow and thyroid were excluded. In the thyroid the incidence was lower than expected on the basis of blood flow per g tissue; this may indicate that the thyroid is an unfavourable site for metastatic growth of colonic carcinoma. In the bone marrow it is higher; the latter may be due to delivery of cancer cells via both arterial blood and the vertebral venous plexus. Recognition of this pattern of metastases in the bone marrow could be important with respect of diagnosis and therapy, in patients with colonic carcinoma.

Aged↗
PubMed1986

Improved grading of bone tumors with the monoclonal antibody Ki-67.

A total of 60 bone tumors and tumor-like lesions presenting various grades of malignancy were investigated immunohistologically with the monoclonal antibody Ki-67 directed against a cell proliferation-associated nuclear antigen. The results obtained agree well with those of flow cytometric and autoradiographic studies on similar tumor entities. The monoclonal antibody Ki-67 was found to be a handy and reliable tool for improved grading of bone tumors.

Antibodies, Monoclonal↗
PubMed1986

Macrophages and T lymphocytes infiltrating the rat mammary carcinoma HH9-cl 14 in progressive and regressive tumor growth. An immunohistological study.

Ascites tumor cells (2 X 10(6] of a DMBA-induced rat mammary adenocarcinoma (HH9-cl 14) were injected s.c. into tumor-free syngeneic female rats and produced a continuously growing solid tumor in all animals of this group. Inoculation of 2 X 10(7) cells induced a first brief period of tumor growth, followed by complete tumor regression from the 2nd until the 5th week after injection. Both the progressive and the regressive tumors were analyzed immunohistologically at different stages with monoclonal antibodies against different T lymphocytes and macrophages. Obviously these cells appear in different quantity and quality, during the hosts immune response. Possible interactions of T lymphocytes and macrophages with tumor cells are discussed.

Animals↗
PubMed1986

Biological characterization of human bone tumors. VIII. Expression of HLA-DR antigens in bone tumors and tumor-like lesions.

A total of 45 cases of bone tumors and tumor-like lesions were studied in order to determine the expression of an HLA-DR antigen by the monoclonal antibody 910-D-7, and its possible correlation with histology, using the indirect immunoperoxidase method on frozen sections. The pattern of antigen expression was nearly constant for the individual cell types, though varying in intensity, and did not depend on the biological behavior of the respective lesions. No clear correlation could be established between antigen expression and cell maturation. Although the biological significance of antigen expression in these tumors is not yet understood, it is clear that here, too, the mere presence of an HLA-DR antigen cannot be interpreted as a sign of malignant transformation.

Bone Cysts↗
PubMed1986

The ultrastructural heterogeneity of potentially preneoplastic lesions in the human bronchial mucosa.

170 consecutive biopsies from the bronchial tree were subjected to ultrastructural analysis. 62 were identified as preneoplastic lesions: of these, 7 as goblet cell hyperplasia, 20 as basal cell hyperplasia, 17 as combined goblet and basal cell hyperplasia, 10 as epidermoid metaplasia, and 8 as dysplasia. The ultrastructural features of these lesions are described. According to these results, preneoplastic lesions of the human bronchial mucosa are composed of heterogeneous populations that display mixed or combined morphologic features signaling abnormal differentiation patterns at this stage. The findings are tentatively correlated with bronchial carcinoma cell heterogeneity and histogenesis.

Adult↗
PubMed1986

[X-ray morphology and pathologic anatomy of osteoblastoma].

Between 1974 and 1984, 24 patients with osteoblastomas were entered in the bone tumour registry of the Gerhard Domagk Institute of Pathology and Institute for Clinical Radiology of Westphalia. These cases are evaluated. Osteoblastoma is a benign primary bone tumour with a peak incidence between ten and 20 years and a sex ratio of 18 males to 4 females in our series. The site of predilection is the spine (59%) with long bones in second place (32%). The tumor is mostly situated in the medulla. Radiologically it usually appears as a well demarcated translucency with a narrow rim and marginal sclerosis. Variations in radiological appearance and the histology and treatment are described. A malignant variant, the "aggressive osteoblastoma", is discussed and one case is described. The classification of these cases is considered.

Adolescent↗