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Biomedical subjects

E Gazit

Publications and source records attributed to E Gazit.

At least 109 records · Page 6Linked to original sources
PubMed1989

Non-symmetric subcutaneous lipomatosis associated with familial combined hyperlipidaemia.

A family with familial combined hyperlipidaemia in which affected members had nonsymmetric subcutaneous lipomatosis (NSSCL) is described. Affected members had high serum levels of total cholesterol, low density lipoprotein (LDL) cholesterol and high density lipoprotein (HDL) cholesterol. By contrast, family members without NSSCL had normal lipid levels. There was also a correlation between the degree of hyperlipidaemia and the amount of subcutaneous lipomas. The occurrence of hyperlipidaemia in family members with NSSCL suggests the existence of a genetic linkage between these two characteristics, but did not show any association with HLA haplotyping. To our knowledge this association between lipid abnormalities and NSSCL has not been previously reported.

Adolescent↗
PubMed1989

IL-1 production by T6 (CD1a) positive cord blood mononuclear cells (Langerhan's cell precursors?).

Human cord blood (CB) mononuclear cells were fractionated into peanut agglutinin positive (PNA+) and PNA negative (PNA-) subsets. The PNA+ subset was enriched for T6+(CD1a)Ia+ cells, which we have previously shown to resemble the Langerhans cells (LCs) of the skin, and therefore described as circulating LCs precursors. Supernatants of PNA+ and PNA- cells, and of FACS purified populations of T6+ CB cells, cultured with and without LPS, were tested for IL-1 activity. It was found that cord blood PNA+ mononuclear cells as well as purified populations of T6+ CB cells produce significant amounts of, both extracellular and cell associated, IL-1 as compared to PNA- and T6- cells, and comparable to those produced by macrophages. LPS stimulation mainly affected T6+ cells. It can be concluded that cord blood T6+ cells, presumably LCs precursors, are capable of IL-1 production.

Antigens, CD1↗
PubMed1988

T6 positive cells in the peripheral blood of burn patients: are they Langerhans cells precursors?

Peripheral blood mononuclear cells of 14 patients suffering thermal injury were separated by affinity chromatography on peanut agglutinin (PNA) coupled to Sepharose macrobeads. The resulting PNA positive subset was 14% of the total mononuclear population. About 30% of these cells were found to coexpress T6(CD1), Ia-like and the myeloid differentiation antigens My4(CDw14) and Mo1(CD11). In comparison, the PNA+ subset from normal blood donors (about 5% of total mononuclear cells) contained mature monocytes that were found to be T6 negative. Electron microscopic studies using immunogold labeling showed that the T6 positive cells were slightly smaller than monocytes but larger than the classical lymphocytes and had common morphologic features with the Langerhans cells of the skin. Considering that patients suffering extensive damage of the epidermis require fast renewal of all skin elements, it is possible that the cells we identified in their peripheral blood are the precursors of the Langerhans cells of the skin en route from bone marrow to the epidermis.

Adolescent↗
PubMed1988

Association of HLA-B35 with mucocutaneous lesions in Israeli patients with rheumatoid arthritis receiving gold treatment.

Seventy-four Israeli patients with rheumatoid arthritis (RA) were studied for possible association between HLA antigens and adverse reactions to gold treatment (aurothioglucose). HLA-B35 was significantly increased in patients who developed gold induced mucocutaneous lesions. These results are in accord with earlier reports, despite the different genetic background of the Israeli RA population and the different type of gold compound used.

Arthritis, Rheumatoid↗
PubMed1988

Methotrexate in rheumatoid arthritis: a prospective study in Israeli patients with immunogenetic correlations.

In a prospective open study 44 Israeli patients with rheumatoid arthritis were treated with weekly low dose methotrexate (MTX) for up to 36 months. Nine patients withdrew from the study: six because of side effects and three due to inefficacy. One patient died of septicaemia following septic arthritis. Significant improvement, graded by Ritchie articular index, grip strength, physician's global assessment, erythrocyte sedimentation rate (ESR), and platelet counts, was noticed in response to treatment. Seronegative patients had a better clinical response. Transient gastrointestinal symptoms were common and correlated with increases of serum aspartate transaminase (AST). HLA-DR1 and DR7 were significantly associated with increased serum AST concentrations.

Aged↗
PubMed1988

HLA-A11 is associated with poor prognosis in childhood acute lymphoblastic leukemia (ALL).

A possible association between HLA antigens, susceptibility or resistance to leukemia, and responsiveness to treatment has been studied in 144 patients with childhood acute lymphoblastic leukemia (ALL) and compared to other prognostic factors, i.e. white blood cell (WBC) counts, age at onset, sex, ethnic origin, and cell surface markers. All sequentially newly diagnosed children (97) comprised the group for the prospective study (PSG) and were followed for 6 years. The group included 37 patients classified as T-ALL, 41 as CALLA+, 27 as NULL, 12 as B and pre-B, and 27 unclassified patients, who were diagnosed before 1980. During the follow-up period, 45 patients of the PSG died. Forty-seven patients designated long-term survivors (LTS) have been followed 6-20 years after diagnosis, having completed a 3-5 year course of anti-leukemia therapy, and having remained disease free thereafter. High WBC counts at diagnosis and T-cell-surface markers were associated with poor prognosis, as were enthnic origin and specific HLA antigens. Thus, there was one (1) a significant increase in HLA-A30 and a decrease in HLA B-14 in the PSG Jewish patients; and (2) a complete absence of HLA-ALL in LTS while, in the PSG, 8 of 9 HLA-All-positive patients died during the follow-up period. This suggests that HLA-All is associated with poor prognosis in childhood ALL.

Adolescent↗
PubMed1987

Lymphocytes modulated by beta interferon express new non-HLA-A,B,C class I antigens.

Peripheral blood lymphocytes were cultured with recombinant beta interferon (INF-lymph). Three days in culture and 5 X 10(4) units/ml produced the best modulatory effect. Platelet absorbed alloantisera previously shown to contain non-HLA-A,B,C class I antibodies were tested with the INF-lymph. The serologic reactivity could be blocked by turkey sera to human B2-microglobulin but not by turkey anti-Ia-like. Absorption studies with INF-lymph obtained from donors expressing different HLA antigens indicated that the serologic reactivity is not due to HLA. Fourteen sera appeared to cluster in three groups, only one of which was found to be associated with HLA (HLA-A1). Antigenic modulation of INF-lymph obtained from HLA-A1+ individuals with HLA-A1 typing alloantisera eliminated subsequent lysis mediated by HLA-A1 alloantibodies and complement but not lysis with platelet absorbed antibodies included in the cluster associated with HLA-A1. These findings suggest independence from HLA-A1 and coexpression of HLA-A1 and A1-associated class I antigens. Family studies indicated that the reactivities segregated with HLA thus mapping it to chromosome VI. Similar to what we have described with PHA, beta interferon modulates lymphocytes and induces the expression of new class I differentiation antigens probably analogous to the murine Qa-T1a antigens.

Blood Platelets↗
PubMed1987

Internalization by receptor-mediated endocytosis of T6 (CD1 "NA1/34") surface antigen in T6 positive human cord blood cells (Langerhans cell precursors?).

A subset of T6 positive cells was recently separated from normal human cord blood mononuclear cells. It was shown to coexpress HLA-DR and myeloid differentiation antigens (Mo1, MY4). The phenotype and ultrastructure of the cells suggested that these T6 positive cells might be the precursors of the Langerhans cells of the skin. We have previously demonstrated by immunogold labeling techniques that the T6 surface antigen of human Langerhans cells of the skin is internalized in unfixed Langerhans cells or indeterminate cells by a process of receptor-mediated endocytosis. This process involved the formation of coated pits, coated vesicles, endosomes and lysosomes. Following this process, in Langerhans cells, gold labeled Birbeck granules appeared in the cell center often in continuity with endosomes. In the present study, we used an indirect immunogold labeling technique to reveal the T6 antigen present on the surface of living T6 positive cord blood mononuclear cells. We observed the internalization of the T6 surface antigen by a process of receptor-mediated endocytosis similar to that described in Langerhans cells of the skin. This process, however, was not followed by the appearance of intracytoplasmic Birbeck granules.

Antigens, Differentiation, T-Lymphocyte↗
PubMed1986

Reproducibility of occlusal marking techniques.

Our study was designed to (1) test clinically the reproducibility of the NPT by comparing two consecutive occlusal records and records made at 1-month intervals (2) test the reproducibility of a color-marking technique under the same conditions, and (3) compare the location of occlusal contacts as registered by these two techniques. Results showed that neither technique is highly reproducible. However, the NPT is more reproducible than the CMT. The advantages and disadvantages of the NPT and the CMT were described. It was suggested that more research should be conducted to improve the accuracy of occlusal record techniques.

Dental Articulators↗
PubMed1986

A subset of human cord blood mononuclear cells is similar to Langerhans cells of the skin: a study with peanut agglutinin and monoclonal antibodies.

Mononuclear cells were fractionated from human cord blood by affinity chromatography on immobilized peanut agglutinin, as previously described (Rosenberg et al., Hum Immunol 7:67, 1983). The PNA+ subset was found to be composed mainly of a population of cells phenotyped as Ia+, T6+, M01+, and MY4+. The presence of mononuclear cells coexpressing these antigens was demonstrated by three techniques: double labeling immunofluorescence using FITC and rhodamine conjugated goat antimouse IgG; fluorescence activated cell sorter (FACS); and by direct counting (under the microscope) of cells stained by either individual or a combination of a variety of monoclonal antibodies. The PNA+ cells expressed cytoplasmic structures similar to Birbeck granules. In view of the fact that Langerhans cells of the skin share a similar phenotype and express Birbeck granules, we suggest that this subset may be the precursor of the Langerhans cells of the skin. In addition, these cells may also be the precursors of the dendritic cells found in the spleen, lymph nodes, thymus, and liver.

Antibodies, Monoclonal↗
PubMed1986

Fractionation of human bone-marrow mononuclear cells with peanut agglutinin: phenotypic characterization with monoclonal antibodies.

Bone marrow mononuclear cells were fractionated by affinity chromatography on immobilized peanut agglutinin (PNA). The resulting PNA+ fraction represented 10% of the total cell number. Twenty percent of the cells within the PNA+ compartment coexpressed the T6, Ia, Mo1, and My4 differentiation antigens and possessed Fc and C3 receptors. The similarity in cell surface antigen phenotype led us to hypothesize that this subset may be a cellular precursor of dendritic cells found in the skin (Langerhans cells) or in the parenchimal organs of the body (D-cells).

Antibodies, Monoclonal↗
PubMed1986

Alteration of antigenic expression of human myeloid precursor cells (CFU-GM) in long-term cultures.

Comparison of myelopoiesis in continuous human bone marrow and cord blood cultures was performed. The presence of major histocompatibility complex (MHC) antigens on myeloid precursors (CFU-GM) from cord blood was confirmed using monoclonal antibodies (MoAb) defining class I (i.e., anti-beta 2-microglobulin (B2, MG1) anti-HLA-A,B heavy chain (W6/32), and anti-HLA locus B(4E), and class II (HLA-DR (7,2] antigens. The effect of long-term culture on the expression of these antigens was investigated. Our studies indicate that following prolonged in vitro culture there is a decrease in the expression of MHC antigens on CFU-GM. Our observations provide a rationale for considering the use of cord blood leukocytes subjected to long-term culture as an alternative to allogeneic hematopoietic cell transplantation.

Aging↗
PubMed1986

Differentiation of bone marrow cells from myelodysplastic patients in the presence of 1,25 dihydroxyvitamin D3 or 13-cis retinoic acid.

The separate effects of vitamin D3 (1,25(OH)2D3) and 13-cis retinoic acid on the differentiation in liquid culture of marrow cells from seven patients with myelodysplastic syndrome (MDS) were studied. Following incubation with 1,25(OH)2D3, an increasing number of myeloid cells acquired the morphological appearance of mature monocyte-macrophages and reacted positively to fluoride-sensitive naphthyl acetate esterase and specifically bound My4 monoclonal antibody (McAb). Incubation of bone marrow cells with 13-cis retinoic acid enhanced the number of cells with the morphological appearance of metamyelocytes and mature granulocytes as well as those that reacted positively with AS-D naphthol chloroacetate esterase. The results suggest that the differentiation pattern of myeloid precursor cells from MDS patients can be modulated by 1,25(OH)2D3 and 13-cis retinoic acid.

Aged↗