Biomedical subjects

E Espinosa

Publications and source records attributed to E Espinosa.

130 records · Page 8Linked to original sources

PubMedDate not supplied

Effect of the extender supplement Equex-STM on cryopreserved semen in the Assaf sheep.

This study was designed to evaluate the effect of adding the detergent Equex-STM to the extender used to dilute semen for cryopreservation on several indicators of sperm preservation. Two consecutive ejaculates per day were obtained from 5 Assaf sheep on two days out of every week over three alternate months. The freezing protocol involved diluting the semen in Fiser's extender, to which 0.7 percent Equex-STM was added or omitted before cryopreserving the semen in straws by exposure to nitrogen vapor. Equex-STM supplementation gave rise to significantly (p=0.05) improved sperm quality variables after different periods of freezing (0 hours, 1 week and 1 month). The variables examined were: individual motility, viability, acrosome integrity, plasma membrane integrity (HOS test) and morphological anomalies. This improvement was independent of the ram and month of testing. In a second experiment in which we incubated the semen (0 and 6 hours) at 37 degree C after thawing, Equex-STM also showed a beneficial effect on sperm quality.

Animals↗

PubMedDate not supplied

Absence of liver antigens in transplantable hepatomas. A possible relationship to the tumor aggressiveness.

Presence of liver antigens in transplantable Morris hepatomas of varying aggressiveness was investigated by immunodiffusion and absorption methods. The most malignant and undifferentiated of these, hepatoma 7777, was found defective in two liver antigens, whereas the less malignant hepatomas, 5123tc and 9618A, did have them. Both antigens had distinct occurrence in tissues and hepatic subcellular fractions and behaved like proteins of electrophoretic mobilities of serum gamma globulins with molecular weights of 51,000 and 240,000, respectively. Since hepatoma 7777 is the least differentiated, fastest growing, and more metastatic of the tumors studied, these properties would appear to be associated with the absence of the antigens.

Animals↗

PubMedDate not supplied

The occurrence and properties of a serum hepatic antigen in various liver diseases.

A liver antigen, LA-1, was detected by immunoelectrophoresis in the sera of 79 percent of patients with viral hepatitis A, 77 percent of patients with viral hepatitis B, and 62 percent of patients with alcoholic hepatitis. The occurrence of LA-1 was lower in liver cirrhosis (28 percent) and primary extra-hepatic disorders (18 percent) and absent in the sera of healthy subjects. Liver antigen, LA-1, is detectable in human kidney, lung, spleen, heart and skeletal muscle, and rhesus monkey liver; however, it is not found in the livers from dog, rat, cat, or pig. This antigen is characterized as a protein with beta-globulin electrophoretic mobility and a molecular weight in the range 67,000 to 80,000, which distinguishes it from liver ferritin and previously defined bile antigens and liver-specific antigens detected in sera from patients with liver diseases. The presence of LA-1 in serum appears to denote non-specific liver injury.

Animals↗

PubMedDate not supplied

Phase I study of UFT plus leucovorin in advanced colorectal cancer: a double modulation proposal.

Twenty-six patients with advanced colorectal cancer were treated with UFT and leucovorin (LV). On day 1, patients received LV 500 mg/m2 in IV infusion, followed by 15 mg/12 h for 13 days. On days 1 to 14, patients took oral UFT twice daily. Three cycles were given every 28 days, unless grade III-IV toxicity appeared. The initial dose of UFT (200 mg/day) was increased until 800 mg/day. Dose limiting toxicities were stomatitis, diarrhea and epigastralgia. The maximum tolerated dose of UFT was 390 +/- 10 mg/m2. Three out of 24 evaluable patients achieved a partial response and 1 a complete response with UFT doses of 260 to 390 mg/m2. These results warrant confirmation in phase II studies.

Administration, Oral↗