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Biomedical subjects

E Dupont

Publications and source records attributed to E Dupont.

At least 271 records · Page 15Linked to original sources
PubMed1971

The measurement of glomerular filtration pressure from sieving data for macromolecules.

It is possible to derive the GFP from sieving data by two means and with the following qualifications: 1. Assuming the membrane isoporous, it is possible to calculate GFP by applying Poiseuille's law. The analysis of the sieving curve should then be limited to molecules with a radius between 21 and 41 A. 2. Assuming a log-normal distribution of pore radii, it is possible to obtain a value for GFP by computation. In this case the values for phi higher than 0.7 will not be considered in the calculation of sigmaE used to determine the most satisfactory adjustment of the calculated sieving curve to the experimental data. Although the second method allows a perfect adjustment of the curves, it gives more scattered results than the first for the calculation of GFP. However, a close correlation exists between both sets of results.

Animals↗
PubMedDate not supplied

Modulation of the contact hypersensitivity response by AE-941 (Neovastat), a novel antiangiogenic agent.

BACKGROUND: AE-941 (Neovastat) is an angiogenesis inhibitor noted to have antiinflammatory properties. OBJECTIVE: We tested Neovastat in a contact hypersensitivity (CHS) model to determine the mechanism of action of its antiinflammatory effects. METHODS: Neovastat was orally administered (200 mg/kg/day) during the sensitization and challenge phases of a murine CHS assay and inflammatory responses were measured. Subsequent assays were performed on mice treated with Neovastat or Cortisone (120 mg/kg/day, IP) and differential mRNA expression of several pro- and antiinflammatory cytokines was quantified using RT-PCR. RESULTS: Neovastat decreased inflammation by 39% when administered during sensitization but did not alter the CHS response when given during the challenge phase. Neovastat significantly induced IL-10 expression in skin and skin-draining lymph nodes (49% and 45%, respectively) and decreased IFNgamma expression in the lymph nodes (35%). CONCLUSION: Antiinflammatory effects of Neovastat observed in CHS could be linked to modulation of cytokines early in the sensitization phase.

Administration, Oral↗
PubMedDate not supplied

Analysis of the peripheral T-cell compartment in the MHC class II deficiency syndrome.

To analyse the impact of lack of MHC class II expression on the composition of the peripheral T-cell compartment in man, the expression characteristics of several membrane antigens were examined on peripheral blood lymphocytes (PBL) and cultured T cells derived from an MHC-class-II-deficient patient. No MHC class II expression could be detected on either PBL or activated T cells. Moreover, the expression of MHC class I was reduced both on PBL and in vitro activated T cells compared to the healthy control. However, the reduced expression of CD26 observed on the PBL of the patient was restored after in vitro expansion. Despite the presumably class-II-deficient thymic environment, a distinct but reduced single CD4+ T-cell population was observed in the PBL of the patient. After in vitro expansion, the percentage of CD4+ cells dropped even further, most likely due to a proliferative disadvantage, compared to the single CD8+ T-cell population. However, proliferation analysis showed that T-cell activation via the TcR/CD3 pathway is not affected by the MHC class II deficiency.

Antigens, CD↗