Partial amino acid sequences of a 49 kDa apyrase (ATP diphosphohydrolase, EC 3.6.1.5) from the cytoskeletal fraction of etiolated pea stems were used to derive oligonucleotide DNA primers to generate a cDNA fragment of pea apyrase mRNA by RT-PCR and these primers were used to screen a pea stem cDNA library. Two almost identical cDNAs differing in just 6 nucleotides within the coding regions were found, and these cDNA sequences were used to clone genomic fragments by PCR. Two nearly identical gene fragments containing 8 exons and 7 introns were obtained. One of them (H-type) encoded the mRNA sequence described by Hsieh et al. (1996) (DDBJ/EMBL/GenBank Z32743), while the other (S-type) differed by the same 6 nucleotides as the mRNAs, suggesting that these genes may be alleles. The six nucleotide differences between these two alleles were found solely in the first exon, and these mutation sites had two types of consensus sequences. These mRNAs were found with varying lengths of 3' untranslated regions (3'-UTR). There are some similarities between the 3'-UTR of these mRNAs and those of actin and actin binding proteins in plants. The putative roles of the 3'-UTR and alternative polyadenylation sites are discussed in relation to their possible role in targeting the mRNAs to different subcellular compartments.

Actins↗

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Assessing the assessments: a comparison of two clinical pragmatic profiles.

Recent years have seen a growth in interest in pragmatic aspects of communication, and the application of this area to language disorders. This has led to the development of a range of "pragmatic profiles" to assess the disordered language of children and adults. In this study we investigate two such profiles designed for use with aphasic patients. In particular we look at aspects of interscorer reliability (comparing scorers from different linguistic traditions), scoring sensitivity (two- versus five-point scales), and diagnostic accuracy (the ability to differentiate different aphasic syndromes). We conclude that training in scoring these profiles must be uniform, but also that greater sensitivity is needed in the categories available.

Aged↗

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Effect of clomiphene citrate therapy on postcoital tests in successive treatment cycles including response to supplemental estrogen therapy.

Debate continues as to whether clomiphene citrate (CC) has an adverse effect on cervical mucus. Some researchers conclude that adverse effects maybe related to hormonal abnormalities, e.g., premature luteinization or improper timing. This study evaluated the initial and accumulative effects of CC on postcoital testing (PCT) and attempted to determine if supplemental estrogen (E) improves PCT and/or inhibits follicular maturation. The majority of patients taking CC had poor PCT tests even on the first treatment cycle. Good initial PCTs tended to remain so in subsequent cycles or at least to respond to subsequent supplemental E. Poor PCTs responding to E tended to continue to respond to the combination. PCTs not responding to E therapy continued this pattern in subsequent cycles. Supplemental E did not suppress follicular maturation.

Animals↗

PubMedDate not supplied

Accountability in nursing. 8. Does money make a difference?

Explore the source record for details and available documents.

Budgets↗