Biomedical subjects

E David

Publications and source records attributed to E David.

166 records · Page 10Linked to original sources

PubMedDate not supplied

[The sensitivity of Salmonella strains in diarrheal disease to new quinolones compared with other antimicrobial substances].

The sensitivity of 59 Salmonella strains isolated in children with acute diarrhoea was tested against the new quinolones like: Ciproflaxicin (CIP), Norfloxacin (NOR) and Ofloxacin (OFX), as compared to the sensitivity against same aminosides: Gentamicin (GM), Amikacin (AN) against cephalosporins: Ceftazidime (CAZ), Cefalotine (CF) and other currently used antimicrobial agents: Tetraciclin (T), Ampicilin (A), Cloramfenicol (C), Furazolidon (FU). The majority of the studied Salmonella strains, 43 out of 59 strains, belonged to the serotype typhimurium, the most frequently serotype isolated in our geographical area. A very high percentage of Salmonella strains were sensitive against the three quinolones: 98,30% sensitive against NOR, 91,5% sensitive against OFX, 91,50% sensitive against CIP and 96,6% sensitive against AN. In contrast, the Salmonella strains sensitivity was lower in the other tested antimicrobial substances: C (32,2% sensitive strains), GM (8,5%), A (16,9%), CF (11,9%), T (3,4%), FU (1,7%). Out of 59 strains, 45 where resistant to more than four antibiotics, the most often observed pattern was: A, CAZ, CF, GM, T, C, FU.

4-Quinolones↗

PubMedDate not supplied

[The virulence of E. coli strains isolated in urinary infections].

E. coli has been considered to be the most common pathogenic agent associated with the urinary tract infections. The pathogenic features of E. coli strains mainly the adherence, hemolysine and cytotoxine production (CNF) correlated with the affiliation to a specific serological type, provided a subject of active research. In the present study 104 strains of E. coli isolated in patients with urinary tract infections (hospitalised children and adults and outpatient children) were tested for their pathogenic features. The pyelonephritogenic capacity was assessed by the manose-resistant haemagglutination (HAMR) tested with human erythrocytes in 42.30% of strains. The haemolysine production was found in a significant number of strains (32.60%; 38.20% of this percentage enhanced the strains virulence by toxin elaboration (CNF)). The haemolysine production was mostly observed in the strains isolated in hospitalised children while the HAMR was more frequent in outpatient children. The increased incidence of the O6 serogroup among the uropathogenic E. coli strains (41.40%) was mainly recorded in hospitalised children; this finding indicates the O6 serogroup circulation within the health care units. The determination of the pathogenic features, chiefly of the pyelonephritogenic ones has been very useful for the disease prognosis and treatment.

Adult↗

PubMedDate not supplied

[Erythropoietin and obstetric factors--evaluation with special reference to sex differences].

OBJECTIVE: We evaluated fetal Erythropoietin (EPO) in a clinical obstetrical environment, aiming to collect more information upon its role in diagnosis of fetal stress. As it is known that EPO cannot pass the placental barrier, we restricted to measure the concentration in the umbilical artery (UA) immediately after delivery. PATIENTS AND METHODS: EPO was correlated to relevant obstetrical parameters. We looked for each parameter that was tested for differences in fetal sex. For this purpose, the statistics were performed by means of the Behrens-Fisher-Test (meaning a special application of the Student-T-Test). This device is an algorithm developed to compare regressions. For significance, a confidence level of less 5% was accepted. For the rest, we only used descriptive devices. RESULTS: We found weak but positive correlation to gestational age for both sexes. No correlation was calculated for the duration of parturition or the birth weight. Highly significant differences were found for respiratory values of blood gases, taken from the UA when differentiated for fetal sex: males showed a strong and negative correlation for pO2 (r(m)= -0.45); the female EPO-level was not influenced by decreasing O2-level (r(w)= +0.06, p > 0.01). Similar results could be found for pCO2. (r(m)= +0.36; r(w)= -0.07; p < 0.01. Not significant, but comparable differences for the EPO-level in fetal blood were found for pH and base deficit. CONCLUSIONS: If we accept that the EPO-level in fetal blood rises with relevant oxygen deficiency, then females seems to be better protected against damage from distress. This observation is congruent with the clinical experience from the authors who have the subjective impression that female sex is advantageous in harmful deliveries.

Birth Weight↗

PubMedDate not supplied

Plasmid profile analysis and antibiotic resistance of Salmonella strains from clinical isolates in Cluj-Napoca.

Resistance patterns, plasmid profiles and the genetic resistance determinants were investigated in 38 isolates of Salmonella enterica serotype Typhimurium and 19 isolates of Salmonella enterica serovar Enteritidis derived from children hospitalized in two clinics in Cluj-Napoca, during the period of 1995-1997. Incidence of plasmid and antibiotic resistance was very high in Salmonella typhimurium isolates. All strains were resistant to almost all antibiotics tested but susceptible to the third generation cephalosporines and fluoroquinolones. We identified three resistance patterns and six plasmid profiles. Each plasmid profile was characterized by the presence of two large plasmids of 150-180 Kbp. Approximately 60% of strains harbored three or four small plasmids of 1.3 to 9.5 Kbp. The plasmids of 8.5 Kbp encoded resistance to beta-lactam antibiotics and were non-conjugative. The other small plasmids were cryptic and also non-conjugative. Salmonella enteritidis isolates were susceptible to many antibiotics, except Tetracycline and Trimethoprim-Sulfamethoxazole. We identified three different resistance patterns but nine plasmid profiles. All plasmid profiles were characterized by the presence of a large plasmid (> 100 Kbp). The number and the diversity of small plasmids were higher than in S. typhimurium strains. There was no parallelism between resistance and plasmid profile: for the same resistance pattern a number of two or three plasmid profiles were found. Our conclusions are that Salmonella typhimurium strains were multiresistant to antibiotics and that many genetically different strains of Salmonella typhimurium and Salmonella enteritidis were responsible for gastroenteritis in children from Cluj County. The increasing antibiotic resistance highlights the need for more refined methods in genetic and epidemiological characterization of bacteria involved in gastrointestinal infections.

Anti-Bacterial Agents↗