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Biomedical subjects

E Dahl

Publications and source records attributed to E Dahl.

At least 163 records · Page 9Linked to original sources
PubMed1983

Influence of X-ray irradiation on the ultrastructure of rat submandibular gland striated-duct cells.

Previous investigations have indicated that striated-duct cells react to stimulation with an apocrine secretion, morphologically demonstrated by bleb-like projections of the apical cytoplasm. Since bleb formation as an ultrastructural feature also has been debated and sometimes interpreted as a fixation artifact, it was considered essential to extend the studies by exposing the submandibular gland to X rays to establish whether such treatment would have any influence on the formation of blebs. The material used in the present study consisted of rat submandibular glands exposed to X rays in the range of 200-1800 rad. The glands were examined by both SEM and TEM. The duct cells exposed to 200 rad appeared normal, with no sign of alteration in their ability to produce blebs, whereas duct cells exposed to 750 rad showed no sign of bleb formation. Some of the duct cells exposed to 1800 rad showed considerable morphological changes, consistent with oncotic transformation. The results support the conclusion that bleb formation is a normal morphological feature and not an artifact. This study also indicates that the functional activity of the cells is reduced after exposure to X rays.

Animals↗
PubMed1983

Morphometric studies of parietal cells during basal conditions and during stimulation with pentagastrin in healthy subjects.

A morphometric study was carried out of parietal cells from seven healthy volunteers. Under gastroscopical control, biopsies were taken with a hydraulic capsule before and 30 min after stimulation with pentagastrin. No significant change was found in the absolute volume of the parietal cells or their nuclei during stimulation. When median values for the ultrastructure were compared, microvilli constituted 4.6% of the parietal cell volume during basal conditions and 9.3% during stimulation with pentagastrin. Tubulovesicular structures constituted 10.9% of the cell volume during basal conditions and 1.9% during stimulation. These ultrastructural differences between basal and stimulated conditions were statistically significant, with P = 0.02 using Wilcoxon's test for paired data. Similarly significant changes were found in the membrane area of the secretory canaliculus and the tubulovesicular structures.

Adolescent↗
PubMed1982

Classification of plastic-embedded rat seminiferous epithelium prior to electron microscopy.

Systematic electron microscopic studies of the changes which take place during the cycle of the rat seminiferous epithelium require that each of the 14 stages of the cycle are identified in the light microscope in plastic-embedded material allowing each stage to be trimmed out separately before the electron microscopic study. The classification of the 14 stages has originally been based on the morphological characteristics seen in paraffin-embedded tissue after PAS-staining. PAS-staining is less effective in plastic-embedded material, and this may be a main reason why electron microscopic findings of the seminiferous epithelium relatively seldom are related to the stages of the cycle. In the present paper a method is described by which the 14 stages of the cycle can be easily identified in the light microscope in plastic-embedded tissue. Some of the stages could be subdivided and a total of 23 stages were distinguished. This constitutes a safe method allowing accurate classification prior to electron microscopic examination of the seminiferous epithelium.

Animals↗
PubMed1982

Ultrastructure of the "separating zone" of rat submandibular gland striated duct cells.

The separating zone of rat submandibular gland striated duct cells were studied in detail by electron microscopy. In order to get optimal stimulation of the glands the animals were starved for 24 h followed by feeding 15 min before sacrifice, and tissue removal. The separating zone is morphologically defined by numerous filaments which are located within the apical part of cell. Some of the filaments had a parallel arrangement, running horizontally and being attached to belt desmosomes at the cell periphery. Following the shedding of apical blebs, these filaments appeared to constitute the luminal boundary of striated duct cells. It was observed that the separating zones showed different degrees of density. This is supposed to reflect different stages in the reconstruction of the apical cell surface. It is thus considered that the separating zone, with numerous filaments, is of importance both of the apical secretion per se, and the formation of the new apical cell membrane.

Animals↗
PubMed1982

Comparison of craniofacial morphology in infants with incomplete cleft lip and infants with isolated cleft palate.

The craniofacial morphology was compared in 30 infants with incomplete left-sided cleft lip and 30 infants with isolated cleft palate. Cephalometric radiographs were obtained prior to any surgical management at 2-3 months of age in the lateral, anteroposterior and axial projections. Compared to the cleft lip group, the infants with isolated cleft palate showed the following characteristics: short anterior cranial base, short maxilla, reduced posterior maxillary height, reduced dimensions of the mandible--especially mandibular length--, narrow naso- and oropharyngeal airway. The cleft lip group had larger interorbital width and symmetry deviations in the anterior art of the maxilla. It was concluded that already at two months of age and prior to any surgical interference significant differences in craniofacial morphology distinguished the group with clefts of the secondary palate from the group with clefts of the primary palate.

Cephalometry↗
PubMed1982

[Velour prosthesis in fractures and luxations in the clavicular region. A follow-up study].

Twelve patients were followed up 6-40 months after implantation of a velours prosthesis due to trauma involving the clavicle. On the X-rays of the ten patients with a coracoclavicular loop there was a marked bone erosion in the area where the graft crossed the clavicle, and three patients had clinical symptoms as well. The use of a coracoclavicular velours prosthesis calls for a careful clinical and radiographic follow-up, and an early second operation for total or partial removal of the prosthesis if signs of bone erosion occur.

Clavicle↗
PubMed1981

Stage-dependent variations in volume density and size of Sertoli cell vesicles in the rat testis.

Membrane-bounded spherical vesicles found in rat Sertoli cells have been examined quantitatively during the cycle of the seminiferous epithelium. Most of the vesicles were localized to the basal and columnar portions of the Sertoli cell cytoplasm. The thin lateral projections of the Sertoli cells contained very few vesicles. Morphometric analysis of the basal portion of the Sertoli cell cytoplasm revealed that the volume density (Vv) of the vesicles changed markedly during the cycle. The Vv was at its minimum (0.036) at stage VII and maximum (0.117) at stages XI-I. The vesicles were also smaller at stage VII compared to the vesicles at stages IX-V. The stage-dependent differences in the size of the vesicles was found both in the basal and the columnar portions of the Sertoli cells. At stage VII some of the vesicles appeared to be elongated much like the tubular elements of the smooth endoplasmic reticulum (SER) from which they are probably derived. The stage-dependent differences in volume density and size in the Sertoli cells vesicles may be related to cyclic biochemical variations in the Sertoli cells, and are further indications of a variation in Sertoli cell function during the cycle of the seminiferous epithelium. Whether or not this is due to an "internal" cycle of the Sertoli cell or to influences from adjacent germ cells remains to be determined.

Animals↗
PubMed1981

Angiography after temporary inhibition of blood flow followed by intraarterial 5-FU infusion in the treatment of liver metastases.

Temporary inhibition of the blood flow to the liver was performed in 20 patients with metastases by tightening slings around the hepatic artery for 16 hours. Infusion of 5-FU started two days later through a catheter in the gastroduodenal artery. The response to the treatment as revealed by angiography was swelling of the liver and necrosis of the metastases. Thirteen patients responded favourably to the treatment. Complications such as abscess formation, 5-FU leakage, thrombosis of the hepatic artery and development of aneurysm at the entry of the infusion catheter occurred in some patients.

Adult↗
PubMed1981

Ossification defects and craniofacial morphology in incomplete forms of mandibulofacial dysostosis. A description of two dry skulls.

The morphology of two East Indian dry skulls exhibiting anomalies which were suggested to represent incomplete forms of mandibulofacial dysostosis is described. Obvious although minor ossification anomalies were found localized to the temporal, sphenoid, the zygomatic, the maxillary and the mandibular bones. The observations substantiate the concept of the regional and bilateral nature of the malformation syndrome. Bilateral orbital deviations, hypoplasia of the malar bones, and incomplete zygomatic arches appear to be hard tissue aberrations which may be helpful in examination for subclinical carrier status. Changes in mandibular morphology seem to be less distinguishing features in incomplete or abortive types of mandibulofacial dysostosis.

Adult↗
PubMed1981

A comparison of occlusions in two groups of children whose clefts were repaired by three different surgical procedures.

An inter-center comparison of the types and degrees of malocclusion found in children with complete unilateral cleft lips and palates is reported. The children were operated upon at two Scandinavian cleft palate centers, where three different surgical procedures were used. The sample was comprised of 47 Danish and 55 Norwegian children in either the primary or early mixed dentition stage. Malocclusions were documented by the method of Björk et al. (1964) prior to any orthodontic treatment. Deviations in transverse and incisor occlusion were tabulated and compared. Differences were found in the frequency of crossbite of the buccal teeth on the cleft side and in the distribution and frequency of anterior crossbite. The differences are discussed in relationship to the different methods of surgical management.

Child↗