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Biomedical subjects

E Bauer

Publications and source records attributed to E Bauer.

At least 163 records · Page 9Linked to original sources
PubMed1989

Use of injectable cultured human fibroblasts for percutaneous tissue implantation. An experimental study.

Large quantities of cultured human facial dermal fibroblasts were propagated from randomly selected patients to determine their relative suitability as percutaneously injectable living implants. Volumetric and histologic comparisons were made between the following implants that were injected subcutaneously into athymic nude mice: (1) cultured human fibroblasts (HFb); (2) cultured human fibroblasts dispersed in Zyderm II collagen (HFb + Zyd); (3) Zyderm II collagen (Zyd); and (4) Zyplast collagen (Zyp). Both the HFb and HFb + Zyd implants were accepted as primary takes but regressed volumetrically at significantly greater rates than either the Zyd or Zyp implants. Correlative immunohistochemical staining revealed that, by 10 days, 90% of the cells within the HFb implants and 80% within the HFb + Zyd implants were of human origin; however, by 9 weeks, approximately 25% of the cells were of human origin in both types of implants. These results indicated that cultured human fibroblasts can be successfully injected as living grafts; however, the subsequent gradual attrition in the numbers of implanted cells, as noted in this model system, limits the long-term retention of the implants.

Animals↗
PubMed1989

ESWL management of ureteral calculi without anesthesia: an alternative to invasive procedures.

We reviewed our experience with ESWL therapy in 96 patients who presented with upper, middle and lower ureteral calculi; we had 127 treatments in 96 patients, 73 men (76%) and 22 women (24%). All the treatments were performed without general anesthesia and without premedication. The average number of shock waves was 3.000. Repetition of the ESWL therapy had to be performed in 25%.

Adolescent↗
PubMed1989

[Use of direct calorimetry for continuously following the course of experimental infections in the mouse].

To obtain information about early symptoms of infection measurements of heat production were performed in experimentally infected animals. As a model Mengo virus-infected mice were used. Construction and physical properties of the flow calorimeter device are described. Fever was not detected by method used. However, deviations from normal behaviour of the animals were demonstrated as a result of the relationship between motoric activity and heat produced. Furthermore, the method may be particularly useful for the determination of the exact time of death, which is of interest for mathematical modelling of survival data (RECKNAGEL et al. 1986; GUTHKE et al. 1987; SUHNEL und VECKENSTEDT 1989).

Animals↗
PubMed1989

[Results and complications of treatment of acute and chronic pleural empyema--a retrospective analysis of the last 5 years].

In 162 patients treated surgically for pleural empyema, 212 surgical interventions were necessary. As primary treatment cest-tube drainage was more effective than closed chest tubes. Decortication, which was carried out in 43 patients, was followed by further surgical procedures in three patients. If primary treatment is not effective early decortication is indicated to avoid septic complications (30-day mortality amounted to 7.4%). Two patients died from septic complications.

Chronic Disease↗
PubMed1988

Influence of disposable ('Conchapak') and reusable humidifying systems on the incidence of ventilation pneumonia.

The contamination of disposable ('Conchapak') and reusable humidifying systems and their influence on the incidence of pneumonia was studied in 116 patients requiring continuous mechanical ventilation therapy. The water reservoirs of 11 (15.9%) of the 69 disposable systems became colonized, but all reusable systems were found to be sterile. In four of the 11 samples, the organisms isolated corresponded with those cultured from tracheal secretions several days before. Ventilator-associated pneumonia occurred in 36 (31.0%) of the patients, but there was no statistically significant difference in the incidence of pneumonia between the patients treated with the disposable or the reusable humidifying systems. Gram-negative bacteria were the predominant organisms isolated from tracheal aspirates of patients who developed ventilator-associated pneumonia. These results suggest that disposable humidifying systems do not influence the rate of ventilator-associated pneumonia in mechanically ventilated patients.

Adult↗
PubMed1988

The use of endomyocardial biopsy in heart failure.

Endomyocardial biopsy in this study of 1250 biopsied patients (mean of five samples/patient) proved to be a remarkably safe technique with no lethal complications. It may help to detect the underlying cause of heart failure but is handicapped by sampling error in focal disease processes (such as myocarditis and sarcoid heart disease) when conventional light and electron microscopy are used. In this biopsy series 123 patients (9.8%) suffered from severe heart failure; lymphocytic infiltrates were found in only 10 (8%). Immunohistological data suggested a secondary humoral immunopathogenesis in all patients with myocarditis and perimyocarditis, in 75% of patients with postmyocarditic heart muscle disease and in 48% of patients with primary dilated cardiomyopathy. There may thus be a need for a new classification of heart muscle diseases that includes immunological parameters of humoral and cellular autoreactivity.

Biopsy↗
PubMed1988

Lack of detectable N-nitroso-N-methyl-N-cyclohexylamine in humans after administration of bromhexine.

The possible formation of N-nitroso-N-methyl-N-cyclohexylamine (NMCA) from the drug bromhexine (N-methyl-N-cyclohexyl-(2-amino-3,5-dibromobenzyl)-ammonium hydrochloride) and nitrite was investigated in humans using three different approaches: 1. analysis on metabolites of NMCA in human urine; 2. analysis on NMCA in human gastric juice; 3. in vitro incubation of human gastric juice with therapeutic bromhexine doses. Diet given to volunteers was varied during these investigations with respect to nitrate content. Experiments with a maximum load of 200 mg nitrate to stimulate nitrite formation were performed. Results of in vivo experiments did not indicate any formation of NMCA. In one out of 39 ex-vivo/in-vitro experiments (with a load of 100 mg nitrate in drinking water) 0.5 ng NMCA/ml gastric juice could be detected which is near the detection limit. Finally, this study showed that bromhexine is not secreted by saliva. This allows to conclude that nitrite and bromhexine do not reach the stomach simultaneously over a longer period of time. In consequence, medication with bromhexine is not regarded to represent a risk due to nitrosamine formation.

Bromhexine↗
PubMed1988

Penetration and permeation into human skin of fusidic acid in different galenical formulation.

The penetration into the horny layer and permeation through the skin of fusidic acid, incorporated in different bases, e.g., cream, ointment and a gel (Fucidine), was investigated in vitro in a penetration chamber, using human skin. Whereas the horny layer offers a marked barrier to penetration in normal skin, this barrier can be greatly reduced by damaging the horny layer by adhesive tape stripping. Fusidic acid showed penetration into the different layers of skin which was related to the duration of application, including the penetration chamber fluid, i.e., there was penetration of the entire skin thickness. In the presence of spontaneous changes in the horny layer, as in seborrhoeic warts and similar conditions, fusidic acid penetrated well into the deeper layers of the skin. Fusidic acid is thus a substance which permeates better in regions where the horny layer is damaged, and where it can attain high levels, which in practice are antimicrobial. Penetration into the skin is thus a function of damage to the horny layer and the quality of the horny layer itself. From this point of view the amount of absorption in dermatoses depends on the area of skin with damage to the horny layer. Penetration through normal skin can be disregarded.

Fusidic Acid↗
PubMed1987

[Malformations (duplications) of the terminal ileum in adults. Report of 3 unusual cases].

Three cases of rare malformations of the ileo-cecal region (duplications) are discussed and compared to 20 others of the same kind already published. The main characteristics are an inflammatory symptomatology, especially in the right hypogastrium, with periodic recurrences. Since the clinical, radiological, endoscopic and biopsy findings often simulate Crohn's disease, patients must frequently undergo years of unsuccessful medical treatment and innumerable follow-up examinations. Nevertheless, when certain findings are taken into consideration they can lead to correct diagnosis of this rare disease, which is established through pathological anatomy. The special features of the disease are described in detail. It can be definitively cured through resection of the ileo-cecal segment.

Adult↗