Biomedical subjects

E B Lane

Publications and source records attributed to E B Lane.

147 records · Page 9Linked to original sources

PubMedDate not supplied

Co-expression of vimentin and cytokeratins in parietal endoderm cells of early mouse embryo.

Of the five classes of intermediate filaments found in vertebrate tissues, the cytokeratins are considered unique to epithelial tissues, while vimentin is expressed by endothelial and mesenchymal cells. In neither case is the precise function of the filament system known. Epithelial cells in culture often express vimentin as well as cytokeratins, but co-expression in vivo, as reported for pleomorphic adenomas of the parotid gland and metastatic carcinoma cells in ascites or pleural fluid, is still controversial. Here we report the co-expression of cytokeratins and vimentin in situ, in the parietal endoderm of the mouse embryo 8.5-13.5 days old. This population of individual, motile cells seems to be derived from a conventional epithelium by migration and differentiation. Our results support the idea that vimentin expression is specifically related to reduced cell-to-cell contact, and to the independent existence of a cell following detachment from an epithelial sheet.

Animals↗

PubMedDate not supplied

Exfoliative cytology of normal oral mucosa. Assessing the basal cell keratin phenotype.

OBJECTIVE: To attempt to evaluate the likelihood that normal keratin (antibody LH8) expression might contribute to a false positive diagnosis when sampling a lesion affecting the oral mucosa. STUDY DESIGN: Smears were taken from four oral sites (buccal mucosa, hard palate, dorsal tongue, ventral tongue) in 28 patients using the Axibrush. These were processed using a standard avidin-biotin technique, and the number of brown "positive" cells was assessed. RESULTS: None of the smears contained cells that were LH8 positive. CONCLUSION: While the results of this study suggest that exfoliative cytology is poor at sampling the basal cells of intact oral epithelium, it does indicate that the identification of LH8-positive cells in a smear from a clinically suspicious lesion is unlikely to be due to harvesting of normal basal cells.

Antibodies, Monoclonal↗

PubMedDate not supplied

Lung cancer-associated keratin 19 fragments: development and biochemical characterisation of the new serum assay Enzymun-Test CYFRA 21-1.

From a panel of 4 murine monoclonal antibodies directed against keratin 19 various antibody combinations were evaluated in solid-phase enzyme-linked sandwich immunoassays for detection of soluble keratin 19 fragments in patient sera. One of these antibody combinations, comprised of the monoclonal antibodies Ks 19.1 and BM 19.21, was selected for further development to a routine test (Enzymun-Test CYFRA 21-1) because of its high diagnostic sensitivity and specificity for non-small cell lung carcinoma (NSCLC). Both antibodies are specific for keratin 19, no reactivity could be observed with cytokeratin 8 or 18. The epitopes of the two antibodies were determined to be within helix 2B of the rod romain. The epitope sequences lie within the sequence 311-335 for the catcher antibody Ks 19.1 and 346-367 for the detector antibody BM 19.21. These sequences are unique, as could be confirmed from sequence databases. The standard material for the assay was prepared from a cytoskeleton fraction of cultivated MCF-7 cells. Subsequent digestion of this fraction with chymotrypsin yielded a soluble and stable standard material. Both the standard material and the serum analyte appeared as oligomers when analysed on gel chromatography: the serum analyte appeared exclusively at a M(r) of 100 +/- 10 kD, whereas the standard material eluted in fractions corresponding to 100 +/- 10 kD and 450 kD. Due to the precise definition of the antigen and the localisation of the antibody binding sequences, Enzymun-Test CYFRA 21-1 is one of the best characterised tumor markers so far.

Animals↗