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Biomedical subjects

E Apostoloff

Publications and source records attributed to E Apostoloff.

At least 37 records · Page 2Linked to original sources
PubMed1987

The influence of interferon-gamma, interleukin-2, prostaglandin E2, and cyclosporine on the polyclonal and anti-DNA antibody secretion in lymphocyte cultures derived from patients with systemic lupus erythematosus.

The influence of various immunoregulatory substances was studied in lymphocyte cultures derived from patients suffering from systemic lupus erythematosus (SLE) by using the model of spontaneous secretion of polyclonal immunoglobulin G (IgG)/immunoglobulin M (IgM) and anti-DNA autoantibodies. Compared with healthy donors, lymphocytes derived from patients with active SLE disease showed an elevated secretion of total IgG as well as anti-DNA-IgG in vitro, which was associated with an increase in the proportion of activated (HLA-class II +) T cells in their peripheral blood. Recombinant interferon-gamma increases the total IgG/IgM as well as anti-DNA-IgG/IgM secretion, which suggests that it has a possible role in the pathogenesis of SLE disease. Recombinant interleukin-2 and prostaglandin E2 normalize the high, spontaneous total IgG secretion, but elevate anti-DNA-IgG/IgM secretion. These results suggest that autoreactive B-cell clones are regulated differently in SLE patients. Cyclosporine inhibits total IgG/IgM secretion in all patients and anti-DNA-IgG/IgM secretion in six of eight patients. The possible therapeutic use of such immunomodulatory substances in SLE disease is discussed.

Adolescent↗
PubMed1987

[Atrophic corpus gastritis and autoimmune gastritis].

The authors tried to clarify relations between autoimmune gastritis and isolated atrophic corpus gastritis by bioptic corporal and antral examinations from 150 probands as well as examinations of gastrin in serum and parietal cell antibody tests. Only 30% of all patients examined with isolated atrophic gastritis of the corpus part revealed criteria of an autoimmune gastritis. Therefore investigations of antibodies against parietal cells are necessary to mark off both clinical pictures. This differentiation seems to be necessary regarding the high risk of gastric cancer following an autoimmune gastritis.

Autoantibodies↗
PubMed1986

A sensitive and class specific solid phase enzyme immunoassay for anti-DNA autoantibodies in supernatants of lymphocyte cultures and human hybridomas.

A solid phase enzyme immunoassay using methylated bovine serum albumin (BSA)-precoated and DNA-coated microtiter plates was developed for the detection of IgG and IgM anti-DNA autoantibodies in the supernatants of lymphocyte cultures and hybridomas. In patients with systemic lupus erythematosus (SLE) the significantly raised IgG anti-DNA antibody synthesis indicates that preactivated anti-DNA clones circulate in the peripheral blood. This was associated with the detection of anti-DNA antibodies in the sera. The screening of 53 supernatants from human x mouse hybridomas showed an antibody to denatured DNA in 16 supernatants.

Antibodies, Monoclonal↗
PubMed1986

[Course control and significance of circulating HBsAg-specific immune complexes in acute viral hepatitis B].

The occurrence and behaviour of circulating HBsAg-specific immune complexes were investigated in 52 patients suffering from acute viral hepatitis B. In 6 patients the illness took a chronic course. The determination was carried out by precipitation with polyethylene glycol 6,000 and the increase of HBsAg concentration after antibody splitting from the complexes by trypsin treatment. The results allow following conclusions: The early presence of immune complexes suggests a specific antibody response also against HBsAg before development of clinical illness. The timing and strength of this humoral immune reaction is sex dependent; females show anti-HBs earlier and in higher titers. The course of the concentration of circulating immune complexes depends on the immunoglobulin class of antibody and the quickness of virus elimination. The determination of circulating unspecific or HBsAg-specific immune complexes does not allow to draw any prognostic conclusion to the further course of acute hepatitis B. Only in chronificating cases, the future activity of the chronic HBV infection may be expected by the early behaviour of HBsAg immune complexes. Also patients developing chronic course of the HBV infection demonstrated a "peak" of the immune reaction 6-8 weeks after the beginning of disease, which could offer the point of time for starting an immunomodulating therapy.

Adolescent↗
PubMed1983

[Demonstration of circulating immune complexes using a C1q-solid phase enzyme immunoassay].

An enzyme-immunoassay for the quantitative determination of circulating immune complexes in human serum is described. The technique uses alkaline phosphatase-conjugated anti-human IgG to detect immune complexes bound to solid phase C1q. This assay is sensitive detecting 4 ng/ml to 60 micrograms/ml aggregated IgG. First data from normal individuals and patients with systemic lupus erythematosus show that this method is specific and sensitive for detection of circulating immune complexes.

Antigen-Antibody Complex↗
PubMed1983

[Parietal cell antibodies and stomach cancer].

Sera of 101 patients with histologically confirmed gastric cancers were investigated for parietal cell antibodies, which are the serological markers of chronic atrophic gastritis type A. These autoantibodies were more often found in patients with early than with advanced gastric cancers. They were more frequent in intestinal than in diffuse gastric cancers. It is discussed that in advanced cancers the frequency of parietal cell antibody is diminishing because of loss of antigene or binding of antibodies in immune complexes. Early gastric cancers therefore seem to be more suitable than advanced cancers to study the relation between gastric cancer and gastritis type.

Antigen-Antibody Complex↗
PubMed1981

[ds-DNA- and denaturized ds-DNA auto-antibodies in old age and their pathological determination (author's transl)].

The antibodies of 50 old people of the age over 70 years were determined against ds-DNA and denaturized ds-DNA by the immune-fluorescence method, and it can be stated that denaturized ds-DNA antibodies are traceable in 16% of these sera. Such antibodies could not be found in control tests of seras of young people. However, the old people did not show may clinical symptom typical of the disease caused by these antibodies. In particular, there were no indications of an auto-immune disease, for that reason, these antibodies are to be considered as optional pathological ones.

Aged↗