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Biomedical subjects

E Alessi

Publications and source records attributed to E Alessi.

At least 109 records · Page 6Linked to original sources
PubMed1979

[Cutaneous mycobacteriosis of aquaria. A disease transmitted from fish to man].

The Authors refer about one case of cutaneous infection from Mycobacterium marinum transmitted to man from tropical fishes of a fish tank. The Authors describe the lesions produced by mycobacterium in man and in fish, as well as the relevant hystological findings and the microbiological enquiries carried out for isolation and typing. The possibility of direct transmission of a tubercular infection from fish to man has been demonstrated. The Authors think it to be useful a careful sanitary control on the ichthyc fauna destined to domestic fish tanks.

Animals↗
PubMed1978

[Syphilis serology: "ELISA", a third generation test].

The characteristics and the working modalities of ELISA (Enzyme Linked Immunosorbent Assay) are outlined at the onset. Subsequently, the Authors refer the data relevant to the use of ELISA in the syphilis serodiagnosis, by describing the operative phases for the working out of the test with the enzyme peroxidase, and reporting the results of the clinical investigation performed on primary and secondary syphilis in progress, on treated primary and secondary syphilis (examinations performed 1 to 10 years after the end of therapy), on healthy blood donors, and on samples of false biological positivities. On the basis of the analysis of the results, the Authors feel that ELISA for its characteristics of sensitivity, easiness in carrying out, rapidity and reproducibility, might find a rational indication in the modern serodiagnosis of the syphilis.

Blood Donors↗
PubMed1978

TPHA test. Experience at the Clinic of Dermatology, University of Milan.

The results of serological investigations with the treponemal haemagglutination (TPHA) test are reported in a large number of luetic and non-luetic patients. From these the following conclusions can be drawn: (1) The sensitivity of the TPHA test is decreased if the test is carried out with micromethods; however, in our opinion, the same degree of sensitivity can be obtained either with the macromethod at dilution 1/80-1/60 or with micromethods at 1/20-1/40. (2) The sensitivity of this test is high, being similar to that of the fluorescent treponemal antibody absorption (FTA-ABS) test in primary and secondary syphilis and even higher than that of the FTA-ABS test in treated subjects. (3) The specificity of the reaction is high, as demonstrated by examining sera in patients with a negative history and clinical examination together with negative results to Treponema pallidum immobilisation (TPI) and FTA-ABS tests, and by studying biological false positive sera. (4) For the serological screening, it may be sufficient to perform the TPHA test with the 1/20-1/40 micromethod together with the Venereal Disease Research Laboratory (VDRL) test. In patients with suspected syphilis, it is advisable to perform the TPHA test by the macromethod, in combination with the FTA-ABS test.

Antibodies, Bacterial↗
PubMed1978

[Observations on the sequence of endocardial activation in the left ventricle of the normal subject].

To obtain information on normal left ventricular activation, endocardial recordings with an electrode catheter were made a seven left ventricular sites in ten patients undergoing diagnostic heart catheterization. All the patients had: 1) sinus rhythm; 2) normal duration and shape of the QRS complex of left chest leads; 3) normal left ventriculography, i.e. normal volume and contractility of the left ventricle. The earliest left ventricular endocardial activation was recorded at septal and/or posterior level, i.e. at the septum (6 to 16 msec, average 9.7 msec, after the onset of intracardiac QRS complex) in seven patients; at the posterior wall (0 to 4 msec, average 2.6 msec, after the onset of intracardiac QRS complex) in three patients (in one of these, the earliest activation occurred at the posterior wall and apex simulaneously). If the earliest activation occurred at the left interventricular septum, the next excited point was found on the posterior wall or at the apex, and vice versa. The latest part to be activated was on the lateral free wall in seven patients; on the posterior wall in two patients; at the apex in the last one.

Adult↗
PubMed1978

[Comparative study of five different methods for measuring urinary porphyrins (author's transl)].

The AA. have compared five different methods for measuring urinary porphyrins (Bio-Rad porphyrins, Porphyrin-T Sibar, Reinkingh-Van Kampen, Schwartz and coll., Fischer-Ham). Based on the results obtained, for total porphyrins the best method appeared to be the Porphyrin-T Sibar test, while for the separate evaluation of uro- and coproporphyrins the most suitable method proved to be the one proposed by Reinkingh-Van Kampen. The investigations has also allowed the AA. to conclude that spectrophotometric reading can replace the fluorimetric one, without affecting the accuracy of results.

Coproporphyrins↗
PubMed1977

[Treponemal hemagglutination test (TPHA). Validity of the micromethod using dilutions of sera 1/20-1/40].

The Authors studied the possibility to use, for the carrying out of the TPHA micromethod, higher serum dilutions, as preliminary investigations had pointed out an insufficient sensitivity of the reaction performed at the 1:80 - 1:160 dilutions, particularly in the primary-secondary phase of the luetic infection. The Authors controlled, preliminarily, that at the dilutions studied, no unacceptable increase of spontaneous hemagglutinations and false positive reactions took place in the test sera. The investigation stressed the acceptability of the methodological modification set forth.

Hemagglutination Tests↗
PubMed1976

[The sexual transmission of Australia antigen (HBsAg)].

Examination was made of 300 serum specimens taken from patients with latent syphilis, in order to study the incidence of HBsAg antigen as compared with the normal population. Examination was by means of counter immunoelectrophoresis, radioimmunoassay and electron microscope with negative staining. The incidence as shown by counter immunoelectrophoresis is 2.33%, by RIA 10% with 86.6% of sub-type AY and 13.3% of sub-type AD, which is significantly higher than the incidence in the normal population. These data would therefore seem to confirm the possibility of sexual transmission of hepatitis B.

Antibody Formation↗
PubMedDate not supplied

Treatment of gonorrhea with thiamphenicol.

For many years thiamphenicol has been the drug of choice for the treatment of uncomplicated gonorrhea at the Center for Venereal Disease of the University of Milan. During the last four years, 1,110 of 1,112 cases of uncomplicated gonorrhea treated with 500 mg of oral thiamphenicol three times a day for six days have been cured. Recently, the effectiveness of single-dose treatment of uncomplicated gonorrhea with this drug was evaluated. A dose of 2.5 g of thiamphenicol was given orally to 159 patients. The diagnosis of gonococcal infection was based on culture results in all cases. In 77 cases red and white blood cell counts and hemoglobin and serum iron determinations were made before and one week after therapy. Clinical and bacteriologic cure was achieved in 144 (90.6%) of the 159 patients. The only adverse reactions to the drug were transient diarrhea in ten patients, epigastric pain in two, and vomiting in one. No statistically significant variations were detected in blood tests.

Anal Canal↗
PubMedDate not supplied

Monoclonal antibody direct immunofluorescence for the identification of Neisseria gonorrhoeae strains grown on selective culture media.

Commercially available reagents for monoclonal antibody immunofluorescence, polyclonal antibody immunofluorescence, and coagglutination were employed in comparison with a rapid sugar fermentation test used for identifying clinical isolates of Neisseria gonorrhoeae recovered on modified Thayer-Martin media. Of 68 gonococcal strains, 68 were positive by the monoclonal immunofluorescent antibody. Of 16 nongonococcal gram-negative, oxidase-positive diplococci, all were negative by this reagent. Monoclonal antibody immunofluorescence was more specific and more sensitive than polyclonal antibody immunofluorescence and more sensitive than coagglutination.

Antibodies, Monoclonal↗
PubMedDate not supplied

Mycoplasma hominis and Ureaplasma urealyticum. Comparison of media for isolation and prevalence among patients with nongonococcal nonchlamydial genital infections.

One hundred and twelve genital specimens were cultured for Mycoplasma hominis and Ureaplasma urealyticum using a conventional system and the Mycotrim GU biphasic culture system. Both systems gave the same percentage of recovery (60%) of Mycoplasma hominis, while the recovery rates of Ureaplasma urealyticum were 95% with the conventional system and 83% with the Mycotrim GU. The media contamination was similar for both systems. The average time for Mycoplasma isolation and identification was shorter with the Mycotrim GU in comparison with the conventional system. The prevalence of Mycoplasma hominis in 91 patients with nongonococcal nonchlamydial urethritis and/or cervicitis was 8%, that of Ureaplasma urealyticum was 31%.

Cross-Sectional Studies↗