Search PubMed⌕ Search

Biomedical subjects

E Adam

Publications and source records attributed to E Adam.

At least 145 records · Page 8Linked to original sources
PubMed1983

A micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: parameters involved in standardization.

A micro solid-phase radioimmunoassay (micro-SPRIA) was developed to demonstrate type-specific antibodies to herpes simplex virus types 1 and 2 (HSV1 and HSV2). Glycoproteins from the 123,000 dalton region of HSV1 (VP123) and the 119,000 dalton region of HSV2 (VP119) were isolated on preparative polyacrylamide gels for use as antigens in the micro-SPRIA. Human sera selected from clinical samples by virological history and appropriate microneutralization data were used to standardize the micro-SPRIA. Optimization of the assay required the use of siliconized microtiter wells for adsorption of antigen. Maximized results were highly dependent on the concentrations of antigen, primary antibody, and secondary antibody as well as the diluents used for these principal test reagents. Incorporation of HSV glycoproteins of each respective type with the optimal condition established in this study facilitates the direct detection of type-specific antibody in human sera.

Antibodies, Viral↗
PubMed1983

A micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: specificity and sensitivity.

The specificity and sensitivity of a micro solid-phase radioimmunoassay (micro-SPRIA) that detects type-specific IgG antibody to herpes simplex virus types 1 and 2 (HSV1 and HSV2) were evaluated. Glycoproteins VP123 (molecular weight, 123,000) of HSV1 and VP119 (molecular weight, 119,000) of HSV2 were found to display the greatest degree of antigenic type-specificity of several HSV antigens tested with the micro-SPRIA technique. When testing a group of sera, negative for anti-HSV antibodies by microneutralization, in the micro-SPRIA, a range of negative reactivities was noted, suggesting that cut-points should be determined for each antigen preparation. The micro-SPRIA detected appropriate antibody activity in patients with recurrent infection and a marked agreement was noted in comparison to detection of anti-HSV antibodies measured with the microneutralization test. The type-specificity of the micro-SPRIA was substantiated by the independence of test results using VP119 and VP123 antigens for a random group of positive sera. The assay is rapid, specific, and sensitive and allows the testing of multiple serum samples with a standardized set of reagents.

Antibodies, Viral↗
PubMed1983

Frontiers of nursing in the 21st century: development of models and theories on the concept of nursing.

In the past nursing has used a medically oriented perspective; consciously or not, nursing practice, education and research have been guided by the same conceptual frame of reference as has medicine. For nursing to justify its claim to being an independent health profession offering a particular service to society, it must adopt its own conceptual base, one that indicates those phenomena that are of concern to nursing and those health problems that nursing must try to solve. Many nurses have already chosen to base their teaching, research and nursing care on one of the existing conceptual models for nursing. The challenge for the 21st century is that all nurses adopt an explicit conceptual base. Broader than a theory, a conceptual model specifies nursing's focus of inquiry and may thus lead to the development of theories which will prove useful not only to nurses but to other health professionals as well. Since nursing exists to provide a necessary service to mankind, its conceptual base must be evaluated by using specific social criteria.

Models, Theoretical↗
PubMed1983

The maintenance of rat metrial gland explants in vitro, in the presence and absence of progesterone.

Explants of metrial gland were taken from uteri of rats at day 13 of pregnancy and maintained in vitro for up to 9 days. Some cultures were supplemented with progesterone. The progress of the explants was monitored by light and electron microscopy. In the absence of progesterone, large amounts of collagen were apparent and many cells contained lipid inclusions. The granulated cells which are characteristic of the gland were not seen after 4 days in culture. In the presence of progesterone, though apparently normal granulated cells were readily found after 4 days in culture and could be identified after 9 days, some showed changes which do not occur in vivo. Collagen accumulation and the development of lipid inclusions were less extensive in the presence of progesterone.

Animals↗
PubMed1982

Kinetics of human antibody responses to primary genital herpes simplex virus infection.

5 patients with primary genital herpes simplex virus (HSV) infection were studied prospectively for the production of serum antibody reactivity as measured by the virus micro-neutralization test (NT), micro-solid phase radioimmunometric assay (micro-SPRIA), and antibody-dependent cellular-cytotoxic (ADCC) test. ADCC antibody was detected before reactivity measured by either NT or micro-SPRIA in 2 of the patients. A 3rd patient failed to produce neutralizing antibody, whereas specific activity was detected by the other two methods. In 2 other patients a good correlation was observed for development of antibody by all three methods. There were several discordant results in the kinetics of production of antibody detected by these assays, suggesting that not all individuals produce similar types of antiviral antibody.

Adult↗
PubMed1982

The role of herpes simplex virus in cervical and vulvar cancer.

This report extends the association of herpes simplex virus type 2 (HSV2) with cervical cancer to vulvar carcinoma. In addition to seroepidemiologic observations linking prior HSV2 infection to genital cancer, the new evidence involves the neoplasms themselves. Biopsies of cervical and vulvar cancers were found to contain HSV2-specified antigens. The virus-induced antigens are nonstructural DNA-binding proteins normally present in the nuclei of infected cells; however, in the cancer cells they were found in the cytoplasm. Whole virus structural antigens were not detected in the biopsies. In recent years there has been a parallel rise in prevalence of HSV2 infections and vulvar carcinomas, particularly in women under 40 years of age, reaffirming the association of the virus infection and the neoplasms.

Animals↗
PubMed1982

Development of clear cell adenocarcinoma in DES-exposed offspring under observation.

Two cases of clear cell adenocarcinoma of the vagina detected at follow-up in young women exposed in utero to diethylstilbestrol are reported. One patient, aged 23, had been followed for 2 years before carcinoma was diagnosed; the second patient, aged 22, had been seen on a regular basis for 5 years, 8 months. In both instances, suspicion of the presence of carcinoma was aroused by the palpation of a small nodule in the vaginal fornix. Hysterosalpingography was performed on both patients and, in 1 instance, an abnormal x-ray film was reflected by the gross appearance of the uterine cavity found in the surgical specimen.

Adenocarcinoma↗
PubMed1982

Carcinoma in situ of the vulva. The search for viral particles.

Biopsy specimens taken from eight women with squamous-cell carcinoma in situ of the vulva were studied with transmission electron microscopy for the presence of viral particles. In one of the specimens a 150-nm-diameter viruslike particle was found. The exact nature of this particle was not clear, but it could have represented an immature herpesvirus particle. In seven specimens no evidence of viral particles was detected. These findings provide further evidence that the positivity of vulvar carcinoma in situ for herpesvirus antigen is not due to the presence of whole virus particles.

Adult↗
PubMed1981

Herpesvirus-induced antigens in squamous-cell carcinoma in situ of the vulva.

Antigens induced by herpes simplex virus Type 2 (HSV2) were found to be associated with squamous-cell carcinoma in situ of the vulva in nine of 10 patients. The HSV2-induced antigens are DNA-binding proteins that are normally present in the nuclei of infected cells, but in the cells of the carcinomas in situ they were found in the cytoplasm. Whole-virion structural antigens were not present, although there was serologic evidence of previous HSV2 infection in patients tested for the presence of antibodies. The observations reported here and the recent parallel rise in the prevalence of both HSV2 infections and vulvar carcinoma in situ, particularly in women under 40 years of age, suggest an association of HSV2 infection with this type of neoplasia, the nature of which remains to be determined.

Adult↗
PubMed1981

Recurring laryngeal papillopa.

A prospective study of 31 children born of mothers with condylomata acuminata at parturition or during pregnancy was undertaken to test the hypothesis that laryngeal papilloma could be associated with venereal warts and could be acquired perinatally. None of the children born of mothers with condylomas at delivery or during their pregnancies had developed symptoms, signs, or observable lesions of laryngeal papilloma, whether delivered vaginally or by cesarean section. Nevertheless we do not know the true incidence of this disease or the infectivity of the causative micro-organism. Although it appears that the correlation between laryngeal papilloma in children and the presence of condylomata acuminata in the mother during pregnancy or parturition is not as strong as our retrospective study had suggested, it should not be disregarded.

Adolescent↗
PubMed1980

Asymptomatic virus shedding after herpes genitalis.

Samples of cervical and vaginal secretions obtained daily from five women were studied for the presence of active virus and/or viral antigens. In women with a history of recurrent genital herpes, either infectious virus or viral antigen in exfoliated cells was repeatedly detected in the absence of overt clinical symptoms. The epidemiologic and obstetric importance of the observations is discussed.

Antigens, Viral↗
PubMed1980

Upper genital tract changes and pregnancy outcome in offspring exposed in utero to diethylstilbestrol.

Hysterosalpingography (HSG) was performed on 267 diethylstilbestrol (DES)-exposed women. One hundred eighty-five (69%) of these women demonstrated an abnormality and 82 women had normal findings. A relationship between abnormal x-ray findings and structural abnormalities of the cervix and vaginal epithelial changes was noted. Ninety-three women on whom roentgenography was performed had a total of 144 pregnancies. The pregnancy outcome in this group of women was significantly worse than that found in a control population of 87 women who had 147 pregnancies. In addition, women with abnormal x-ray films were found to have a poorer pregnancy outcome than the group of women with normal findings. The women with abnormal x-ray films had more ectopic pregnancies, spontaneous abortions, and premature deliveries than women with normal x-ray films although these differences were not statistically significant. Significantly fewer women with abnormal findings than women with normal x-rays were delivered of term infants.

Abortion, Spontaneous↗