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Biomedical subjects

D Schlesinger

Publications and source records attributed to D Schlesinger.

At least 37 records · Page 2Linked to original sources
PubMed1985

Multiple non-repeated epitopes on the circumsporozoite protein of Plasmodium knowlesi.

The Plasmodium knowlesi circumsporozoite (CS) protein contains a repetitive immunodominant epitope. Here we show that the serum of rabbits repeatedly immunized with P. knowlesi sporozoites contains antibodies which bind to immobilized synthetic peptides ('C2', 'N2', and 'charged') representing two different polar regions of the CS polypeptide. These reactions are specific since the binding is inhibited only by the homologous peptides. Antisporozoite antibodies were isolated from the rabbit serum by affinity chromatography on Sepharose beads coupled to two synthetic peptides, 'C2' and 'charged'. Both purified antibodies recognized the CS protein and the intracellular precursors as shown by Western blotting analysis using sporozoite extracts. These results demonstrate that the corresponding areas of the native CS molecule are immunogenic, accessible to interaction with antibody, and therefore constitute potential targets for vaccine development. In addition, the present findings confirm the published amino acid sequence of a large portion of the CS protein which has been deduced from the nucleotide sequence of the corresponding gene.

Amino Acid Sequence↗
PubMed1985

UMPK polymorphism in the Polish population.

Studies on UMPK polymorphism were carried out in a sample of the Polish population numbering 462 subjects. The occurrence of three phenotypes was confirmed--UMPK 1, UMPK 2-1 and UMPK 2. From distribution of phenotypes, frequencies of determining genes were calculated with the following results: UMPK1-0.9762, UMPK2-0.0238, The usefulness of this system in paternity cases is 1.69%.

Electrophoresis, Starch Gel↗
PubMed1985

Population studies on PLG group system in the Polish population.

Distribution of PLG types was studied in a sample of the Polish population numbering 230 subjects by the method of high-voltage agarose electrophoresis. Of three phenotypes encountered, PLG1, appeared with the frequency of 0.4870, PLG2-1 with 0.4391 and PLG2 with 0.0739. Assuming the hypothesis of PLG controlled by 2 alleles the frequencies of genotypes were calculated as follows: for PLG1 gene -0.71 and for PLG2 gene -0.29. PLG system in the Polish population was confirmed to be in a state of genetic equilibrium. The frequencies of PLG genes in the Polish population do not deviate from the frequencies encountered in other European populations.

Blood Group Antigens↗
PubMed1985

Activity of erythrocyte alanine aminotransferase in the Polish population.

Alanine aminotransferase activities were tested in erythrocytes of Polish population sample of 213 adults (105 women and 108 men) and 95 children (aging 6 months to 4 years). In the tests a modified method of Wróblewski and Cabaud was used. A significant scatter of enzyme activities was noted, which ranged in adults between 0.76 and 36.86 mU/ml. In all studied groups, GPT 1 type individuals showed the highest and GPT 2 type donors the lowest activity of the enzyme. Mean activity of GPT 1 type adults was 19.27 mU/ml, in GPT 2-1 adults 13.6 mU/ml and in GPT 2 adults 8.87 mU/ml. GPT 2 type activity corresponds to approximately 46% and GPT 2-1 type activity to, approximately, 71% activity of GPT 1 type. Independently of GPT type, activity of the enzyme was lower in women in whom it amounted to 71-83% activity detected in men. In children, alanine aminotransferase activity was higher than in adults about 15-29%.

Adult↗
PubMed1984

PGM1 subtypes in the Polish population.

Frequency of PGM1 subtypes in the sample of the Polish population numbering 197 subjects was determined by the method of isoelectric focusing. Ten subtypes were encountered. Frequencies of genes were calculated from the subtype distribution with the following results: for PGM1+1 0.698, for PGM1-1 0.058, for PGM2+1 0.180 and for PGM2-1 0.064.

Gene Frequency↗
PubMed1983

Levels of corticotropin releasing factor-like immunoreactivity in mammalian hypothalamic and extrahypothalamic brain tissue as determined with a monoclonal antibody to the ovine material.

A monoclonal antibody to ovine corticotropin releasing factor (CRF) has been produced by fusion of a non-producing plasmacytoma cell line P3U1 with spleen cells of Balb/c mice immunized with the synthetic 41 amino acid peptide coupled covalently with rabbit myosin by a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio) propionate. A total immunizing dose of 500 micrograms resulted in a highly specific, high-affinity antibody with a Ka of 0.15 x 10(12) M-1, which was used to establish a specific RIA with a sensitivity of 10 pg/tube. Levels of corticotropin releasing factor-like immunoreactivity (CRF-LI) in a pg/mg of hypothalamic tissue ranged from 4-10 in ovine, 2.5-8 in bovine, 47.5-67.5 in mouse and 2.3-20 in human tissue. Moreover, CRF-LI was widely distributed in extrahypothalamic mouse brain at concentrations approximately one half those seen in hypothalamus.

Amygdala↗
PubMed1983

Distribution, biosynthesis, and physiological role of corticotropin-releasing factor in the human: an overview.

Our findings to date indicate that: A peptide resembling oCRF is present in human and mammalian hypothalamus. oCRF is present in human lumbar cerebrospinal fluid. oCRF concentrations do not differ in CSF from normal individuals and from patients with Cushing's syndrome. oCRF appears to be synthesized via a large oligopeptide precursor. An oCRF-like molecule (oCRF-LI) is present in hypothalamic brain tissue. We have also observed more tentative evidence of low levels of oCRF-LI outside of the brain. oCRF is likely to be a central mediator of stress in its multiple forms. We believe that oCRF is clearly of major physiological importance, but that many critical unanswered questions remain. Probably, the most fascinating of these, which we are only beginning to comprehend, concerns the functions of CRF in extrahypothalamic brain as well as the CRF which appears to be present outside the brain.

Animals↗
PubMed1982

Complete amino acid sequence of urotensin I, a hypotensive and corticotropin-releasing neuropeptide from Catostomus.

Urotensin I, purified from extracts of the urophysis of a teleost fish (Catostomus commersoni), exhibits potent hypotensive activity (mammals and birds) and corticotropin-releasing activity (both fish and mammals). The primary structure of this 41-residue peptide was determined to be H-Asn-Asp-Asp-Pro-Pro-Ile-Ser-Ile-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Asn-Met-Ile-Glu- Met-Ala-Arg-Ile-Glu-Asn-Glu-Arg-Glu-Gln-Ala-Gly-Leu-Asn-Arg-Lys-Tyr-Leu-Asp-Glu -Val-NH2. Extraction with 0.1N HCl at 100 degrees C cleaves the amino-terminal tripeptide, yeilding a fully active analog, urotensin I(4-41). The amino acid sequence was confirmed by measuring the biological activity of synthetic urotensin I(4-41). Urotensin I exhibits a striking sequence homology with ovine corticotropin-releasing factor and with frog sauvagine. These three peptides exhibit similar activities in biological test systems.

Amino Acid Sequence↗
PubMed1981

Phagocytosis-promoting activity of avian plasma and fibroblastic cell surface fibronectins.

The purpose of this investigation was to determine whether fibronectin preparations from both chicken plasma and cell surface of fibroblasts can promote phagocytosis of gelatin-coated latex particles. Chicken plasma fibronectin was isolated (a) by ammonium sulfate fractionation, chromatography on Sepharose-4B followed by purification on a Sepharose-4B-heparin column; (b) by affinity chromatography on a Sepharose-4B-rat-antifibronectin column; (c) by affinity chromatography on Sepharose-4B-gelatin followed by molecular sieve separation on Sepharose-CL4B; (d) by a dual affinity chromatographic method using a Sepharose-4B-gelatin column and a Sepharose-4B-heparin column. Chicken cell surface fibronectin from fibroblast cultures was purified by ammonium sulfate precipitation followed by chromatography on Sepharose-CL4B. The purity of preparations was examined by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate; all samples showing high purity. The opsonic activities of the preparations were measured by the uptake of 125I-labeled gelatin coated latex particles in conjunction with rat liver slice, and peritoneal macrophage monolayer systems. Both the plasma fibronectin and cell surface fibronectin preparations showed substantial opsonic activities in the test systems. Fresh chicken plasma did not reveal any phagocytosis promoting activity due to the presence of some unidentified inhibitor(s). The results showed that an opsonically active protein can be isolated from chicken plasma or serum and this protein is identical to plasma fibronectin. Furthermore, it could be concluded that cell surface fibronectin from chicken fibroblasts also can serve as an opsonin for gelatin coated particles.

Animals↗
PubMed1981

Hereditary of the three enzyme systems EsD, Gt and GLO in the Polish population. The family studies and mother/child combinations.

Hereditary of the three enzyme systems EsD, Gt and GLO was studied in families and mother/child combinations. EsD types were determined in 88 families with 197 children and 1813 mother/child combinations. Gt types were studied in 78 families with 174 children and 114 mothers with one child. GLO types were determined in 82 families with 180 children and 298 mother/child pairs. The segregation in the different family combinations is in accordance with the simple formal model of two alleles at the autosomal loci.

Adult↗
PubMed1979

Polymorphism of esterase D in the Polish population.

Distribution of esterase D types was studied in a sample of the Polish population numbering 885 subjects. Frequency of EsDl-1 type was 0.810, EsD2-1 -- 0.174, and EsD2-2 -- 0.16. Frequency of the genes EsD1 and EsD2, which determine this system, were 0.897 and 0.103 respectively. A study of 613 mother-child pairs revealed agreement with the hypothesis of two alleles in the EsD locus. The prevalence of EsD genes in the Polish population was similar to those reported in other European populations.

Alleles↗
PubMed1979

Prevalence and inheritance of C3 types in the Polish population.

In a sample of the Polish population numbering 1,458 subjects, the three common types, C3S, C3F and C3FS, were found with frequencies of 0.6790, 0.0295 and 0.2901 respectively, besides two rare variants, C3FO, 7S and C3SO, 25S. Frequencies of C3S and C3F genes were 0.8248 and 0.1746 respectively, and of the CSFO,7 and C3SO,25 genes, 0.0003 each. Distribution of types in 695 mother-child pairs was consistent with the hypothesis that the C3 system depends on a single genetic locus in which codominat alleles are situated. No children of C3S and C3F mothers with opposite homozygous types were encountered. Hereditary character of the C3FO, 7S variant was confirmed by family studies in which the rare C3FO,7 gene, in combination with C3S or C3F genes, occured in three generations.

Alleles↗
PubMed1978

Frequency of Gt types in the Polish population.

The genetic polimorphism of galactose-1-phosphate-uridyl-transferase was studied in the sample of Polish population including 133 subjects. Three phenotypes were found, Gt 1-1 with a frequency of 0.8722; Gt-2-1 0.1203 and Gt 2-2 with a frequency 0.0075. Gene frequencies were Gt1 0.932 and Gt2 0.068.

Alleles↗
PubMed1978

GLO polymorphism in Polish population.

The determination of GLO types was carried out using two methods: on high voltage agarose gel and cellulose acetate foil. In the sample of the Polish population including 201 persons, three GLO types were encountered with the following frequencies: GLO 1-1 0.2438, GLO 2-1 0.4477, GLO 2-2 0.3035. Frequencies of GLO1 gene was 0.4726, GLO2 0.5724.

Erythrocytes↗
PubMed1978

Types of serum proteins and erythrocyte enzymes in rheumatoid patients.

Four group systems of serum proteins (Hp, Gc, Km, Gm) and five group systems of erythrocyte enzymes (AP, PGM1, GPT, AK, EsD) were determined in samples of patients with rheumatoid arthritis and in healthy controls. Statistically significant differences were found in Gm system, namely Gm(1) factor was more frequent in rheumatoid patients than in healthy subjects.

Acid Phosphatase↗
PubMed1978

Group systems of serum proteins and erythrocyte enzymes in malignant lymphoma.

Four group systems of serum proteins (Hp, Gc, Gm, Km) and five group systems of erythrocyte enzymes (AP, PGM1, GPT, AK, EsD) were determined in 63 patients with malignant lymphoma. Statistical analysis of the distribution of the above mentioned systems in patients and Polish population samples did not reveal any significant differences, which points to the lack of any correlation between the disease and the group systems under examination.

Acid Phosphatase↗