The indirect haemagglutination test was used for serological type identification of Pasteurella strains. The Pasteurella multocida strains were tested by parallel application of antigen production methods (common method plus hyaluronidase treatment). Thirty-nine (72.2 per cent) of 54 Pasteurella multocida strains, which had been obtained from cattle and swine, were serologically tested by the common technique of antigen production, their types being identified, while 42 (77.7 per cent) were tested and typed by means of hyaluronidase treatment. Depolimerisation of hyaluronic acid by means of hyaluronidase increased the agglutinability of eight strains (14.8 per cent) which could thus be identified by types. Serological identification of 47 (87.1 per cent) Pasteurella multocida strains was achieved by parallel use of the two antigen production methods. Identification was not possible of two strains. Cross-reaction was recorded from five strains. Most of the Pasteurella multocida strains isolated from cattle were A serotype (54 per cent), while those of swine were B strains (50 per cent). Thirty-six of 40 serologically tested Pasteurella haemolytica strains were serotypes A1, A2, T3, T4, A5, and A6, and most of these were A5 and T4.

Animals↗

PubMed1980

[Quantitative makeup of the gastrointestinal flora of piglets in the 1st 2 weeks of life. 2. The development of the gastrointestinal flora in primary specific-pathogen-free piglets].

Quantitative germ flora analyses were applied to 22 SPF piglets, aged between four hours and 14 days, to follow up the development of their gastro-intestinal flora. The development of a fairly stable gastro-intestinal flora was delayed. Escherichia coli strains were isolated from SPF piglets which had died following diarrhoea and septicaemia, but they did not fall in those E. coli sero-groups known for pathogenicity to swine. The outcome of raising was greatly stabilised and amounted to about 70 per cent by administration of something between 80 ml and 100 ml of radiation-sterilised sow colostrum.

Aging↗

PubMed1980

[Development and prophylactic use of Escherichia coli-smd live vaccine in swine].

This live vaccine was officially accepted and registered in 1976. Based on streptomycin-dependent mutants, it can be efficiently used for prophylaxis against enterotoxaemia and, consequently, help in stabilising production. The vaccine has to be administered subcutaneously to animals aged between two and three weeks and on the day of weaning. Tolerability is localised and generally good. However, differences in effectiveness, so far unexplained, were found to exist between different stocks. The loss of animals was reduced in all stocks which had been prophylatically immunised. The vaccine sould be considered as an intermediate step for immunoprophylaxis against coli-enterotoxaemia.

Animals↗

PubMed1980

[Recommendations for isolation and differentiation of Pasteurella multocida].

Explore the source record for details and available documents.

Animals↗

PubMed1980

[Indirect detection of biological substances with endotoxin activity by serotonin liberation from thrombocytes].

Explore the source record for details and available documents.

Animals↗

PubMed1979

[Studies of bovine mycoplasma mastitis. 1. Review of literature on the occurrence of bovine mastitis with Mycoplasma involvement].

The clinical pattern as well as the pathologico-anatomic or histological changes due to mycoplasma mastitis are neither specific nor pathognomic. Mastitis pathogens so far described included M. bovis, M. bovigenitalium, A. laidlawii, A. axanthum, M. alkalescens, M. canadense, M. dispar, M. bovirhinis, strains of Group 7 according to Leach, strain ST 6, and ureaplasma strains. In the GDR, enzootic mastitis has been confined to A. laidlawii and A. axanthum.

Animals↗

PubMed1979

[Studies of bovine Mycoplasma mastitis. 2. Testing of various culture media and culture methods for the isolation of Mycoplasma from milk samples].

The recipes Medium-I broth, Medium-B broth, and Weissenseemycoplasma broth as well as Medium-I agar, Medium-B agar, and MRL agar were tested for their applicability to culturing mycoplasma from milk samples, using the direct and indirect techniques. No dependable information on the occurrence of mycoplasma was obtainable from tested material unless several nutritive media and techniques were combined. Medium I, for which almost no imported substances were needed, was in no way inferior to common international nutritive substrates. Its use in conjunction with the indirect culturing technique is recommended for routine diagnosis.

Animals↗

PubMed1979

[Studies of bovine Mycoplasma mastitis. 3. Testing of biochemical properties of Mycoplasma strains isolated from 3 stocks].

Thirty-two isolates of altered milk samples taken from cows with mastitis on three industrialised dairy cattle units were biochemically tested following five passes through no-inhibitor media, assessment of filtratability through 450-nm-membrane filter, and clone assay. Something between five and ten clones of each of the strains involved were tested for their growth properties both at 22 degrees C and in no-serum media, sensitivity to digitonin and capability of aesculin hydrolysis. TTC reduction (triphenyltetrazolium-chloride), as well as for decomposition of glucose, arginine, and urea. The properties found were characteristic of M. bovis, A. laidlawii, and A. axanthum, and of the family of mycoplasmataceaea in the strains of one of the stocks.

Animals↗