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Biomedical subjects

D Radu

Publications and source records attributed to D Radu.

63 records · Page 4Linked to original sources
PubMedDate not supplied

Some aspects of cellular and humoral immunity in bronchial asthma and allergic rhinitis.

Blood lymphocyte subpopulations were investigated in 50 patients with allergic diseases (38 with allergic rhinitis and 12 with allergic bronchial asthma). The total T lymphocyte (Tt) count (E rosette test), the active T lymphocyte (Ta) count (active E rosette test) and the B lymphocyte count (EAC rosette test) were determined and the results were correlated with: 1) lymphocyte sensitization in vitro to different allergens and to PPD; 2) circulating immune complexes; 3) serum histaminopexic capacity. In allergic rhinites a decrease of Tt and B lymphocytes and an increase of Ta lymphocytes was observed. In allergic bronchial asthma the Tt lymphocyte were found reduced. Lymphocyte reactivity to PPD in vitro was present in 100% of the cases and in vitro lymphocyte sensitivity to several allergens was observed in most of the cases. The histaminopexic capacity was absent or low in 75% of the patients with allergic rhinitis and in 66.6% of the patients with allergic bronchial asthma. The involvement of cellular mediated immunity in allergic diseases is discussed.

Adult↗
PubMedDate not supplied

Circulating immune complexes in chronic hepatitis as related to the presence of HBsAg.

The relationship between the presence of circulating immune complexes (CIC) and of HBsAg in the serum of patients with chronic liver diseases was studied in a group of 78 patients of whom: 16 with chronic persistent hepatitis (CPH), 39 with chronic active hepatitis (CAH) and 23 with liver cirrhosis (LC). The results showed a clear relationship between the incidence of CIC and the presence of HBsAg in the patients with persistent or active chronic hepatitis but not in those with liver cirrhosis. The presence of HBsAg was detected in the composition of CIC in only part of the cases (10/25 patients). This low incidence suggests that in chronic hepatitis other antigens can also participate in the formation of CIC.

Antigen-Antibody Complex↗
PubMedDate not supplied

Test of leukocyte migration inhibition. II. Comparative study of the techniques in capillary tubes and in agarose.

The object of the paper was to estimate the advantages and disadvantages of two techniques of the leukocyte migration inhibition (LMI) test (in capillary tubes and in agarose) for the in vitro testing of delayed type hypersensitization. The capillary technique was carried out according to Soborg and Bendixen and the agarose technique according to Clausen. The antigen used in both techniques was PPD in a concentration of 10 micrograms/ml. The techniques were performed in parallel in two groups of individuals: 15 healthy controls and 20 patients with chronic hepatitis. It was observed that in the control group sensitization to tuberculin in vitro was detected by the capillary technique in 7 of the 15 subjects (47%) and by the agarose technique in 4 of the 15 subjects (27%). In chronic hepatitis sensitization to PPD was detected, by the capillary method, in 11 of the 20 patients (55%) and by the agarose method, in 9 of the 20 patients (45%). The results were consistent by the two methods in 18 of the 20 patients. In conclusion the LMI test in capillary tubes is preferable to that in agarose being simpler, more rapid, more sensitive and less expensive.

Adolescent↗
PubMedDate not supplied

Active rosette-forming lymphocytes in chronic hepatitis.

Lymphocyte subpopulations (total T lymphocytes, active T lymphocytes, late T lymphocytes, B lymphocytes), leukocyte migration inhibition test, serum HBsAg, serum immunoglobulins (IgG, IgA, IgM) were determined in 15 chronic active hepatitis patients and 35 chronic persistent hepatitis patients (diagnosed clinically, biochemically and histologically) and 10 healthy subjects who had acute B viral hepatitis three years ago. A decreased number of total T lymphocytes has been found in HBsAg-seropositive chronic hepatitis. An increase of relative and absolute number of active T lymphocytes was noticed in HBsAg-seronegative healthy persons with a past history of acute viral hepatitis B.

Acute Disease↗
PubMedDate not supplied

Procain and diethylaminoethanol influence on the release of free oxygen radicals by polymorphonuclear leukocytes, in rabbits and humans.

The investigations were conducted on 3 groups of New Zealand rabbits: 1) controls; 2) injected with procain, i.m. 15 mg/kg body weight, daily, for 30 days; 3) injected with diethylaminoethanol (DEAE), 15 mg/kg body weight, daily, for 35 days. The study was made also on human leukocytes, isolated from the peripheral blood of 10 clinically healthy subjects (adults), procain and DEAE action being investigated in vitro. The free oxygen radicals (FOR) released by PMN leukocytes were evaluated by chemiluminescence, in vitro. Addition of procain or DEAE had no effect on the release of FOR by PMN leukocytes of control rabbits. In the experiment made on rabbits treated with procain or DEAE, the release of FOR by PMN leukocytes was much more reduced, as compared to controls. In the rabbits treated with procain, the intensity of the emitted light was 2.27 mV, in those treated with DEAE, 3.46 mV, while in the controls, the mean value was 6.74 mV. In the in vitro experiments performed on human PMN cells stimulated with opsonized zymosan (OZ), addition of procain or DEAE had an inhibiting effect on the FOR release. As compared to control, the means of the FOR values decreased from 59 to 41.2 mV in case of procain addition and from 67.7 to 50 mV in case of DEAE addition. The fact that the inflammation is associated with accumulation of free radicals, suggests the opportunity to test these substances, especially DEAE, as antioxidant agents.

Anesthetics, Local↗