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Biomedical subjects
Publications and source records attributed to D Hunter.
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Nine calves were inoculated with tissue cysts of a low virulence strain of Toxoplasma gondii and killed at intervals up to 10 weeks. An immunological response took the form of circulating antibodies and reactive changes in the lymph nodes. The organism could only be recovered from the lymph nodes and this only during the first four weeks after infection. No tissue cysts were demonstrated and only a few inflammatory lesions occurred. Thus, although calves can be infected, the immunological response is prompt with rapid elimination of the organism.
This experiment investigates the holding property of polyglycolic acid suture in repair of divided dog sciatic nerves and the influence of absorption of this synthetic material on regeneration. The conclusion is that PGA maintains its tensile strength sufficently long to allow both physical union and good regeneration of the divided nerve. The suture absorption does not influence regeneration.
The aim of this experiment was to investigate the reaction of peripheral nerve tissue to a synthetic absorbable suture material. Polyglycolic acid suture material was investigated by means of electron photomicrographs. It was concluded that placement of polyglycolic acid into the peculiar environment of endoneurial tissues results in minimal scarring and in minimal disturbance of the surrounding nerve fibers. The material was progressively absorbed with minimal disturbance of intrafascicular structures.
A direct fluorescent antibody technique for the detection of Treponema hyodysenteriae in faeces and bowel smears from pigs produced a better preparation than the indirect method and also had the advantages that it took less time, produced less non-specific fluorescnece and gave direct control over the availability of the reagents used.
The radioassay of very small 125I samples in both conventional and side-hole well crystals has been investigated. Using a multichannel analyzer, the source was counted at various positions within these well crystals. The effect of using a single-channel pulse-height analyzer was simulated by summing the counts over a 24-keV range across the primary peak, over a 30-keV range across the sum-coincidence peak, and over a 56-keV range across both peaks. In all crystals the primary peak was independent of sample position within the well over a range of several centimeters. The contribution of the sum-coincidence peak varied significantly with sample position in the conventional well crystals. With the side-hole crystal the counts contributed by the sum-coincidence peak exhibited a 2-cm plateau.
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