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Biomedical subjects

D H Rice

Publications and source records attributed to D H Rice.

At least 91 records · Page 5Linked to original sources

Detection of cytomegalovirus from clinical specimens in centrifugation culture by in situ DNA hybridization and monoclonal antibody staining.

An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). However, the earliest that CMV could be detected by DNA hybridization was 58 h as compared with 16 h with monoclonal antibodies following centrifugation. DNA hybridization remains of great interest for the study and detection of CMV infection. However, current DNA hybridization techniques are not sufficiently rapid to replace the use of monoclonal antibodies in centrifugation culture.

Antibodies, Monoclonal↗

Evaluation of a latex particle agglutination assay for the detection of cytomegalovirus antibody in patient serum.

The Virogen CMV Antibody Test is a simple and rapid latex agglutination assay for the detection of cytomegalovirus antibody in human serum and plasma. Evaluation of this assay with respect to enzyme immunoassay yielded a sensitivity of 98% with a specificity of 100%. In comparison to CMVScan, the Virogen CMV Antibody Test had a sensitivity of 98.4% and a specificity of 100%.

Antibodies, Viral↗

Contractile properties of canine thyroarytenoid muscle reinnervated from the ansa cervicalis.

In an attempt to obtain data on the contractile properties of vocal fold muscle reinnervated from the ansa cervicalis, we severed the recurrent nerve and connected its distal stump to the ansa cervicalis by an end-to-end anastomosis in a series of dogs. Each dog was allowed to heal for 5 months. Then the section of the thyroid cartilage on which the vocal fold muscle inserted was detached, connected to an isometric force transducer, and activated by indirect stimulation successively on both the operated and unoperated sides. A series of twitch contractions was recorded from each side. In two dogs no contractile response was obtained. In the three others, the twitch contraction time was increased significantly, by 23% to 60%. In two of three dogs, the operated muscle was significantly weaker than the unoperated muscle. The reinnervated thyroarytenoid muscle changed its speed in the direction of the donor sternothyroid muscle.

Anastomosis, Surgical↗

Blood transfusions and recurrence in head and neck cancer.

Recent reports in the general surgery literature have shown decreased survival with blood transfusions in abdominal cancer surgery. We reviewed our experience with 100 consecutive patients, all of whom had 5-year follow-up, to see if this held true for head and neck cancer. The detailed report covers site and stage. In summary, however, the recurrence rate for all cancers of the larynx was 14% for those who did not receive blood and 65% for those who did. For cancer of the oral cavity, pharynx, and nose or sinus, the recurrence rate was 31% without transfusions and 71% with transfusions. It would appear that the use of blood transfusions somehow adversely affects the patient's survival. Further study needs to be done to elucidate the mechanism.

Adult↗

Endoscopic sinus surgery: results at 2-year followup.

Endoscopic sinus surgery is a relatively new technique for the management of sinusitis refractory to noninvasive therapy. Success depends on the use of newly developed instruments, including the endoscopes, that allow one to enlarge the natural drainage ostia of the involved sinuses. Furthermore, its use assumes that the mucosal disease is reversible and that destructive procedures, such as the Caldwell-Luc, are unnecessary. To date there have been no reports in the English literature of patients followed for more than a few months. Herein we report 100 consecutive patients followed for at least 2 years.

Adolescent↗

Extraskeletal myxoid chondrosarcoma of the head and neck: a case report.

Extraskeletal myxoid chondrosarcomas are rare, soft tissue tumors with a characteristic histologic appearance. The majority of these tumors occur in the extremities. To our knowledge, this is the first reported case in the head and neck. As a group, extraskeletal myxoid chondrosarcomas behave in a less aggressive manner than do other forms of chondrosarcomas, but their clinical course can vary considerably. Surgery is the treatment of choice, and adjuvant radiotherapy or chemotherapy should be considered for cases that display a more cellular pattern and a less conspicuous myxoid matrix.

Chin↗

Basic surgical techniques and variations of endoscopic sinus surgery.

This article gives complete information on the care of the endoscopic sinus surgery patient. Indications and preoperative evaluations necessary for endoscopic sinus surgery are described. The technique of functional endoscopic sinus surgery is covered in detail. Postoperative care and complications are also described. Various procedures for patients with extensive or unusual disease are discussed.

Adult↗

Difficult decisions in endoscopic sinus surgery.

Sinus disease, like many other problems in otolaryngology, may run the gamut of being either easily correctable with a clear-cut treatment or completely resistant to any form of treatment. Fortunately, many patients have problems somewhere between these two extremes. Clearly there is no one correct approach for treatment of many problems. Two, three, or even four approaches may be equally effective in some cases. In other cases, one approach may be successful in the hands of one surgeon whereas another approach is more appropriate for a different surgeon. This article presents nine cases that do not have clear-cut solutions. In some, the consultants clearly disagreed, whereas in others there was some uniformity in treatment plans. This flexibility in surgery in general and specifically with a technique that is relatively new is what makes it so exciting and so promising for continued success in treating sinus disease.

Adult↗

Presentation and management of neck abscess: a retrospective analysis.

A retrospective analysis of deep neck abscesses managed at the Los Angeles County-USC Medical Center was performed. Fifty-one patients met the criteria and were reviewed as to presentation, etiology, location, and microbiology. Hemolytic streptococci and anaerobic species, especially Bacteroides and peptostreptococci, were the most common organisms isolated. Intravenous drug abuse was the most common etiology, and was an important source of primary carotid space infection. This group was most commonly infected with Streptococcus species (50%).

Abscess↗

Magnetic resonance imaging of the salivary glands. A comparison with computed tomographic scanning.

A variety of diagnostic imaging techniques are currently available for the investigation of parotid gland disorders. At this time, for the investigation of mass lesions, computed tomography is the most valuable. Over the past several years, we have been comparing magnetic resonance imaging with computed tomographic scanning of our parotid gland lesions to evaluate the relative merits of both systems. This current study illustrates our current experience and shows the relative strengths and weaknesses of both methods.

Adult↗

Acute bacterial sinusitis and diabetes mellitus.

Diabetes mellitus has deleterious effects on the immune system which may lead to infection that is more serious and difficult to treat. Fifteen diabetic patients with acute bacterial sinusitis are reviewed. Diabetic pathophysiology and its relationship to infection are discussed. A protocol for treatment is outlined, with emphasis on the importance of intravenous antibiotics.

Adult↗

Bell's palsy and HLA-DR. A possible association.

No association has been found between HLA-A,B, or HLA-C antigens and Bell's palsy. We performed HLA-DR typing on 30 patients with Bell's palsy. Our results suggest possible associations with HLA-DR2, -DR4, -DRW6, and -DRW7 antigens. A significant association between HLA-DR and Bell's palsy may indicate a genetic basis for susceptibility to this disease.

Chromosome Mapping↗

Xerostomia. A treatment feasibility study in dogs.

An acute study was performed in dogs to test the feasibility of using another bodily fluid in place of saliva in patients with xerostomia. Chyle was routed from the thoracic duct to the oral cavity via a vein graft using the external jugular vein. While technically challenging, the vein conduit worked well in delivering chyle to the oral cavity.

Animals↗

Malignant schwannoma of the cervical vagus nerve in a patient with neurofibromatosis (von Recklinghausen's disease).

A patient with malignant schwannoma arising from the cervical vagus nerve has been described. Treatment consisted of wide en bloc resection of the tumor and adjacent carotid artery. A subclavian-middle cerebral arterial bypass procedure, using a saphenous vein graft, was performed. A course of postoperative radiotherapy was administered. The patient remains without evidence of disease 11 months following therapy.

Adult↗

Snoring: clinical implications and treatment.

Snoring has only recently come under wide study. Recent research has established the close relationship of severe snoring to sleep apnea in terms of the obstructive pathophysiology. Snoring tends to increase in severity over time and may progress to sleep apnea. Severe snoring may be associated with pulmonary and systemic hypertension, secondary polycythemia, and cardiac arrhythmias.

Female↗

Evaluation of lasalocid as a coccidiostat in calves: titration, efficacy, and comparison with monensin and decoquinate.

Two experiments were conducted to evaluate lasalocid as a coccidiostat in Holstein calves and to compare lasalocid with monensin and decoquinate. In experiment 1, calves in 3 groups (6 calves/group) were each inoculated with 500,000 sporulated oocysts, 88% of which were Eimeria bovis and 12% were E zuernii. Calves in each group were given lasalocid-medicated feed at 0.50 (group 3), 0.75 (group 4), or 1 mg/kg (group 5) of body weight/day for 45 days. Two control groups (6 calves/group) were also evaluated; calves in control group 2 were inoculated and nontreated, and calves in control group 1 were noninoculated and nontreated. At 0.50, 0.75, or 1 mg/kg/day, lasalocid was equally effective in preventing induced coccidiosis (E bovis and E zuernii) in calves. Compared with inoculated nontreated controls, treated calves had significantly (P less than 0.05) fewer oocysts in feces and had fewer clinical signs of coccidiosis from days 16 to 30 after inoculation. Experiment 2 was conducted to compare the effectiveness of monensin, lasalocid, and decoquinate for the prevention of experimentally induced coccidiosis. Calves (n = 48) were allotted into 4 groups (12 calves/group); each was inoculated orally with 275,000 sporulated oocysts, predominantly E bovis and E zuernii, and each was given nonmedicated feed (group 6) or feed medicated with 33 mg of lasalocid (group 7), decoquinate (group 8), or monensin (group 9)/kg of feed for 46 days. Calves given medicated rations had significantly (P less than 0.05) fewer oocysts in their feces and fewer clinical signs of coccidiosis than did calves given nonmedicated rations.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

Difficult decisions in parotid surgery.

Six atypical case presentations of parotid tumors are presented along with solutions proposed by two experts on the topic. The differences and similarities of the opinions expressed are summarized and discussed by the editor.

Adenoma↗