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D Goldberg

Publications and source records attributed to D Goldberg.

318 records · Page 18Linked to original sources
PubMedDate not supplied

Phosphorylation of the S. cerevisiae Cdc25 in response to glucose results in its dissociation from Ras.

In the yeast Sacchromyces cerevisiae, addition of glucose to starved cells triggers a transient rise in the intracellular level of cyclic AMP that induces a protein phosphorylation cascade. The glucose signal is processed by the Cdc25/Ras/adenylyl cyclase pathway, where the role of Cdc25 is to catalyse the GDP-GTP exchange on Ras. The molecular mechanisms involved in the regulation of the activity of Cdc25 are unknown. We report here the use of highly selective anti-Cdc25 antibodies to demonstrate that Cdc25 is a phospho protein and that in response to glucose it is hyperphosphorylated, within seconds, by the cyclic AMP-dependent protein kinase. It is also demonstrated that, concomitantly with hyperphosphorylation, Cdc25 partially relocalizes to the cytoplasm, reducing its accessibility to membrane-bound Ras. These results are of general significance because of the highly conserved sequence of Ras-guanyl nucleotide exchange factors from yeasts to mammals.

Adenylyl Cyclases↗
PubMedDate not supplied

Surveillance for the impact in the UK of HIV epidemics in South Asia.

OBJECTIVES: To determine whether, because of the extensive recent spread of HIV infection in South Asia, South Asians (those people who classify themselves as Indian, Pakistani, Bangladeshi or Sri Lankan in origin) resident in the UK were at increased risk of HIV infection and to review current surveillance systems for detecting any such increase. DESIGN: Analysis of: ethnic grouping and probable country of infection recorded on voluntary confidential reports of AIDS cases and newly diagnosed HIV infections; blood donation testing data; reports of imported gonorrhoea infections; country of birth data from the unlinked anonymous (UA) survey of Sexually Transmitted Disease (STD) clinic attenders; district of residence data from the UA survey of pregnant women; ethnic grouping of prevalent diagnosed HIV infections. RESULTS: Few reported AIDS cases or HIV infections were found in people of South Asian ethnic origin and few reported HIV or gonorrhoea infections were associated with exposure in South Asia. Data derived from the UA programme suggested as yet no increase in HIV prevalence in either STD clinic attenders born in South Asia or in pregnant women resident in districts containing substantial numbers of ethnic South Asians. CONCLUSIONS: There was no evidence that South Asians resident in the UK are currently at greater risk of HIV infection than people of white ethnicity or, therefore, that south Asian heterosexuals are a group deserving priority in HIV prevention. However, as rapid spread of HIV infection is being recorded in the Indian subcontinent, continuous monitoring is necessary. This will be facilitated by improved collection of ethnic group information in all surveillance activities.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Reduction in needle sharing among community wide samples of injecting drug users.

Evidence of reduced levels of needle sharing among injecting drug users (IDUs) has largely been confined to IDUs attending needle exchanges or receiving treatment. In this paper we present the results of a serial cross-sectional study of needle sharing conducted in Glasgow using a multisite sampling strategy. Of the estimated 9400 IDUs in the city, 503 were interviewed in 1990 and 535 in 1991. The proportion of IDUs reporting injecting with, or passing on used needles and syringes in the last 6 months fell significantly as did the number of individuals from whom equipment was received or passed on to. The impact of this level of sharing has been limited in terms of HIV transmission; the prevalence of HIV among the 1990 sample was 2.0% and 1.1% for the 1991 sample. However, the fact that third of IDUs in Glasgow continue to inject, even occasionally, with used equipment gives cause for concern in view of the other pathologies known to be associated with poor injecting hygiene.

Adolescent↗
PubMedDate not supplied

Low and stable prevalence of HIV among drug injectors in Glasgow.

The first HIV-positive diagnosis among injecting drug users (IDU) in Glasgow was made in 1985. By the end of 1987 prevalence among IDU receiving voluntary attributable tests was 4.8%. Since 1990, an annual cross-sectional survey of HIV prevalence and risk behaviours among 500 current Glasgow IDU has provided a more representative sample. Anonymously-tested saliva samples obtained from respondents revealed prevalence rates of 1.8%, 1.2% and 1.0% in 1990, 1991 and 1992 respectively. Since 1987 a wide range of measures aimed at reducing HIV-related risk behaviour among IDU has been introduced and maintained in Glasgow. Against this background, there is evidence that IDU in the city have reduced their risk behaviours. The findings reported here suggest that implementing harm-reduction measures when prevalence is low may inhibit the rapid dissemination of HIV.

AIDS Serodiagnosis↗
PubMedDate not supplied

Comparison of four survey methods designed to estimate the prevalence of HIV among female prostitutes who inject drugs.

In Glasgow, during the period January 1990 to December 1992, 4 different methods were used to determine HIV prevalence among female prostitutes who inject drugs: the surveillance of prostitutes undergoing voluntary named tests, an unlinked anonymous survey of prostitutes attending a health care drop-in centre, a community-wide voluntary anonymous survey of injecting drug users including female prostitutes, and a voluntary anonymous survey of female prostitutes recruited in Glasgow's red-light area. HIV prevalence varied from 1.2 to 4.7% though overlapping confidence intervals indicated no significant differences. A comparison of each approach leads the authors to suggest that the former 2 methods which are relatively inexpensive should be conducted, if possible, on high risk groups such as prostitutes and injecting drug users on a continuous basis. If these indicate HIV rates which are stable and less than 5%, implementation of more sophisticated voluntary anonymous approaches need only be done every 3 to 4 years. However, if rates are unstable and/or greater than 5% then such surveys should be initiated more frequently, perhaps biannually.

Adult↗
PubMedDate not supplied

Teaching methods for use by psychiatrists in primary care settings.

If psychiatrists are to be effective teachers in primary care settings, they must use methods which entail active participation by general practitioners, use a taxonomy which is appropriate to the kinds of psychological illnesses seen by general practitioners, and be aware of the importance of somatic presentations of neurotic illness. Examples are given of three teaching methods using television equipment which promote active participation by the general practitioners.

Family Practice↗
PubMedDate not supplied

Prediction of special education placement from birth certificate data.

The overall goal of this research effort was to develop procedures for accurately identifying children at high risk for special education placement, based on information available at the time of birth. A file containing information on all births in New York City between 1976 and 1986 was matched against the 1992 BIOFILE, which contains information on all children enrolled in the New York City public school system in 1992. A matched file containing birth and school information on 471,165 children resulted from this process. Three sets of risk factors were derived from birth certificate data: parental, pregnancy-related, and child-related. Using these risk factors as independent variables, a survival analysis model was developed predicting special education placement for each of three major disability categories: learning disability, emotional disorder, and mental retardation. A model combining all disability categories was also developed. The significant predictors of special education placement were Medicaid payment for birth (a poverty indicator), unmarried status of mother, large family size, low parental education, a mother born in the United States, a low level of prenatal care, male gender, low birthweight, and a low Apgar score. Male gender was the strongest risk factor in all models. Examination of selected survival curves indicated that the predictive power of the models is substantial. The methodology described in this article can be used to identify at-risk children for whom screening and other early interventions, including preschool programs, may be appropriate.(ABSTRACT TRUNCATED AT 250 WORDS)

Apgar Score↗
PubMedDate not supplied

[Factors implicated in the "routes of care" in mental health].

We analyze in this paper the way in which different sociodemographic and clinical factors influence, in different health areas of Cantabria (Spain), the pathways taken for patients suffering from "new psychiatric illness". The work is part of a Multicentric International Research Project developed by the World Health Organization aimed at evaluating and improving the quality of mental health care in different centres of the world. The general pathway to mental health services, in Cantabria, is dominated by the general practitioner (54.3%) and by the "hospital doctor/medical specialist" (26.4%), been therefore similar to the one found in other Spanish speaking centres, like Granada and Cuba; the differences are, however, much larger with the one found in Mexico city. It is also, possible to observe in our data that the pathways are influenced by clinical factors, among which the more relevant one is one related to the characteristics of the psychopathology. Thus in general we found that two factors like, the presence of "psycho-organic" syndromes and the severity of the psychopathology appears to influence: i) the degree of complexity of the pathway, which is higher in the "psycho-organic" and in the more severe forms of psychiatric disorders; ii) the protagonist which assumes, in detriment of the general practitioner, other "helping agencies" which is also increased in the "organic" and more severe disorders. We also found that other aspects like: the act of establishing "contact" with services, the delay in the obtention of help, and the prescription of psychotropic medicines, were also influenced by different sociodemographic, clinical and service related variables.

Adolescent↗
PubMedDate not supplied

Coding region of segment A sequence of a very virulent isolate of IBDV--comparison with isolates from different countries and virulence.

We determined the sequence of the coding region of segment A, coding for the viral proteins (VPs) VP2, VP4, and VP3, of a very virulent (vv) infectious bursal disease virus (IBDV) isolated in Israel and named IBDVks. We compared the deduced amino acid sequences of the proteins of the new isolate with those of the same proteins from several IBDV isolates, as published in recent years. The amino acid sequences of VP3 and VP4 of the Israeli isolate were 1.9%-2.3% different from the sequences of their counterparts from classical strains. Thus, the stable region of VP2 of IBDVks was very similar (0-0.68% difference) to the same region of VP2 from vv strains from Europe and Japan but distinct from that of proteins from classical strains from Europe, the United States, and Australia (up to 9.42% divergence), showing that IBDVks is more closely related to the vv strains from Europe and Japan. We found that viruses isolated in recent years resemble each other more than isolates from the same areas isolated a few years earlier. Hence, IBDVks can be categorized in one group with vv new isolates from Europe and Japan. This group has been found to be distinct from new isolates in the United States and strains isolated before the IBDV epidemic during the late 1980s.

Animals↗