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D Germain

Publications and source records attributed to D Germain.

At least 55 records · Page 3Linked to original sources
PubMed1987

Expression of a B-lymphoid differentiation antigen (CD 19) on acute non-lymphoblastic leukaemia cells.

The expression of CD 19 (B4) surface antigen was investigated in 41 cases of acute myeloid leukaemia (AML), and 8 cases of acute undifferentiated leukaemia (AUL). Diagnosis was carried out by usual cytological and cytochemical means, and leukaemias were classified according to the FAB classification. CD 19 antigen is thought to be expressed on B lymphoid cells only. However, 2 cases out of 9 FAB M1, M2 and M3 subtypes were weakly stained (20 to 25%) by B4 antibody; 7 out of 11, and 17 out of 18 cases of M4 and M5 subtypes were markedly stained (25 to 70% of cells). 3 cases of AUL were also stained. Moreover, B4 expression was often correlated with expression of monocyte-associated antigens, as recognized by CRIS6, My9, KiM1 and UCHM1 antibodies. It is concluded that B4 antigen is probably expressed before B differentiation and is retained in early stages of monocytic differentiation.

Antigens, Neoplasm↗
PubMed1987

[Unusual increase in amniotic fluid alpha fetoprotein and trisomy 13].

From 10 observations of trisomy 13, 3 presented an elevated amniotic fluid alpha-fetoprotein level considered as unusual in 2 cases, superior to cut-off level in the other case. Macroscopic examination of the three fetus could not reveal a cause of AFP elevation, neural tube defect or abdominal wall defect. The authors discuss the role of an undetected abnormality such as minor scalp defect with very thin membrane and for one case false-negative result of Kleihauer test.

Adult↗
PubMed1986

Variations in morphological and immunological blast cell phenotype in a case of acute leukaemia with t(4;11) translocation.

A case of acute leukaemia with t(4;11) chromosomal abnormality in a 28-year-old woman is reported. At diagnosis, two blast cell populations were seen: 60% of the cells were small cells with lymphoid morphology, 40% were large cells with monocytic morphology. Cytochemical examination was consistent with acute myeloid leukaemia (peroxidase-positive in 10% of the cells), but surface markers were those of common acute lymphoblastic leukaemia (CALLA, B4, TdT-positive, but My7-, My9- and OKM1-negative). Five days after diagnosis, although the only treatment had been platelet transfusions, there was a change in morphological and immunological phenotype: 40% of the cells were lymphoid and 60% monocytic. Lymphoid markers were expressed in only 20-40% of cells, and myeloid markers appeared on up to 60% of cells. We conclude that t(4;11) leukaemia could originate in an undifferentiated progenitor cell, which can undergo further differentiation into lymphoblasts or monoblasts, and that we were able to observe this in vivo differentiation in our patient.

Acute Disease↗
PubMed1985

Chromosomal R-banding with a monoclonal antidouble-stranded DNA antibody.

A monoclonal anti-DNA antibody (HB2) specific for poly dG- poly dC nucleotides was used to stain metaphasic lymphocyte or amniotic cell human chromosomes. HB2 fixation was revealed using either a peroxidase- or a rhodamine-labeled anti-mouse immunoglobulin antiserum. The staining pattern of the chromosomes was dependent on the HB2 concentration: R-banding could be observed at high antibody dilution. Previous trypsinization of metaphasic preparations demonstrated a precise and reproducible typical R-banding independent of the HB2 concentration. This technique appears to be an interesting alternative to other R-banding procedures. The specificity of the antibody allows a better understanding of the biochemical mechanism of R-banding.

Amniotic Fluid↗
PubMed1985

[Phosphatidylethanolamine methylase and cyclic nucleotide phosphodiesterase activities in human B lymphoid hemopathies].

Phospholipid methylase and cyclic nucleotide phosphodiesterase activities were studied in human B lymphoïd hemopathies (51 patients: acute lymphoblastic leukemia, B lymphoma, chronic lymphocytic leukemia, hairy cell leukemia) and compared with activities in lymphoblastïd and Burkitt lymphoma cell lines and with normal B lymphocytes: methylase activity proved to be lower in ALL and high grade lymphoma and inversely related to the percent of cells in S phase state; the A/G ratio of phosphodiesterases was low in ALL and CLL and high in hairy cell leukemia and it was related to the percent of cells in S phase state.

3',5'-Cyclic-AMP Phosphodiesterases↗
PubMed1984

[Prenatal diagnosis of a de novo trisomy case 9q-47,XX,+9 del(q33----qter)].

A case of de novo trisomy 9q- (deletion q33----qter) in utero diagnosed is reported. Features of this syndrome are described, compared with those of trisomy 9p et total trisomy 9. The possibility of prenatal diagnosis and the similarity of some features with nail-patella syndrome are focused.

Chromosome Aberrations↗
PubMed1983

[Chromosome instability syndromes].

Chromosome instability syndromes are defined by either an increase of chromosomal breakage or by an increase of sister chromatid exchange number, or by an increase of the two. Bloom's syndrome, Ataxia telangiectasia, Fanconi's Anemia are the main components of this group. The incidence of cancers or malignant blood diseases is high. The finding of DNA repair abnormalities in some of them and their high sensitivity to particular mutagenic agents make these syndromes an interesting model for oncogenesis.

Anemia, Aplastic↗
PubMed1982

[Chromosome instability syndromes].

Chromosome instability syndromes are defined by either an increase of chromosomal breakage or by an increase of sister chromatid exchange number, or by an increase of the two. Bloom's syndrome, Ataxia telangiectasia, Fanconi's Anemia are the main components of this group. The incidence of cancers or malignant blood diseases is high. The finding of DNA repair abnormalities in some of them and their high sensitivity to particular mutagenic agents makes those syndromes an interesting model for oncogenesis.

Anemia, Aplastic↗
PubMed1982

Agar culture of bone marrow cells in acute myeloid leukemia and dysmyelopoietic syndromes. Reevaluation of its prognostic value.

The expression of results obtained in in vitro bone marrow cell culture as per ml of aspirated marrow instead of per 10(5) nucleated cells plated as studied in 41 patients with acute myeloid leukemia (AML) and 79 patients with a dysmyelopoietic syndrome. Four types of growth were found in AML patients: three with hypercellular marrow and either (a) microclusters, (b) isolated cells, or (c) macroclusters and a complete remission (CR) rate of 86%, 63%, and 14% respectively; (d) relative hypocellular marrow with a CR rate of 0%. This type of poorly responsive AML patient has recently been described [17]. Patients with dysmyelopoietic syndromes were subdivided into three groups according to th type of in vitro growth: subnormal, decreased, and leukemic. There was a good correlation between these in vitro groups and the outcome of patients: long survival, death from hemorrhagic and/or infectious complications, leukemic transformation (logrank test: chi 2 = 9.12, df = 2, p = 0.01).

Adult↗
PubMed1981

The histopathology and ultrastructure of liver disease in erythropoietic protoporphyria.

The light, polarization and electron microscopic changes in the liver of four cases of erythropoietic protoporphyria (EPP) are described. In one of these cases liver involvement resulted in death, whereas in the other three there were no symptoms of referable to hepatic disease. In two cases hepatic pigment was readily visible on light and polarization microscopy and corresponded to characteristic crystalline aggregates seen ultrastructurally. Pronounced micronodular cirrhosis and cholestasis were present in the fatal case, while in the other only mild periportal fibrosis and mononuclear inflammatory cell infiltrate occurred. The third and fourth cases showed no evidence of hepatic involvement on light microscopy. Electron microscopy, however, revealed characteristic crystal-containing vacuoles indicative of protoporphyrin deposition. The need for early detection of the hepatic lesion is emphasized.

Adolescent↗
PubMed1980

[In vitro culture of granulopoietic precursors in dysmyelopoiesis. Prognostic value (author's transl)].

96 patients with a dysmyelopoiesis have had an in vitro study of bone marrow CFUc at the time of diagnosis. A correlation between in vitro growth characteristics and the transformation in acute leukemia (AT) was searched. We found 5 types of in vitro growth: 3 with a "non-leukemic" pattern IA, IB, and IC, and 2 with a "leukemic" pattern II and III. In the type IA (normal growth) there is few death (16%), all without AT. In the types IB and ic with decreased in vitro growth, 40% of patients dead without an at and 25% after an AT. In the types II (with excess of clusters) and III (clusters without colonies) an AT was almost the case. The specificity, the positive and negative predictive values of in vitro growth type for the study of an AT outcome were the best of all that we obtained in the study of biological parameters in the dysmyelopoiesis. The study of bone marrow CFUc in dysmyelopoiesis can separate a group of patients with high risk of an AT outcome, which could be ameliorate with chimiotherapy.

Adult↗
PubMed1980

Aggregation of chlorophylls in monolayers. Part IV. The reorganisation of chlorophyll a in multilayer array.

The nature of the interaction between the chlorophyll a molecules in multilayer arrays obtained by the Langmuir-Blodgett technique is examined by electronic and infrared spectroscopies. Following the deposition of the multilayers, we observed a blue shift with time in the electronic spectra. This effect is monitored by infrared spectroscopy. The intensity of the coordinated ketone band is decreased while the intensity of the free ketone band is increased. These modifications are explained by the reorganization of the chlorophyll a molecules from an organized to a less organized one. The influence of H2O, D2O and SO2 vapors on the chlorophyll a multilayers give some informations on the role of water molecules in the aggregation of chlorophyll a in this ordered system. From these observations, a model is proposed for the multilayer arrangement implying two molecules of water per molecule of chlorophyll a.

Journal Article↗
PubMed1979

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