Search PubMed⌕ Search

Biomedical subjects

D Gaillard

Publications and source records attributed to D Gaillard.

At least 127 records · Page 7Linked to original sources
PubMed1984

Growth of preadipocyte cell lines and cell strains from rodents in serum-free hormone-supplemented medium.

Ob17 is a clonal cell line isolated from the epididymal fat pad of C57 BL/6J ob/ob mouse that differentiates into adiposelike cells in serum-supplemented medium. In serum-free medium, this cell line shows increased growth under the addition of insulin, transferrin, fibroblast growth factor (FGF), and a factor present in extract of rat submaxillary gland (SMGE). This medium is referred to as 4F. Epidermal growth factor or nerve growth factor cannot replace SMGE, whereas partially purified platelet extract can substitute for FGF but only partially for SMGE. 4F Medium is able to support the proliferation of cells from other established preadipocyte clonal lines, HGFu and 3T3-F442A, and also of preadipocyte cells isolated from the stromal-vascular fraction of rat and mouse adipose tissues. In each case 4F medium is insufficient to support the differentiation of these cells into adipocytes. Ob17 cells grown and maintained in serum-free hormone-supplemented medium retain the ability to convert to adiposelike cells after serum addition. This serum requirement for differentiation cannot be substituted by the addition of growth hormone or of other putative adipogenic factors, or both. The results are discussed with respect to the requirements for growth and differentiation of the 3T3-L1 and 1246 preadipocyte cell lines previously described.

Adipose Tissue↗
PubMed1984

[Cardiac stimulation in children. A multicenter study of 241 patients].

This study analyses the results of cardiac pacing in 241 children operated between 1965 and March 1982 in 9 french cardiac centres. The ages at primary implantation were: less than 5 years, 32.8 p. 100, 6 to 10 years, 33.6 p. 100 and 11 to 16.5 years, 33.6 p. 100. Atrioventricular block was congenital in 40.7 p. 100 of cases (98 children) and postoperative in 56.4 p. 100 (136 children) with 67 cases, after repair of isolated ventricular septal defect and 18 after repair of an endocardial cushion defect. The symptoms preceding pacing were syncope (67 cases), bradycardia (92 cases) and cardiac failure (33 cases). The electrocardiographic indications were third degree block in 66.8 p. 100 of cases. The pulse generators were usually implanted in the abdominal wall (71.8 p. 100). The power sources in service (August 1982) were lithium (74 p. 100) and isotopic batteries (26 p. 100). Myocardial electrodes were used in 93.4 p. 100 of cases; 82.2 p. 100 were made by Medtronic. Early problems included: infection (10 cases), displacement of endocavitary electrodes (3 cases), elevated thresholds (2 cases). The late problems encountered were due to fracture of the pacing electrodes (19 cases) and elevated thresholds (50 cases). Two hundred and seven children are alive and well. A total of 341 pulse generators were implanted, 90 p. 100 being VVI mode. In August 1982, 56.6 p. 100 were programmable or multiprogrammable. Despite the technical problems involved, the myocardial approach is still used with good results, especially in young children and babies. The endocavitary approach is an alternative after 5 years of age.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1982

[Colonic nerve network demonstrated by quinacrine].

Quinacrine can be used for fluorescence microscopy to visualize certain cell bodies and fibres in the myenteric plexus of the intestine, forming a network different from the cholinergic and adrenergic systems. In the Piebald mouse, animal model of congenital megacolon, the quinacrine positive plexus is thin in the distal narrowed segment and well developed in the large proximal zone. In Hirschsprung's disease, the quniacrine positive network has the same pathologic features but, contrary to the animal model, there is an increased number and size of extrinsic cholinergic and adrenergic fibres. So this mouse is an animal model of a pseudo-Hirschsprung's disease, which has not yet been reported in human pathology.

Adenosine Triphosphatases↗
PubMed1982

[Distribution of fibronectin in renal pathology (author's transl)].

The distribution of fibronectin (FN), a major glycoproteic component of extracellular matrix, has been detected in the human kidney by an indirect immunofluorescence technique using a rabbit anti-human FN serum on 6 embryonic renal tissues, 5 normal kidneys and 120 renal biopsies. In the metanephros, FN was seen within tubular basement membranes and in embryonic glomeruli. There was a progressive loss of tubular basement membrane staining during the differentiation of renal parenchyma. In the normal kidney and in kidneys with nephritis without endo- and/or extracapillary proliferation, FN was localized only in the mesangium and faintly in capillary walls of glomeruli. In glomerulonephritis with endo- and/or extracapillary proliferation, FN was increased around mesangial cells. FN was also bound to fibrin in epithelial crescents, fibrinoid necrosis and in thrombi of thrombotic microangiography. FN was increased in the mesangium of diabetic glomeruli without endocapillary proliferation. FN has not been found in amyloid deposits and in sclerosed glomeruli. We therefore conclude that FN is a good mesangial marker and is probably involved in the inflammatory process.

Diabetic Nephropathies↗
PubMed1981

Distribution of radioactivity following oral administration of carcinogenic 14CH3-labelled nitrosocarbaryl in the rat.

After a single intragastric administration of 14C-labelled carcinogenic nitrosocarbaryl, a nitrosated pesticide, the distribution of radioactivity was investigated in the blood and a number of organs in male rats. The animals received 0.25 mg/kg of labelled nitrosamine and were killed following administration at timed intervals between 0.5 h and 24 h. Our results show that the greatest amount of the 14CH3--group was associated with the forestomach, tumor-susceptible tissue; the level of radioactivity is noteworthy but less important in the glandular stomach. There are also sites of radioactivity accumulation mainly in the liver. Moreover, [14C]nitrosocarbaryl was revealed in the blood suggesting that nitrosamine itself rapidly (0.5 h) crosses the intestinal barrier and in a significant quantity (13%). These facts constitute a potential carcinogenic risk.

Animals↗
PubMed1981

[Supero-inferior ventricles and hearts with crossed circulation. Apropos of 2 cases. Review of the literature].

Two cases of supero-inferior heart are reported. Segmental analysis of the first case showed: situs solitus, atrioventricular (left sided loop) and ventriculoarterial discordance, resulting in a corrected transposition with the aorta in L malposition. The second malformation arose on a situs inversus, atrioventricular concordance (left sided loop) and double outlet right ventricle. The right ventricle was on the right and above the left ventricle giving an appearance of paradoxal discordance. The atrioventricular connections determined a plane of cleavage between right and left circulations in the supero-inferior ventricles and an appearance of crossed circulations in the second case. Hypoplasia of the inflow tract, of the right ventricular sinus is almost constant in this type of spatial orientation of the ventricles. The embryological hypoplasias are suggestive of an abnormality in the rotation of the cardiac tube in a frontal plane for the superimposed ventricles and abnormal rotation secondary to ventricular septation in the hearts with crossed circulations. The different classifications proposed in the literature are discussed with respect to these cases.

Child↗
PubMed1981

[Localized form of xanthogranulomatous pyelonephritis in a child. Case report].

The authors report the case of a 4 1/2-year-old boy with a localized xanthogranulomatous pyelonephritis. Review of the literature shows that this rare condition is often misdiagnosed before surgery, a preoperative diagnosis of Wilms' tumor being proposed instead of the exact one which is of benign prognosis. The pathogenesis is unknown.

Child, Preschool↗
PubMed1981

[Triploid embryonic molar pregnancy. Special aspects of one case (author's transl)].

Echotomography and a caryotype were carried out on the liquor and at an interval of one week between the two, two series of hormone levels were also carried out on the liquor and on the peripheral venous blood of a molar triploid pregnancy diagnosed clinically. These results have been compared with those that are known to occur in hydatidiform mole, and in other case histories that have been published of embryonic mole. The histological appearance of the fetal gonads resembles that found in Klinefelter's syndrome. The tests carried out on the HLA systems of the parents and of the fetus favours a maternal origin for the extra chromosomal complement.

Adult↗