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Biomedical subjects

D E Jones

Publications and source records attributed to D E Jones.

At least 127 records · Page 7Linked to original sources

Phenotypic profiles of broiler stocks fed two levels of methionine and lysine.

A phenotypic profile was compiled for eight commercial broiler stocks fed diets containing two levels of methionine and lysine. Stock by diet interactions were unimportant for most traits, although differences among stocks were somewhat magnified when they were fed the lower levels of methionine and lysine. Feed efficiency (body weight/feed consumption) to a fixed age was negatively associated with body weight, feed intake, frequency of leg disorders, and size of the abdominal fat pad. Its association with immunological and physiological traits varied with the environment. Apparent improvements in performance may be partially attributed to increases in growth rate, which have enabled broilers to reach market age at earlier ages.

Animals↗

Fertility and behavioral responses of roosters to diets differing in protein and calcium.

Diets differing in calcium (approximately 3.2 and .9%) and protein (15 and 12%) were fed to assess their effects on fertility, behavior, and physiological traits of White Leghorn males from lines selected for high (HA) and low (LA) antibody titers in response to the administration of sheep erythrocytes. Two trials, terminated when the males were 721 and 380 days of age, respectively, were conducted. In Trial 1, the HA males consumed significantly more feed than the LA males and those fed the low protein diets consumed more feed than those fed the high protein diets. No significant differences in feed intake were observed in Trial 2. Neither line, calcium level, nor protein level had a consistent effect on body weight in either trial. There were no significant first or second order interactions between main effects. Comparisons within ages and trials for percentage and duration of fertility revealed no significant differences between lines, protein level, or calcium level. Although the HA males produced significantly more antibodies to canine erythrocytes, dietary effects on antibody titers, heterophil: lymphocyte ratios, and plasma corticosterone were not significant. Thus, there was no indication that dietary treatments were inducing physiological adaptation. Behavioral adaptations, however, were observed; differences were significant for fear, territoriality, air pecking, and head shaking. It was concluded that behavioral responses symptomatic of nutrient deficiencies occur when no overt deficiency is present; this infers that an animal's well-being cannot be assessed strictly on the basis of behavioral consequences.

Animals↗

Elimination of multiple reactions of the Phadebact Streptococcus coagglutination test.

Strong multiple reactions often occur with the Phadebact Streptococcus test when the culture contains blood. These reactions interfere with the identification of the Lancefield groups of streptococci. Group B streptococci from the vagina of pregnant women are difficult to identify by slide coagglutination because of the frequent presence of blood on culture swabs. Elimination of these multiple reactions caused by blood would permit rapid identification of group B streptococci in pregnant women. Vaginal broth cultures were examined to determine the cause of multiple reactions with slide coagglutination and to eliminate them from the testing procedure. Of 245 maternal broth cultures, 135 (55%) yielded multiple reactions when tested by coagglutination. Such reactions were either eliminated or greatly diminished by heating the broth sample to 90 degrees C for 10 min. It was also found that globulins in the serum may be responsible for multiple reactions with blood. This heating protocol will permit vaginal broth cultures to be rapidly tested for group B streptococci by slide coagglutination.

Agglutination Tests↗

Rapid identification of pregnant women heavily colonized with group B streptococci.

Pregnant women admitted to Tampa General Hospital, Tampa, Fla., were cultured for group B streptococci (GBS). Culture swabs were placed into enriched, selective Todd-Hewitt medium and were quantitated for GBS. The broth cultures were tested by slide coagglutination before incubation and after 5 and 20 h of incubation. Fifty-four (27%) of the 201 maternity patients cultured were positive for GBS and were identified as such by slide coagglutination. A strong correlation was found between the magnitudes of colonization and the times required to identify the broth cultures as GBS positive. Cultures from mothers heavily colonized (mean concentrations of 3 X 10(4) GBS per culture swab or greater) were identified after 5 h or less of incubation. Mothers lightly colonized with GBS (mean concentrations of 2 X 10(2) GBS per culture swab) were identified only after their broth cultures had been incubated for 20 h.

Agglutination Tests↗

A light and electron microscopic study of the human ovarian ligament.

The structure of the human ovarian ligament was studied with light and electron microscopy, with emphasis on neural components. The ligament is primarily composed of smooth muscle arranged in an outer oblique and an inner longitudinal layer. The smooth muscle cells are connected by two types of intercellular junctional complexes, close contacts and intermediate junctions. The intercellular junctions may serve to coordinate smooth muscle activity and synchronize contractions of the ligament. Nerves are abundant and accompany the vascular supply. Specialized encapsulated neural structures previously reported were not observed. Acetylcholinesterase-positive nerves are more numerous than adrenergic nerves. Both types of nerves are associated with vascular smooth muscle. We conclude that these nerves either traverse the ligament or terminate in the ligament and are vasomotor.

Acetylcholinesterase↗

Dietary cellulose, wheat bran, and fish meal in relation to hepatic lipids, serum lipids, and lipid excretion in laying hens.

Isonitrogenous diets supplemented with 10% cellulose, 10% wheat bran, or 10% fish meal were fed to White Leghorn hens from 245 to 322 days of age. Neither body weights nor egg production of the hens fed these diets differed significantly from those of controls fed a corn-soybean meal basal diet. Supplemental fish meal and wheat bran did not significantly affect liver weight or liver lipids, although serum lipids of the hens fed the diet containing fish meal were significantly lower than those of the hens fed the basal diet. The hens fed the cellulose-supplemented diet, however, exhibited significantly decreased liver weights, serum lipids, and liver lipids, which were accompanied by a significant increase in excreta lipid. It is speculated that lipid excretion may be involved in some dietary induced alterations in the hepatic lipid concentration of laying chickens.

Animals↗

Burkitt's lymphoma: obstetric and gynecologic aspects.

A 17-year-old white patient with Burkitt's lymphoma occurring during pregnancy is reported. Although it is primarily a disease of children, Burkitt's lymphoma occurs in women of childbearing age. In nonendemic areas (including the United States) this disease most frequently presents as abdominal or pelvic masses, and may appear initially to be a primary gynecologic problem. Bilateral massive breast enlargement due to tumor metastasis is always seen if the disease occurs during pregnancy. Although 5 previously reported pregnant patients and the present patient died, survival may be enhanced by prompt diagnosis and combination chemotherapy and radiation therapy. Obstetric and gynecologic aspects of Burkitt's lymphoma are discussed.

Adolescent↗