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Biomedical subjects

D Dubois

Publications and source records attributed to D Dubois.

At least 73 records · Page 4Linked to original sources
PubMed1987

Growth of animal cells on microbeads. II. Estimation of biomass in situ and observation of cytopathic effects after infection of McCoy cells with Chlamydia trachomatis.

The Channelyzer C256 together with the ZB Coulter Counter have been used to study in situ the growth kinetics of McCoy cells attached on Cytodex 3 microbeads. The number of cells per microbead varied linearly with the average size of the covered microbeads, showing that electronic particle sizing can be used to measure biomass or cell numbers. As the cells became confluent, an increase in cell volume with little accompanying increase in cell number was detected. The technique also enables the experimenter to follow in situ the development of cytopathic effects resulting from the infection by pathogens such as Chlamydia trachomatis serotype L1.

Animals↗
PubMed1986

[Lyell's syndrome induced by Vectren].

While treated with isoxicam, a middle-aged lady developed first diffuse maculopapules and afterwards a syndrome of Lyell; similar observations of bimodal toxicodermatitis have been reported in the literature.

Arthritis↗
PubMed1986

[Familial predisposition to multiple sclerosis].

Seven families were studied to evaluate the influence of genetic factors in MS and its transmissibility. The disease was associated with some B7 and/or DR2 alleles in relation to the susceptibility gene in all but one case. This association was always transmissible conjointly. The carrier haplotype conditions disease manifestations and its prognosis, favorable in this series, but it is not sufficient to promote the onset of the disease. This requires a triggering factor with sometimes disorders affecting 3 generations as demonstrated by the analysis of one family. Therefore there is not a genic heredity but a predisposition heredity. Possible associations of MS with other morbid states such as insulin-dependent diabetes must be considered when counselling families.

Adolescent↗
PubMed1985

A fluorometric assay for the biotin-avidin interaction based on displacement of the fluorescent probe 2-anilinonaphthalene-6-sulfonic acid.

Avidin and biotin can be sensitively and accurately quantitated using the fluorescent probe 2-anilinonaphthalene-6-sulfonic acid (2,6-ANS). In the presence of avidin, the fluorescence of 2,6-ANS is blue shifted with a large increase in quantum yield. Biotin binding causes complete displacement of the bound fluorophore with concomitant quenching of the fluorescence. The fluorometric monitoring of the displacement of 2,6-ANS can be used as a facile method of measuring the biotin-avidin interaction. 2,6-ANS displacement gives the same stoichiometry as the method using 4'-hydroxyazobenzene-2-carboxylic acid. Our initial studies of an affinity-purified avidin revealed that, of the four binding sites on the avidin tetramer, a mean of three remain available for biotin (or dye) binding; this finding highlights a caveat concerning the use of affinity-purified oligomeric-binding proteins with multiple sites. As compared with previous fluorescence methods, the use of 2,6-ANS gives high sensitivity without the necessity of preparing and purifying a covalent avidin conjugate. In addition, the present method;:is potentially more sensitive than those based on optical absorbance; uses a probe that has increased stability and a larger Stokes shift compared with fluorescein; is not subject to protein interference; and gives accurate results over a wide range of 2,6-ANS and avidin concentrations.

Anilino Naphthalenesulfonates↗
PubMed1985

[Gonorrhea and pustules of the hands].

Two similar cases of disseminated infection by Neisseria gonorrhoeae were observed at the same time. The patients presented with fever, arthralgia, tenosynovitis, cutaneous pustules of the extremities. In one case, N. gonorrhoeae has been recovered from vaginal discharge; in the other, bacteriological investigations failed. From a review of the literature, it appears that: (1) acral vesiculo-pustular lesions and tenosynovitis represent the most suggestive signs of the disease; (2) it is obviously important to repeat the bacteriological investigations; (3) biological data, included serology, are useless. However, immunofluorescence techniques might detect altered germs in the skin.

Adult↗
PubMed1984

[Association of familial hyperparathyroidism and Cushing's disease].

Five members of one family had been operated on for primary hyperparathyroidism. One of them also had Cushing's disease (i.e. pituitary tumor). An association between familial hyperparathyroidism and Cushing's disease is quite unusual. Such a combination of rare diseases is not fortuitous though; it probably is but a special type of multiple endocrine neoplasia.

Adult↗
PubMed1983

[New method of weaning from respiratory assistance. Ventilation based on carbon dioxide].

The authors describe a new device for weaning from mechanical ventilation, based on continuous measurement of end-expiratory concentration of CO 2 (FCO 2 ET). The spontaneous or controlled mode of ventilation is automatically determined by the level of FCO 2 ET in relation to preselected maximum and minimum thresholds. The authors call this device "CO 2MV".

Breath Tests↗
PubMed1983

Angiotensin-converting enzyme in human adult respiratory distress syndrome.

Angiotensin-converting enzyme (ACE) levels, complement activation, and intravascular coagulation were studied in 36 patients with adult respiratory distress syndrome (ARDS) (17 aseptic, 19 septic), in order to investigate the possible interrelations among ACE, immunologic data, and hematologic findings. The severity of respiratory impairment was assessed with measurements of mechanical and gas exchange functional qualities of the lung. Serial measurements of ACE could be done in 14 patients during an eight-day period. During the first 24 hours, ACE levels were always normal (38 percent) or decreased (62 percent). No difference could be found between patients with septic and aseptic ARDS. Complement activation occurred in 78 percent (28/36) and used, in most cases, the classic pathway with presence of circulating immune complexes. Criteria for intravascular coagulation were present in 58 percent (21/36). No relation between coagulation, complement, and ACE could be found except for the patients with a greater respiratory impairment, who had complement activation, intravascular coagulation, and significantly lower ACE levels. In all patients together, ACE levels had no diagnostic value for aseptic cause of ARDS and a poor prognostic value. Only intravascular coagulation was linked with a higher significant mortality and a greater functional impairment. Serial measurements showed a diphasic evolution of ACE levels, with a maximum decrease between the 72nd and 96th hours and a further normalization (seventh day). The persistence of low levels seemed to be associated with evolutive sepsis or secondary aggravation and fibrosis.

Adolescent↗
PubMed1983

Beta blocker therapy in 125 cases of hypertension during pregnancy.

Over a period of 4 years, a team of obstetricians and nephrologists have used beta-blockers in the treatment of hypertension in high risk pregnancies. One hundred and twenty one patients (125 pregnancies) were treated with this new therapeutic approach: Acebutolol (56 cases), Pindolol (38 cases) and Atenolol (31 cases) were used. In our group of patients, 56% (70/121) had a previous record of hypertension. Treatment was started when diastolic pressure reached 90 mmHg. The mothers showed excellent tolerance and in 95% of cases blood pressure was controlled in a satisfactory manner. Three groups of new-born infants were defined. In 20 infants, the weight was less than 2.5, in 15 infants between 2.5 and 2.8 kg, and in 90 infants more than 2.8 kg. There was no evidence of low Apgar scores, bradycardia or hypotension in the infants. The importance of team management of the patient is emphasised.

Abnormalities, Drug-Induced↗