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Biomedical subjects

D Cole

Publications and source records attributed to D Cole.

At least 109 records · Page 6Linked to original sources
PubMed1984

Subcellular Localizations of Two Dolichos biflorus Lectins.

The subcellular localizations of the Dolichos biflorus seed lectin and the structurally related lectin (cross-reactive material [CRM]) from the stems and leaves of this plant were determined by immunofluorescence, immunocytochemistry, and cell fractionation procedures. Subcellular fractionation of the cotyledons using a nonaqueous procedure to minimize disruption of the protein bodies showed that the majority of the seed lectin was associated with the protein body fraction and some lectin was also present in the starch granules. Immunofluorescence and immunocytochemistry at the light microscopic level showed that the seed lectin was mainly localized at the peripheries of these organelles. Lectin was also found in the cytoplasm of the cells, although the amount appeared to be dependent upon the degree of protein body disruption.Immunofluorescence and immunocytochemistry studies of the stem and leaf lectin (CRM) indicated that a significant portion of this lectin may be associated with the cell walls, although lectin was also seen in the cytoplasm of plasmolyzed cells. Extraction and cell fractionation studies showed that a large portion of the CRM is readily solubilized and most of the remainder is pelleted at 1000g. The CRM can be extracted from these pellets by treatment with cellulase and pectinase; other reagents such as NaCl, detergents, and EDTA could also release significant amounts of CRM. These studies suggest that the CRM is noncovalently bound to the cell walls. A comparison of the distribution of exogenously supplied [(125)I]CRM with the endogenous CRM during extraction and cell fractionation indicates that soluble CRM is not adsorbed to the 1000g pellet during fractionation.The different subcellular distributions of these two structurally related lectins suggest that different tissues of the same plant may utilize lectins for different functions.

Journal Article↗
PubMed1984

Evidence for a reappraisal of the psychophysical selective adaptation paradigm.

The human psychophysical adaptation literature infers the existence of channels in the auditory system sensitive to frequency modulation (FM) from selective increases in FM detection thresholds following adaptation with FM stimuli. Using this psychophysical paradigm to characterize the attributes of feature-sensitive channels requires knowledge of the phenomenon's stability over repeated testing. In this study FM detection thresholds were measured in human subjects with continued testing over numerous sessions. During adapting intervals within the sessions either FM upsweeps or silence was presented. Exposure to FM upsweeps initially resulted in an increase in FM detection thresholds by a factor of 2 to 3 relative to those measured following silence. These initial threshold elevations decreased markedly with repeated testing (more than five 30-min experimental sessions). Final threshold differences between adapted and nonadapted conditions approached zero. In one subject, such asymptotic threshold values were regained in a single session, after a 4-month hiatus. The findings suggest multiple determinants of the selective adaptation function, as well as a reevaluation of inferred mechanisms.

Adaptation, Physiological↗
PubMed1983

Heterogeneity of acute lymphocytic leukemia cell surface markers as detected by monoclonal antibodies.

Monoclonal antibodies 3A1, 4F2, 5E9, OKT3, OKT6, and OKT8 were tested by flow microfluorometry for reactivity with cells from patients with T-cell or null cell acute lymphocytic leukemia (ALL). The 3A1 antibody reacted with a greater percentage of cells from 1 group of patients that could be classified as T-cell leukemia patients as compared to its reaction in the group classifiable as null cell leukemia patients. Reactivity patterns of other monoclonal antibodies with lymphocytes from patients with ALL did not clearly define groups of ALL patients. All cells from individual leukemia patients reacted differently with the seven monoclonal reagents. On the basis of patterns of reactivity, T-cell leukemias could not be classified as malignant clones of cells from the previously described stages of thymus cell maturation. The results demonstrated that the cells undergoing malignant change express a variety of combinations of antigens not found on normal lymphocytes.

Antibodies, Monoclonal↗
PubMed1982

Adolescents who take overdoses: their characteristics, problems and contacts with helping agencies.

In a consecutive sample of 50 adolescents aged 13-18 admitted to hospital after taking overdoses, 90 per cent were girls. There were other clear differences between this sample and adolescents in general. Twenty-four per cent had visited their general practitioners in the previous week, and 50 per cent during the previous month. The most common difficulties preceding the overdoses were problems with parents, boys or girlfriends, and with school or work, including unemployment. A substantial proportion of the subjects had recent recurrent physical ill health. In the majority of cases the problems appeared to be transient so that one month later two-thirds of the adolescents had shown considerable general improvement. However, 14 per cent were referred to hospital for further self-poisoning or self-injury in the following year. The recent increase in the incidence of self-poisoning among adolescents suggests that more attention must be paid to this group in terms of both primary and secondary prevention.

Adolescent↗
PubMed1982

Classification of adolescents who take overdoses.

A simple method of classifying adolescent self-poisoners into three sub-groups on the basis of the duration of their problems and the presence or absence of behavioural disturbance is described. The three groups differed markedly in terms of factors concerning their families, especially their relationships with their parents, and in their medical and psychiatric histories, the nature of their overdoses and the problems they were facing at the time. The treatment offered to members of the three groups also differed. The method of classification may offer a useful means of distinguishing between subjects in terms of outcome, including subsequent adjustment and repeat attempts.

Adolescent↗
PubMed1982

Motivational aspects of deliberate self-poisoning in adolescents.

A systematic study of 50 adolescent self-poisoners aged from 13 to 18 demonstrated considerable discrepancies between the reasons chosen by the subjects to explain the overdoses and those chosen by clinical assessors. Most adolescents indicated that they had been feeling lonely or unwanted, or angry with someone, and had taken the overdose to alleviate or demonstrate this distress. A third said they had wanted to die. In contrast, clinical assessors tended to attribute the overdose to punitive or manipulative reasons and suggested that only seven out of the 50 had wished to die. The adolescents rarely indicated that they had taken the overdose to get help; this may explain the resistance that may be shown to psychiatric intervention, that casts doubt on the possible effectiveness of preventive agencies. Modification of attitudes to both self-poisoning and early help-seeking may be a more effective means of prevention.

Adolescent↗
PubMed1976

Combined simultaneous radiotherapy and chemotherapy for treatment of unresectable and/or metastatic cancer.

The present program was started in 1971 and is being conducted as a probing study. We believe that the only conclusion that can be drawn thus far is that, by utilizing the conventional cobalt therapy and chemotherapy together, there has been no increase in morbidity or mortality. The patients appear to have tolerated this therapy well on an out-patient basis with hospitalization only being needed occasionally. By the time the radiotherapist and/or chemotherapist sees the patient, the cancer is usually systemic. Therefore, a combined approach should be seriously considered since we have found that the patients tolerate this program well. The authors also feel that there has been an improvement, thus far, in terms of completion and duration of response. Further studies are currently being carried out in order to arrive at a more satisfactory statistical conclusion.

Adult↗
PubMed1975

A state-wide survey of index pediatric surgical conditions.

A statewide survey of pediatric surgery was sponsored by the Connecticut Society of American Board Surgeons to determine the general surgeons attitudes about performing routine and complex pediatric surgery, the absolute volume of index pediatric surgical conditions in the state, and the proportion treated by pediatric and nonpediatric surgeons. The incidence of index conditions studied was very similar to those previously reported. Approximately one half of all index cases were treated by nonpediatric surgeons, indicating that the number of pediatric surgeons could be increased, and possibly doubled, if all index procedures were performed by pediatric surgeons. Two-thirds of responding surgeons recognized the need for a regional neonatal surgical center. Regional or statewise surveys of index surgical conditions are recommended to determine regional manpower needs.

Adolescent↗
PubMed1974

Douglas Robb.

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General Surgery↗
PubMed1972

Homograft valves.

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Anti-Bacterial Agents↗