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Biomedical subjects

D C Moore

Publications and source records attributed to D C Moore.

At least 55 records · Page 3Linked to original sources
PubMed1991

Vitamin D analogs inhibit erythroid differentiation and induce monocytic differentiation of leukemic cells with the same relative potency.

Myeloid leukemia cells of human and murine origin can be induced to differentiate into more mature forms which lose their neoplastic properties. The hormonal form of vitamin D is a powerful inducer of monocytic differentiation, but its therapeutic use is limited by hypercalcemia. It was recently reported that a novel derivative of vitamin D, 1,25-dihydroxy-16-ene-23-yne-vitamin D3, is an exceptionally potent inducer of monocytic differentiation, and prolongs survival of mice bearing leukemia cells. We now show that this compound is also a most potent inhibitor of erythrodifferentiation. This finding has important implications for the control of hematopoiesis.

Calcitriol↗
PubMed1991

Autogeneic bone marrow and porous biphasic calcium phosphate ceramic for segmental bone defects in the canine ulna.

The purpose of this study was to evaluate a porous biphasic hydroxyapatite-calcium phosphate ceramic as a modifier and extender of an autogeneic marrow graft for filling a 2.5-cm segmental bony defect. Twenty adult mongrel dogs were surgically treated to create diaphyseal defects in the left ulnae. The defects were (1) filled with autogeneic bone marrow mixed with granular hydroxyapatite-tricalcium phosphate ceramic (granular ceramic); (2) grafted with a solid block of ceramic soaked in autogeneic bone marrow (block ceramic); (3) received no graft (no implant); or (4) were grafted with autogeneic bone marrow alone (bone marrow). All animals were followed clinically and roentgenographically for 24 weeks and then killed. Repair of diaphyseal defects with the block ceramic led to three solid unions and three fibrous unions; with the granular ceramic implants and marrow, the defects of five dogs formed solid unions, and one progressed to a fibrous union. Defects in all five dogs grafted with autogeneic bone marrow united. The three dogs with no implant formed nonunions. Histology showed normal marrow and only a light immune reaction. Complete bridging of the defect in the dogs treated with the granular ceramic occurred significantly earlier than bridging in the dogs grafted with bone marrow alone. Histomorphometry, performed on the block ceramic implants indicated active resorption of ceramic. Clinically, addition of ceramic to a marrow graft improved the handling characteristics of the graft material and accelerated healing according to roentgenographic evaluation.

Animals↗
PubMed1990

Body image and eating behavior in adolescent boys.

A questionnaire about attitudes toward body weight and shape, and eating and weight control practices, was administered to 895 adolescent boys. Overall, 42% were dissatisfied with their weight and 33% with their body shape. Sixty-eight percent of the dissatisfied normal weight boys thought they were underweight and 32% thought they were overweight. Subjects dissatisfied with their body shape were most likely to desire an increased chest and arm size and decreased abdomen size. Binge eating (24%) and fasting (12%) were the most prevalent eating practices and were more common in boys dissatisfied with weight or shape. There was no difference in prevalence of eating practices between boys wishing to gain and boys wishing to lose weight. Although many boys were concerned about body weight and shape, they wished to increase weight and muscularity, rather than lose weight. Binge eating was common, but was not related to the binge-purge cycle described in girls, because losing weight is not a major concern, and other weight control practices were not more common among boys who wanted to lose weight vs gain.

Adolescent↗
PubMed1990

Predictors of improvement in tardive dyskinesia following discontinuation of neuroleptic medication.

Forty-nine chronic psychiatric out-patients (ten were schizophrenic) with tardive dyskinesia (TD) were examined monthly for a mean of 40 weeks (range 1-59 months) after discontinuation of neuroleptic medication. Complete and persistent reversibility of TD was rare (2%), but many patients showed noticeable improvement in movements within the first year of discontinuation, which was sometimes interrupted by psychological relapse. Using three separate outcome measures and appropriate model-fitting techniques for each, we identified several predictors of improvement in TD, including an affective or schizoaffective psychiatric diagnosis, chronic (over 20 years) psychiatric illness, being employed, younger age, and increased neuroleptic dose before discontinuation. Consistent findings emerging from these analyses suggest that the type and history of psychiatric illness affect the course of TD.

Adult↗
PubMed1989

Morphological and physiological effects of beta-hydroxybutyrate on rat embryos grown in vitro at different stages.

Diabetic women are more likely to give birth to infants with congenital malformations than are nondiabetic women. Rodent embryos have been used as a model for the study of abnormal fetal development associated with maternal diabetes, and some of the metabolic factors which are altered in diabetes, such as raised glucose and ketones, have been shown to cause abnormal development of rodent embryos in vitro. The present work explores further the teratogenicity of beta-hydroxybutyrate to rat embryos. To determine the sensitivities of rat embryos at different stages of their development, rat embryos at 9.5 days of gestation have been cultured in vitro for 24 or 48 h, with or without 4 x 10(-2) M beta-hydroxybutyrate for all or part of the culture period. The embryos have been examined by scanning electron microscopy, and a detailed morphometric analysis of one tissue, the neuroepithelium, has been undertaken. The results confirm that beta-hydroxybutyrate causes abnormal development of rat embryos. The results of experiments in which embryos were exposed to beta-hydroxybutyrate for only part of a 48 h culture show that embryos exposed to beta-hydroxybutyrate for a complete 48 h culture are more severely affected than embryos exposed to beta-hydroxybutyrate for only part of the culture and that embryos are more vulnerable to beta-hydroxybutyrate during the first half of a 48 h culture (equivalent to 9.5 to 10.5 days of gestation) than during the second half of a 48 h culture (10.5 to 11.5 days of gestation). The results of experiments in which embryos were cultured with beta-hydroxybutyrate from 9.5 days of gestation for 24 h (equivalent to 9.5 to 10.5 days of gestation) showed that some effects of beta-hydroxybutyrate are already apparent after 24 hours in culture. Many of the abnormalities produced by beta-hydroxybutyrate can be classified as embryonic retardations rather than malformations--that is, embryos show features characteristic of normal, but younger, embryos. Embryos exposed to beta-hydroxybutyrate for the complete 48 h culture period consume less glucose and produce less lactate than control embryos on a per embryo basis, but not on a per microgram protein basis, suggesting that the reduced metabolism is an effect of beta-hydroxybutyrate-induced developmental delay rather than a cause of it.(ABSTRACT TRUNCATED AT 400 WORDS)

3-Hydroxybutyric Acid↗
PubMed1989

Serum cortisol responses in febrile children.

Adrenocortical stress response in children with a variety of febrile illnesses was prospectively evaluated in 76 patients presenting to a general pediatric clinic with temperature greater than 101 degrees F (38.3 degrees C). Serum cortisol concentrations at presentation and again after recovery from infection were determined. Overall mean magnitude change in cortisol concentrations was 3.6-fold. Cortisol response was unrelated to the height of temperature but significant differences depending on clinical diagnosis were identified. The largest response (5-fold) was observed in patients with pneumonia, bacterial meningitis and fever of undetermined etiology. Current recommendations to double or triple replacement hydrocortisone dosage during times of increased stress in children with adrenal insufficiency are adequate only for simple febrile illnesses such as upper respiratory infection and streptococcal pharyngitis but could be subtherapeutic for infections such as pneumonia, meningitis and fever of undetermined origin, which imply a greater systemic involvement. It is possible, but untested, that a 4- to 5-fold increase in dosage would be more appropriate in those conditions.

Adrenal Cortex↗
PubMed1989

Intralenticular abscess.

Intralenticular abscess is an uncommon complication of surgery or trauma. A case of intralenticular abscess following penetrating trauma is presented. In this case, the offending organism was identified as Propionibacterium acnes. This organism is considered to be a cause of slowly progressing endophthalmitis and has been implicated as a cause of the so-called toxic lens syndrome.

Abscess↗
PubMed1988

Body image and eating behavior in adolescent girls.

To determine attitudes toward body weight and shape and eating and weight control practices among adolescent girls, an anonymous questionnaire was administered to 854 adolescent girls and young women aged 12 through 23 years who were seen in a military adolescent outpatient clinic. Overall, 67% were dissatisfied with their weight, and 54% were dissatisfied with their body shape. Dissatisfaction with weight and shape varied positively with increasing body weight but not with increasing age. Binge eating had occurred in 30.4%, and weight control behavior, such as dieting, fasting, vomiting, and stimulant, laxative, and diuretic use, had occurred in 38.2%, 30.7%, 8.5%, 9.5%, 3.3%, and 6.2%, respectively, varying positively with increasing weight. Thirty-six percent of those adolescent girls who saw themselves as overweight desired an inappropriate weight loss, and 61% of these, who desired an excessive loss, exhibited an increased prevalence of weight control behaviors and were less likely to believe that they had an eating problem. Dissatisfaction with body weight and shape, and eating behaviors, such as dieting, binge eating, fasting, and vomiting, are common in adolescent girls, many of whom are attempting weight control without an accurate perception of what is normal.

Adolescent↗
PubMed1988

Prenatal sonographic diagnosis of Pena-Shokeir syndrome type I, or fetal akinesia deformation sequence.

We report on a familial case of Pena-Shokeir syndrome type I (fetal akinesia deformation sequence) born to healthy parents. The antenatal ultrasound diagnosis was based on hydramnios, restricted limb movements, decreased fetal chest movements, small chest, arthrogryposis, clubfoot, fixed extension of knees, fixed flexion of elbows, camptodactyly, kyphosis of thoracic spine, cryptorchidism, and small muscle bulk. Thymic hyperplasia was noted at autopsy.

Abnormalities, Multiple↗
PubMed1988

Classical versus low-segment transverse incision for preterm caesarean section: maternal complications and outcome of subsequent pregnancies.

In a retrospective, controlled, follow-up study of 326 women who had a primary preterm caesarean section, the risks of postoperative maternal morbidity and uterine rupture or dehiscence in subsequent pregnancies were investigated in relation to the mode of incision (classical compared with low-segment transverse incision). The classical incision was associated with a higher frequency of postpartum fever in the immediate postoperative period (16% compared with 6%, P less than 0.01). Of the 326 patients reviewed 286 (88%) were contacted for information about subsequent pregnancies. Information was obtained for 70 pregnancies subsequent to a classical caesarean section, and 71 pregnancies subsequent to a low-segment transverse caesarean section, which had continued for more than 20 weeks gestation. Of the pregnancies after the classical operation 13% had abnormal scars compared with none of those after the low-segment transverse operation (P = 0.0014). The frequency of scar dehiscence was 6% after a classical scar compared with none after a low-segment transverse scar (P = 0.0581).

Adult↗
PubMed1988

Perturbation of in vitro development of rodent embryos by calcium antagonist Quin-2.

Tissue shaping during embryogenesis is driven, at least in part, by changes in the shapes of the cells of the tissue undergoing morphogenesis. These cell shape changes are effected by microfilaments, and appear, as do microfilament-mediated events in non-embryonic cells, to be calcium-dependent. To explore the possible role of calcium in morphogenesis in mammalian embryos, the effects of Quin-2, a calcium-binding agent which can be loaded into cells as the acetomethyl tetraester (Quin-2-AM), on rat embryos cultured in vitro were determined. Quin-2-AM at 5 x 10(-5) M concentration delays the development of, and subsequently causes the abnormal development of, rat embryos cultured in vitro. In addition, Quin-2-AM inhibits the normal re-elevation of the cephalic neural folds which have been caused to collapse by exposure to medium containing a low concentration of calcium. These results suggest that morphogenetic movements, such as neurulation, depend upon an intracellular relocation of calcium, which can be perturbed by the calcium-binding capacity of Quin-2-AM.

Aminoquinolines↗
PubMed1987

The effects of mannose on rat embryos grown in vitro.

Rat embryos have been cultured in vitro from 9.5 days of gestation for different times in serum containing mannose, and the embryos have been observed by scanning electron microscopy. Embryos cultured in 3 mg/ml (1.7 X 10(-2) M) or 6 mg/ml (3.3 X 10(-2) M) mannose for 48 h showed inhibition of the expansion of the yolk sac and were smaller than the control embryos. Mannose-treated embryos also showed delayed development according to morphological criteria, and a range of abnormalities including abnormalities of the neural tube. Embryos cultured in 6 mg/ml mannose for 24 h also showed significant inhibition of yolk-sac expansion and were smaller and less advanced than the control embryos. Abnormalities were seen, including a delay in the closure of the neural folds. Abnormalities were also observed in embryos cultured in mannose for 10 h; embryos at the neural groove stage showed irregularities in the neural groove. Mannose did not inhibit the re-elevation of neural folds which had been caused to collapse by exposure to medium containing low calcium. These results are compatible with the idea that mannose retards development and thereby perturbs the morphogenesis of the neural tube.

Animals↗
PubMed1987

Sequence conservation in the 3'-untranslated regions of neurone-specific enolase, lymphokine and protooncogene mRNAs.

The C-terminal protein-coding and the entire 3'-untranslated regions of a cDNA corresponding to human neurone-specific enolase mRNA have been sequenced. The 3'-untranslated region is 892 bases long and shows a high degree of homology with the 3'-untranslated region of rat neurone-specific enolase mRNA. This sequence conservation is not seen in non-neuronal enolase mRNAs. Features of the conserved sequence include an A-rich region approx. 250 bases from the stop codon at a point corresponding to the polyadenylation signal site in non-neuronal enolase mRNA, and a repeating ATTT sequence. This unusual motif in eukaryotic mRNAs has previously been reported in the 3'-untranslated regions of lymphokine and protooncogene mRNAs.

Amino Acid Sequence↗