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Biomedical subjects

D C Davis

Publications and source records attributed to D C Davis.

At least 55 records · Page 3Linked to original sources
PubMed1971

The isolation of lipoproteins from human plasma by ultracentrifugation in zonal rotors.

The major classes of lipoproteins were isolated from human plasma by ultracentrifugation in continuous density gradients using the Ti-14 and Ti-15 zonal rotors. Chylomicrons + VLDL, LDL, and HDL were separated from each other and from the more dense residual proteins (albumin fraction) of plasma by rate-zonal flotation in NaBr gradients in the density range 1.0-1.4. The chylomicron-VLDL fraction was subfractionated into constituent chylomicrons and VLDL by zonal ultracentrifugation in NaBr gradients in the density range 1.0-1.1. Plasma lipoproteins were analyzed for composition of lipids and content of protein, for electrophoretic mobility on paper, and for antigenic determinants by immunoelectrophoresis and immunodiffusion. Flotation constants (S(f)) of the LDL and HDL were calculated from measurements made in the analytical ultracentrifuge. Lipoproteins isolated from plasma by zonal ultracentrifugation were identical by these criteria to lipoproteins isolated by the usual procedure of sequential ultracentrifugation in solvents of increasing density. The procedure of zonal ultracentrifugation is rapid, quantitative, and less laborious than sequential techniques. Lipoproteins isolated by zonal ultracentrifugation are relatively uncontaminated by other proteins and extensive washing is therefore unnecessary. Zonal ultracentrifugation is more than a preparative method for the plasma lipoproteins; it is also an analytical procedure in that a record is obtained of the distribution and quantity of the lipoprotein within the continuous density gradient.

Blood Proteins↗
PubMedDate not supplied

The effect of cocaine abuse on plasma levels of sulfated dopamine and salsolinol in alcoholics.

This study investigated the effect of cocaine abuse on peripheral dopamine and its tetrahydroisoquinoline metabolite salsolinol in chronic alcoholics. Specifically, the concentration of dopamine sulfate and salsolinol sulfate was measured in plasma samples obtained from the blood of a group of alcoholics (n = 40) and alcoholics with cocaine dependence (n = 55). The concentrations of sulfoconjugated dopamine and salsolinol were measured by a radioenzymatic technique. The results of this study showed that chronic alcoholics (627 +/- 195 pg/ml) and alcoholics with cocaine addiction (409 +/- 76 pg/ml) had significantly (p < 0.05) elevated levels of salsolinol sulfate (mean +/- SEM) in their plasma as compared to controls (99.5 +/- 7.5 pg/ml). However, alcoholics with cocaine dependence produced significantly (p < 0.01) higher concentration of dopamine sulfate in their plasma (7520 +/- 1299 pg/ml) as compared to chronic alcoholics (3896 +/- 438 pg/ml) and controls (2124 +/- 104 pg/ml). Differences in plasma dopamine sulfate among alcoholics with cocaine dependence vs. alcoholics without cocaine dependence may be interpreted as a reflection of increased extracellular dopamine metabolism associated with chronic cocaine exposure.

Adolescent↗
PubMedDate not supplied

No heroic measures.

Explore the source record for details and available documents.

Euthanasia↗
PubMedDate not supplied

Challenges and rewards of educating a profoundly deaf student.

The faculty and administrators of a college of nursing in a public university learned that awareness of the Americans With Disabilities Act of 1990 is very different from complying with its mandates in the education of a profoundly deaf nursing student. The process began with many challenges and ended with rewards beyond the expectations of all involved. Faculty, who initially resisted the concept of educating a "deaf nurse," developed creative teaching strategies and ultimately gained a new perspective regarding who has the potential to become a good nurse. In the final analysis, everyone agreed that this exceptional young woman was not only our student, but was also our teacher.

Communication↗
PubMedDate not supplied

A conceptual framework for infertility.

Infertility is a life crisis situation for the couple who desires children. A conceptual framework for viewing the life crisis effects of infertility is presented. King's conceptual framework for nursing serves as the organizational scheme. The personal system, interpersonal system, and social system effects of infertility are described.

Counseling↗
PubMedDate not supplied

Coping strategies of infertile women.

This study was initiated to explore the coping patterns of infertile women. The researchers interviewed 30 women and evaluated data using content analysis. Six ways of coping with infertility were identified: (1) increasing the space or distancing oneself from reminders of infertility, (2) instituting measures for regaining control, (3) acting to increase self-esteem by being the best, (4) looking for hidden meaning in infertility, (5) giving in to feelings, and (6) sharing the burden with others.

Adaptation, Psychological↗