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Biomedical subjects

D Bishop

Publications and source records attributed to D Bishop.

At least 55 records · Page 3Linked to original sources
PubMed1998

The duration of predicting trials influences time to fatigue at critical power.

The present study compared times to fatigue at CP which had been calculated from relatively long duration predicting trials. With eight recreationally active males (mean age +/- SE = 18.6 +/- 2.1 years) having first cycled to fatigue on five occasions at different fixed work rates, CP was calculated in three ways: 1. using data from the three lowest exercise intensities (CPl); 2. using data from all five exercise intensities (CPa); and 3. using data from the highest three exercise intensities (CPh). Although CP was calculated using a linear and a three-parameter non-linear model, there were insufficient suitable data to complete the latter analysis. After three days and over an eight day period, the subjects cycled for up to 60 minutes at each of the three CPs calculated using the linear model. Analysis revealed that despite high linearity with the five-point work-time regression (average r2=0.996), CPl, CPa and CPh significantly differed to each other (268 +/- 17.5W; 285 +/- 12.1W; 321 +/- 8.8W respectively; p<0.05). Significant differences were also found between times to fatigue at CPl, CPa and CPh (42.9 +/- 3.9, 39.9 +/- 4.6 and 34.4 +/- 2.7 minutes respectively; p<0.05). The data show that when CP is calculated using a linear work-time regression, time to fatigue at CP is significantly influenced by the duration of predicting trials. Moreover, exercise at CP could only be maintained for 43 minutes despite CP being calculated from predicting trials averaging between 10 and 25 minutes.

Adolescent↗
PubMed1998

The effect of stage duration on the calculation of peak VO2 during cycle ergometry.

This study investigated the influence of stage duration on the calculation of peak oxygen consumption (peak VO2 to determine whether both the lactate threshold (LT) and peak VO2 could be measured during the same test without compromising the peak VO2 value obtained. Eight moderately-active females (mean age +/- SD = 19.6 +/- 2.5 years) performed three peak VO2 tests on an electrically-braked cycle ergometer. Power output was increased every minute for the short peak VO2 test (S) and every three minutes for the long peak VO2 tests (L). Testing took place over two weeks with all tests separated by at least 48 hours. The first peak VO2 test was a long test (L1) and served as familiarisation. The subjects then performed a short (S) and a long (L2) peak VO2 test in random, counterbalanced order. For each subject, all three tests were performed at the same time of day in controlled environmental conditions. There was no significant difference between the two exercise protocols for peak VO2 when expressed in ml x kg(-1) x min(-1) (F[1,7]=3.47, P=0.105) or in L x min(-1) (F[1,7]=3.39. P=0.108). However, the maximum heart rate (HRmax) achieved in S was significantly less than the HRmax achieved in L2 (F[1,7]=33.4, P<0.001). The power output at exhaustion (Wpeak) was significantly greater in S than in L2 (F[1,7]=56.5, P<0.001). The data from this study therefore showed that in moderately-active females, a three-minute incremental protocol, allowing for the simultaneous calculation of the LT, could be used without compromising peak VO2, but that HRmax and Wpeak were affected.

Adolescent↗
PubMed1998

The critical power function is dependent on the duration of the predictive exercise tests chosen.

The linear relationship between work accomplished (W(lim)) and time to exhaustion (t(lim)) can be described by the equation: W(lim) = a + CP x t(lim). Critical power (CP) is the slope of this line and is thought to represent a maximum rate of ATP synthesis without exhaustion, presumably an inherent characteristic of the aerobic energy system. The present investigation determined whether the choice of predictive tests would elicit significant differences in the estimated CP. Ten female physical education students completed, in random order and on consecutive days, five all-out predictive tests at preselected constant-power outputs. Predictive tests were performed on an electrically-braked cycle ergometer and power loadings were individually chosen so as to induce fatigue within approximately 1-10 mins. CP was derived by fitting the linear W(lim)-t(lim) regression and calculated three ways: 1) using the first, third and fifth W(lim)-t(lim) coordinates (I135), 2) using coordinates from the three highest power outputs (I123; mean t(lim) = 68-193 s) and 3) using coordinates from the lowest power outputs (I345; mean t(lim) = 193-485 s). Repeated measures ANOVA revealed that CPI123 (201.0+/-37.9W) > CPI135 (176.1+/-27.6W) > CPI345 (164.0+/-22.8W) (P<0.05). When the three sets of data were used to fit the hyperbolic Power-t(lim) regression, statistically significant differences between each CP were also found (P<0.05). The shorter the predictive trials, the greater the slope of the W(lim)-t(lim) regression; possibly because of the greater influence of 'aerobic inertia' on these trials. This may explain why CP has failed to represent a maximal, sustainable work rate. The present findings suggest that if CP is to represent the highest power output that an individual can maintain "for a very long time without fatigue" then CP should be calculated over a range of predictive tests in which the influence of aerobic inertia is minimised.

Adenosine Triphosphate↗
PubMed1998

The relationship between plasma lactate parameters, Wpeak and 1-h cycling performance in women.

PURPOSE: The relationship between six descriptors of lactate increase, peak VO2, Wpeak, and 1-h cycling performance were compared in 24 trained, female cyclists (peak VO(2) = 48.11 +/- 6.32 mLxkg(-1)xmin(-1). METHODS: The six descriptors of lactate increase were: 1) lactate threshold (LT; the power output at which plasma lactate concentration begins to increase above the resting level during an incremental exercise test), 2) LT(1) the power output at which plasma lactate increases by 1 mM or more), 3) LT(D) (the lactate threshold calculated by the D-max method), 4) LT(MOD) (the lactate threshold calculated by a modified D-max method), 5) L4 (the power output at which plasma lactate reaches a concentration of 4 mmolxL(-1), and 6) LT(LOG) (the power output at which plasma lactate concentration begins to increase when the log ([La(-1]) is plotted against the log (power output). Subjects first completed a peak VO(2) test on a cycle ergometer. Finger-tip capillary blood was sampled within 30 s of the end of each 3-min stage for analysis of plasma lactate. Endurance performance was assessed 7 d later using a 1-h cycle test (OHT) in which subjects were directed to achieve the highest possible average power output. RESULTS: The mean power output (W) for the OHT (+/- SD) was 183.01 +/- 18.88, and for each lactate variable was:LT (138.54 +/- 46.61), LT(1) (179.17 +/- 27.25), LT(log) (143.97 +/- 45.74), L4 (198.09 +/- 33.84), LT(D) (178.79 +/- 24.07), LT(MOD)(212.28 +/- 31.75). Average power output during the OHT was more strongly correlated with all plasma lactate parameters (0.61<r<0.84) and W(peak) (r = 0.81) than with peak VO(2) (r = 0.55). The six lactate parameters were strongly correlated with each other (0.54<r<0.91) and of six lactate parameters, LT(D) correlated best with endurance performance (r = 0.84). CONCLUSIONS: It was concluded that plasma lactate parameters and W(peak) provide better indices of endurance performance than peak VO(2) and that, of the six descriptors of lactate increase measured in this study, LT(D) is most strongly related to 1-h cycling performance in trained, female cyclists.

Adult↗
PubMed1998

Membrane organization of bluetongue virus nonstructural glycoprotein NS3.

The smallest RNA segment (S10) of bluetongue virus (an orbivirus, family Reoviridae) encodes two closely related nonstructural proteins, the 229-amino-acid (aa) NS3 and the 216-aa NS3A. The proteins are found in glycosylated and nonglycosylated forms in infected cells (X. Wu, H. Iwata, S.-Y. Chen, R. W. Compans and P. Roy J. Virol. 66:7104-7112, 1992). The NS3/NS3A proteins have two hydrophobic domains (aa 118 to 141 and 162 to 182) and two potential asparagine-linked glycosylation sites (aa 63 and 150), one of which is located between the hydrophobic domains. To determine whether these features were used in the mature protein forms, we generated a series of mutants of the S10 gene and expressed them by using the vaccinia virus T7 polymerase transient-expression system. Our data indicate that both hydrophobic domains of NS3 span the cell membrane and that only the site at aa 150 is responsible for N-linked glycosylation of the NS3 proteins.

Amino Acid Sequence↗
PubMed1997

The use of lidocaine in microvascular reconstruction.

Vasospasm is known to have an adverse effect on the survival of free tissue transfers. Prolonged vasoconstriction decreases blood flow to the flap and promotes thrombosis at the anastomotic site. Because of its wide availability and rapid effect, topically applied lidocaine is used by many surgeons to prevent and correct vasospasm. Mucosal absorption of lidocaine is known to be rapid. Absorption by the surgical bed during microvascular reconstruction has not previously been determined. We had three specific aims: (1) to determine whether systemic absorption occurs, (2) if so, to determine the degree of absorption, and (3) to determine whether the type of reconstruction affects the degree of absorption. Twelve consecutive patients were entered into the study, four in a myocutaneous reconstructive group and eight in a fasciocutaneous group. Baseline serum lidocaine levels were drawn. After the initial application of lidocaine, levels were drawn every 30 minutes. Final levels were drawn 120 minutes after the last application. The total amount of lidocaine used ranged from 720 mg to 2600 mg. Application ranged from 7.5 mg/minute to 34.7 mg/minute (2 to 9 times the estimated toxic dose of 4 mg/minute for a patient weighing 70 kg). Results ranged from undetectable (<0.5 microg/ml) to minimal therapeutic levels (1.6 microg/ml). Toxic levels (>6.0 microg/ml) were not encountered. Significant (p < 0.05) differences were found between the fasciocutaneous group, which generally had no detectable serum levels, and the myocutaneous group, which had detectable subtherapeutic to minimally therapeutic levels. Doses considered toxic by intravenous or mucous membrane application may safely be applied topically. Greater absorption was found in the myocutaneous group, which is believed to be a result of the richer absorption surface provided by the muscular tissue.

Absorption↗
PubMed1997

Reliability of a 1-h endurance performance test in trained female cyclists.

Endurance performance is a common criterion used to evaluate training or dietary interventions. However, to accurately appraise the effects of an intervention, the endurance performance measure must be reliable. The purpose of the investigation was to establish the reliability of a 1-h endurance performance test. Twenty trained female subjects (peak VO2 = 47.4 +/- 7.2 ml.kg-1.min-1) completed two trials in which they had to generate the highest power output possible throughout 60 min of cycling. Heart rates (HR) and ratings of perceived exertion (RPE) were also recorded during these two trials. All tests were conducted on a wind-braked cycle ergometer set up to closely resemble the subject's own cycle. The trials were separated by 1 wk, conducted on the same day of the week, and completed at a similar time of the day. The average power outputs (+/-SD) for the two trials were 180.0 (+/-18.1) W and 180.0 (+/-20.6) W. The results revealed that average absolute power output, HR, and RPE were not significantly different between trials. The intraclass correlation coefficient (one way ANOVA) for average absolute power output was 0.97, the coefficient of variation was 2.7%, and the SEM was 3.4 W. These results suggest that under controlled conditions average absolute power output during a 1-h endurance test is a reliable measure for trained female cyclists.

Adult↗
PubMed1997

Ramp and constant power trials produce equivalent critical power estimates.

The standard critical power test protocol on the cycle ergometer prescribes a series of trials to exhaustion, each at a different but constant power setting. Recently the protocol has been modified and applied to a series of trials to exhaustion each at a different ramp incremental rate. This study was undertaken to compare critical power and anaerobic work capacity estimates in the same group of subjects when derived from the two protocols. Ten male subjects of mixed athletic ability cycled to exhaustion on eight occasions in randomized order over a 3-wk period. Four trials were performed at differing constant power settings and four trials on differing ramp incremental rates. Both critical power and anaerobic work capacity were estimated for each subject by curve fitting of the ramp model and of three versions of the constant power model. After adjusting for inter-subject variability, no significant differences were detected between critical power estimates or between anaerobic work capacity estimates from any model formulation or from the two protocols. It is concluded that both the ramp and constant power protocols produce equivalent estimates for critical power and anaerobic work capacity.

Adult↗
PubMed1997

Baculovirus-expressed nonstructural protein NS2 of bluetongue virus induces a cytotoxic T-cell response in mice which affords partial protection.

Virus-specific cytotoxic T lymphocytes were generated in two strains of mice (BALB/c and CBA/Ca) against baculovirus recombinant proteins (minor and nonstructural) derived from bluetongue virus serotype 10. Immunization of mice with recombinant baculovirus insect cell extracts expressing the nonstructural protein NS2 (Bac-NS2) conferred partial protection against infection with vaccinia virus expressing the NS2 protein. This protective immunity was mediated by CD8+ cells. In contrast, no protection was observed when mice were immunized with similarly expressed Bac-NS1 or -NS3 or the virion minor structural proteins (Bac-VP1, -VP4, or -VP6). Furthermore, the in vitro cytotoxicity activity of T cells derived from immunized animals did not correlate to the protective efficacy of baculovirus recombinant proteins. The implications of this work with regard to the design of noninfectious subunit vaccines are discussed.

Animals↗
PubMed1996

Lymphocytes from orally tolerized mice display enhanced susceptibility to death by apoptosis when cultured in the absence of antigen in vitro.

The mechanism responsible for the induction of immunological tolerance by oral administration of soluble antigen remains unclear. Here we show that, when cultured in vitro in the absence of antigen, lymphocytes from mice tolerized with a single feed of 25 mg of ovalbumin display an enhanced mortality in comparison with cells from immunized control animals. This increased cell death affects both CD4+ and CD8+ T-lymphocyte subsets, and morphological and flow cytometric analyses suggest that it occurs via apoptosis. All of the changes associated with the propensity of tolerant cells to die by apoptosis in vitro are reduced by the inclusion of the tolerizing antigen in the cultures. These results suggest that tolerance to dietary proteins is accompanied by functional changes in T lymphocytes that render them susceptible to apoptosis. This mechanism may underlie the profound and permanent tolerance to food antigens found under physiological conditions and may provide a useful basis for immunotherapy.

Administration, Oral↗
PubMed1996

The influence of resistance training on the critical power function & time to fatigue at critical power.

The present study examined whether a six-week resistance training program would influence the critical power (CP) function, time to exhaustion (TE) at CP and/or peak oxygen uptake (VO2 peak). The CP function is believed to provide an index of endurance ability (CP given by the slope), and anaerobic work capacity (the y-intercept). Eight healthy, untrained males undertook lower-body resistance training (90 min/day, 3-4 times/wk) for six weeks; eight controls refrained from resistance or endurance training for the same period. Before and immediately following the training period, subjects completed three trials to determine their CP function, a test of VO2 peak, a one-repetition maximum (1-RM) leg press test and TE at their CP. Training significantly increased both 1-RM leg press (28.6%, P < 0.05) and the y-intercept (34.9%, P < 0.05) while no changes in CP, VO2 peak or TE (p > 0.05) were found. Changes in the y-intercept following resistance training were negatively correlated with changes in the CP (r = -0.94, p < 0.05, N = 8). The present data show that the y-intercept of the CP function is sensitive to, and modified by, six weeks of resistance training. Given that resistance training had no significant influence on CP, TE at CP or VO2 peak, the present study has also shown that six weeks of resistance training will not alter indices of endurance ability. The negative relationship between changes in the y-intercept and CP exposes a potential limitation of the linear CP function when evaluating changes in endurance ability following an intervention which significantly alters the y-intercept.

Adolescent↗
PubMed1995

Effect of end-point cadence on the maximal work-time relationship.

This study examined the effect of end-point cadence on the parameters of the work-time relationship determined for cycle ergometry. Eight male subjects completed four maximal tests on an electrically-braked cycle ergometer that regulated a constant power output independent of cadence. The power outputs imposed ranged between an average of 259 W and 403 W, whereas the corresponding durations ranged between 139 s and 1691 s. During each test subjects were required to maintain a cadence of 80-90 rpm. Accumulated time to end-point cadences of 70, 60 and 50 rpm were recorded. The four work-time determinations for each of three end-point cadences were used to determine linear relationships between work and time, yielding both a y-intercept, which represents anaerobic work capacity, and a slope, which is termed critical power (CP), for each end-point cadence. There was a significant increase in the y-intercept as end-point cadence decreased from 70 to 60 rpm (F[1,7] = 36.7, p < 0.001) or 70 to 50 rpm (F[1,7] = 80.1, p < 0.001), but not from 60 rpm to 50 rpm (F[1,7] = 3.28, p > 0.05). In contrast, there was no effect of end-point cadence on CP (F[2,14] = 1.89, p < 0.05). These results demonstrate that the end-point cadence selected to terminate tests only affects the y-intercept of the work-time relationship. To control for this effect, the cadence at which each test is terminated should be standardised if determination of anaerobic work capacity, as represented by the y-intercept, is required.

Adult↗
PubMed1995

The influence of recovery duration between periods of exercise on the critical power function.

It has been shown that when three periods of exercise at different intensities are performed to exhaustion, the linear regression of the work accomplished on the time taken defines the critical power (CP) function. The slope of this function is related to endurance ability, whereas the y-intercept is considered to represent anaerobic work capacity (AWC). The purpose of this study was to determine whether two different recovery durations (3 and 24 h), separating three periods of exercise, would elicit differences in the linear CP function. Nine healthy, untrained female students [19.5 (SD 1.6) years] completed five sessions of cycle exercise to exhaustion in random order (familiarisation). Three of these five power outputs were then used in the main part of the study in which the subjects were randomly assigned to two groups. Group one first performed the three power outputs on the same day, with 3 h of passive recovery separating each session (3 on 1). Group two first performed the three power outputs on 3 consecutive days, with approximately 24 h between sessions (3 con). Following 1 day of rest, group one repeated their three power outputs on 3 consecutive days and group two completed their three tests on the same day. Repeated-measures ANOVA revealed no significant differences between the mean values of CP or AWC calculated from the 3 on 1 or 3 con conditions. Trial two estimates of CP were, however, 3.4% (P < 0.05) higher than trial one (familiarisation) estimates. The results of this study showed that reliable measurements of both CP and AWC can be determined from three tests separated by rest periods of 3 h, provided that the subjects are first familiarised with the tests. We found one series of five exercise sessions to be sufficient familiarisation to ensure similar subsequent estimates of CP.

Adult↗
PubMed1994

Why and when do some language-impaired children seem talkative? A study of initiation in conversations of children with semantic-pragmatic disorder.

Six language-impaired children fitting the clinical picture of semantic-pragmatic disorder (mean age 11 years) engaged in conversations with adults in four situations varying in terms of familiarity of the interlocutor (familiar or unfamiliar) and type of setting (interview or toy exploration). These children did not produce more utterances or longer utterances than normally developing children of similar age or ability, but they were more likely to produce utterances that served the conversational function of initiating, rather than responding or acknowledging. This tendency was most pronounced in the toy setting. There was a nonsignificant trend for control children to initiate more with a familiar than with an unfamiliar adult, but no such tendency in the semantic-pragmatic group. A high rate of initiations in children with semantic-pragmatic disorder cannot be regarded as an unusual behavior provoked by the demands of the interview setting, as it is even more apparent during toy exploration, where the child is under less pressure to respond to adult questions.

Adult↗