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Biomedical subjects

D Bailey

Publications and source records attributed to D Bailey.

At least 127 records · Page 7Linked to original sources
PubMed1989

Chloroplast thylakoid protein phosphatase is a membrane surface-associated activity.

Chloroplast thylakoid protein phosphatase activity was measured using (32)P-labeled histone as an exogenous substrate and an assay of the (32)Pi released involving formation of a phosphomolybdate complex and organic extraction. The activity was liberated from wheat (Triticum aestivum) thylakoids by washing the membranes in NaCl-containing solutions followed by centrifugation. The liberated phosphatase activity had a pH optimum of approximately 6.75, was inhibited by addition of 10 millimolar EDTA or EGTA, and was stimulated by addition of millimolar amounts of dithiothreitol, magnesium, manganese, or calcium ions. The rate of thylakoid protein dephosphorylation was decreased following liberation of a portion of the protein phosphatase activity and was increased by addition of salt-liberated phosphatase fraction. These results suggest that at least a portion of wheat thylakoid protein phosphatase is a peripheral, rather than an integral, membrane protein.

Journal Article↗
PubMed1989

A novel maturation marker for human Sertoli cells.

The maturation of Sertoli cells at puberty is critical for the initiation and maintenance of spermatogenesis. Little is known about the factors which control Sertoli cell maturation. We have examined Sertoli cells in testicular specimens from 44 subjects, ranging in age from 6 months to 67 years, for reactivity with a mouse monoclonal antibody (M2A) using the immunoperoxidase reaction. We found positive reactivity with prepubertal but not postpubertal Sertoli cells, suggesting that the antigen defined by M2A was a marker of immature Sertoli cells. This marker may be useful for studying the factors which influence Sertoli cell maturation during development of the testis.

Adolescent↗
PubMed1989

Referral to child psychiatry: parent and doctor motives and expectations.

We studied general practitioner and parental attitudes towards the referral to child psychiatric clinics of 65 children aged 7-12 yrs. The doctors gave parental request and anxiety and the seriousness of the child's condition as the most common reasons for the referral. Their expectations of it were well in line with current psychiatric practice. There was a wide variety of parental motivations in attending the surgery, and the expectations of the referral were similarly diverse. Most prominent though were help for the child and a need to know underlying reasons. Although three-quarters of parents were satisfied with the general practitioner intervention, the majority said that they had not been given information about the psychiatric assessment process, nor knew what to expect from it, and three-quarters of the children were referred after only one consultation.

Child↗
PubMed1989

Psychiatric disorders in general paediatric referrals.

Detailed interviews with parents of 128 children aged 7 to 12 years consecutively referred to general paediatric clinics identified psychiatric disturbance in 36 (28%) of the children. Emotional disorders were the commonest psychiatric diagnoses (present in two thirds); less frequent diagnoses were conduct disorders (5/36, 14%), mixed conduct/emotional disorders (six, 17%), and hyperkinetic syndrome (three, 8%). Disturbance was related to level of energy, with disturbed children being described significantly more frequently as 'bounding with energy' or conversely 'tired and apathetic'. Psychosocial problems (broken homes, mentally distressed mothers, family stress including financial stress and marital difficulty) were also increased in the disturbed group, but most of all these mothers reported feeling stressed in relation to the children. Psychiatric disorders, particularly emotional disorders, are common associated problems in paediatric referrals. Family stress, specially that focused on parenting, is likely to be an important factor contributing to disturbed children's consultations in general paediatric clinics.

Affective Symptoms↗
PubMed1988

A monoclonal antibody as a marker for carcinoma in situ germ cells of the human adult testis.

Carcinoma in situ of the testis (CIS) is a precursor of invasive testicular germ cell tumours. The diagnosis of CIS is however often missed when conventional histological techniques are used. No specific immunological marker for CIS germ cells of the testis has been demonstrated previously. A novel monoclonal antibody, M2A, reacting with malignant germ cells of seminomas has recently been developed. Using the immunoperoxidase reaction on tissue sections, we tested the reactivity of M2A with CIS germ cells of the human adult testis. Positive reaction was found in 19 of 20 testicular specimens showing CIS, whereas no staining was found in 39 testicular biopsies without CIS. Thus, M2A may serve as a diagnostic marker in detection of CIS germ cells.

Adult↗
PubMed1988

Use of anti-seminoma monoclonal antibody to confirm the diagnosis of mediastinal seminoma. A case report.

Chest x-ray in a 20-year-old man showed a large anterior mediastinal mass and a needle aspirate was diagnosed by light microscopy (LM) as non-Hodgkin's lymphoma. Treatment with CHOP (cyclophosphamide, adriamycin, vincristine and prednisone) was ineffective and a tissue biopsy was performed. LM showed large, non-cohesive cells with abundant cytoplasm and rounded nuclei. Differential diagnoses included malignant lymphoma, seminoma, thymoma, anaplastic carcinoma, malignant melanoma and paraganglioma. Electron microscopy was not conclusive and immunoperoxidase staining was carried out. The malignant cells were negative for common leukocyte antigen, Leu M1, alpha-fetoprotein, chorionic gonadotrophin, cytokeratin, epithelial membrane antigen, carcinoembryonic antigen, S-100 protein and neuron-specific enolase but positive for placental alkaline phosphatase. In addition, there was strong positivity with a monoclonal antibody (mAb) which was recently shown to react with testicular seminomas. This case illustrates the value of this mAb in confirming the diagnosis of mediastinal seminoma.

Adult↗
PubMed1988

Child and family factors associated with referral to child psychiatrists.

We compared children aged 7-12 years referred to child psychiatrists by general practitioners (GPs) with community control children matched for the presence of psychiatric disorder. Referral status in psychiatrically disturbed children was linked to male sex, severity of the disorder, mental problems in the mothers, high levels of psychosocial stress in the family, and less support from extended families. For the whole group of referred children, the referral was associated with high antisocial scores on parental questionnaires, with parental reports of problems in controlling the children, and of high levels of stress felt in relation to them. In addition, parents of referred children were in disadvantaged socioeconomic groups.

Affective Symptoms↗
PubMed1988

Chemotherapy following surgery for stages IE and IIE non-Hodgkin's lymphoma of the gastrointestinal tract.

Twenty-six patients were treated with chemotherapy following surgery for gastrointestinal non-Hodgkin's lymphoma (GI-NHL). The median age was 50 years (range, 20 to 76). The primary site included stomach (16 patients), small bowel (seven), large bowel (two), and mesenteric nodes (one). Following surgery, nine patients had macroscopic and four patients had microscopic residual disease, and 13 were felt to have had complete surgical resection. Thirteen patients were stage I and 13 were stage II. Sixteen patients were treated with COPP (cyclophosphamide, vincristine, procarbazine, prednisone), nine with CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone), and one with CVP (cyclophosphamide, vincristine, prednisone). At a median follow-up of 50 months (8+ to 178+ months) ten of 12 stage I patients and nine of 14 stage II patients remain alive. Of the nine patients with macroscopic residual disease, four died of disease 6.5 to 11.0 months after diagnosis, and five are alive 8+ to 178+ months from diagnosis. Fourteen of the remaining 17 patients who had complete surgical resection are alive without disease. Death in the other three patients was due to multiple abdominal abscesses at 12 months, adenocarcinoma of the colon at 57 months, and dementia and progressive neurologic dysfunction at 65 months. No patient who had complete resection has relapsed or developed systemic disease after chemotherapy. These results suggest that complete surgical resection is an important prognostic factor and that chemotherapy without irradiation in completely resected localized GI-NHL can prevent local and systemic relapse resulting in long-term disease-free survival.

Adult↗
PubMed1988

Prevention of spontaneous but not of adoptively transferred diabetes by injection of neonatal BB/hooded hybrid rats with splenocytes or concanavalin A blasts from diabetes-free strains.

Spontaneous diabetes was fully prevented in 65 BB/hooded (BB/h) highly diabetes-prone hybrid rats that were given five intraperitoneal injections (25 to 30 x 10(6) cells/injection) of fresh splenocytes or concanavalin A (ConA)-activated cultured splenocytes (blasts) from the diabetes-free Wistar-Furth or Long-Evans strains during the first 2 postnatal wk. Rats remained under observation for up to the age of 180-200 days. Of 70 littermate controls that received no cell injections, 63 developed overt diabetes before the age of 180 days. One intraperitoneal injection (25 x 10(6) cells) of splenocytes or blasts given during the first 36 h after birth was not as effective as multiple injections in preventing overt diabetes. Mild insulitis was present in 4 of 59 "protected" rats; small, discrete mononuclear infiltrates in periductular connective tissue and/or between pancreatic acini were observed in 27. Nondiabetic BB/h rats that were protected with splenocytes or blasts from diabetes-free strains had the same degree of lymphopenia in peripheral blood and spleen as age-matched, insulin-treated diabetic BB/h rats, but the level of islet cell surface antibodies in their serum was significantly lower. The same neonatal injections that protected rats from the development of spontaneous diabetes were completely ineffective in preventing the adoptive transfer of diabetes later in life by the injection of blasts from acutely diabetic BB/h rats.

Acute Disease↗
PubMed1988

Death of a child associated with multiple overdoses of acetaminophen.

A case of long-term acetaminophen overdosage in a six-year-old child, which contributed to her death despite optimal medical management including oral acetylcysteine therapy, is reported. Acetaminophen 325 mg every six hours was prescribed for fever associated with measles. Believing that acetaminophen was nontoxic, the child's mother progressively increased the dose over three days, first in response to fever and subsequently for abdominal pain probably secondary to unrecognized acetaminophen toxicity. On admission to the hospital, the patient's serum acetaminophen concentration was 163 micrograms/mL (11 hours after the last dose); subsequently, the acetaminophen half-life was determined to be 15 hours. A course of oral acetylcysteine therapy (a loading dose of 140 mg/kg as the sodium salt followed by 70 mg/kg every four hours for 17 doses) was begun. Hepatic and renal failure developed within two days, followed by the onset of seizures, and brain death occurred on the 11th day. Autopsy findings consistent with acetaminophen toxicity included centrilobular hepatic and renal tubular necrosis. Aspergillis fumigatus and Cryptococcus neoformans isolates from pulmonary abscesses and bronchopulmonary lymph nodes, respectively, were an unexpected finding. However, in the absence of acetaminophen overdosage, death would have been unlikely. Cryptococcal lymphadenitis was believed to have been the initial febrile illness that was treated with supratherapeutic doses of acetaminophen. Fatalities in children from a single overdose of acetaminophen have been rare, and there is only one previous report of a fatality after long-term administration of multiple excessive doses. The lethal outcome in this case illustrates the need to educate the public on the potential toxicity of nonprescription medications.

Acetaminophen↗
PubMed1987

A rapid method for the evaluation of diagnostic radioaerosol delivery systems.

The effective delivery (ED) in MBq/min of a 100 MBq/ml nebuliser solution was defined as the rate of delivery of droplets in the respirable size range (aerodynamic diameter below 3.3 micron) to the mouthpiece of the aerosol delivery system (ADS). Wasted delivery (WD) was defined as the rate of delivery of droplets above 3.3 microns. ED and WD were measured on four types of commercially available ADS. The aerosols were sampled at the mouthpiece of each system and droplet size distribution measured with a seven stage cascade impactor. The effect of ambient air humidity on the droplet size produced by the Cadema delivery system was also evaluated. The ED values ranged from 6 to 15 MBq/min and WD values from 0.01 to 15 MBq/min. Two ADS produced low ED and WD values (6.1-9.0 and 0.01-0.07 MBq/min, respectively) due to a low output, while another produced higher ED and WD values [11.3 +/- 0.2 (SD) and 15.4 +/- 0.5 (SD) MBq/min, respectively] due to a larger droplet size. The Cadema delivery system gave the optimum characteristic of high ED [13.9 +/- 0.8 (SD) MBq/min] and low WD [1.24 +/- 0.61 (SD) MBq/min] values. The mass median aerodynamic diameter (MMAD) of the Cadema ADS fell by 22% (P less than 0.01) as the ambient dilution air was dried from a high relative humidity (RH) (88%-100%) to a low RH (12%-17%). The variability of both MMAD and geometric standard deviation (sigma g) was increased with dry dilution air (P less than 0.01).

Aerosols↗
PubMed1987

Psychosomatic aspects of children's consultations in primary care.

Family doctors identified psychological factors associated with the presenting complaint in 17% of children aged 7 to 12 years attending their care. When compared with other children attending within the same age group, children said by their doctors to show associated psychological factors tended to present with psychosomatic-type symptoms (bed-wetting, asthma, skin rashes, abdominal pains, blackouts and headaches) and to make more use of medical services (both hospital and primary care). Our findings suggest that the families from which these children came had relatively high surgery attendance rates and more mothers with health problems. More of these families were regarded by the doctors as being under stress, and there were indications that concern about the children and their schooling was particularly common amongst the parents. Exploration of parental concerns about their children's health and about educational expectations would appear specially indicated in these cases.

Child↗
PubMed1986

The accuracy of dating childhood life events on a parental questionnaire.

The accuracy in dating events by parents on the Coddington childhood life-event self-report inventory was assessed, by examining the concordance between events reported on the inventory and at a subsequent life-events interview. Seventy per cent of all the events reported on the inventory were dated at interview within the stated three month period. On group comparisons of all events reported, concordance in dating was not significantly related to the nature of the events classified according to desirability, specificity or direction (exit/entrance). Nor was it related to psychological deviance in the mother or in the child. However, when statements in the questionnaire were examined individually for concordance, there was full concordance for desirable events and for hospitalizations. In contrast, there was no concordance when marital separation, remarriage and birth of a sib were reported. The reasons for this are explored.

Child↗