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Biomedical subjects

D Aubert

Publications and source records attributed to D Aubert.

158 records · Page 9Linked to original sources
PubMedDate not supplied

Characterization of specific anti-Candida IgM, IgA and IgE: diagnostic value in deep-seated infections.

The proposed serological diagnosis of systemic Candida infections is based on a microplate immunocapture technique detecting IgM, IgA and IgE anti-Candida antibodies. Activity is revealed with a suspension of human erythrocytes sensitized with somatic antigen of Candida albicans, and is quantified on an automated plate reader. The sera were obtained from patients with deep-seated (n = 56) and superficial (n = 193) candidosis. We compared this immunological method with a combination of indirect immunofluorescence and co-immunoelectrodiffusion. The immunocapture method was more sensitive (80.4% vs. 48.2% with indirect immunofluorescence and 58.9% with co-immunoelectrodiffusion), and often provided the diagnosis at an earlier stage, with clear therapeutic advantages. The IgA isotype was a particularly valuable marker of deep-seated Candida infections.

Adolescent↗
PubMedDate not supplied

Description and analysis of allele distribution for four VNTR markers in French and French Canadian populations.

Allele distributions were determined for several VNTR loci in the general French Caucasian population, a French regional population from Brittany and in three French Canadian populations, residing either in Montréal or in one of two other regions in Québec. Allele distributions are highly polymorphic in all populations sampled. Despite a well-documented genetic founder effect in one of the populations, no disequilibrium was detected over all genotypes, within and between loci, for the data bases in this study. Moreover, there were no forensically significant differences observed between estimated frequencies of 1,964 Caucasian and non-Caucasian target DNA profiles estimated in any of the groups.

Alleles↗
PubMedDate not supplied

Frequency of specific anti-Toxoplasma gondii IgM, IgA and IgE in colombian patients with acute and chronic ocular toxoplasmosis.

We studied the frequency of specific anti-Toxoplasma IgM, IgA and IgE antibodies in serum of 28 immunocompetent Colombian patients, selected by ophthalmologists and with lesions that were compatible with ocular toxoplasmosis. Patients were classified in three groups: (i) group 1 consisted of ten patients with a first episode; (ii) group 2, with seven patients with a recurrence and (iii) group 3, consisted of eleven patients with chronic chorioretinal lesion without uveitis. We found that 10/28 (35%) of Colombian patients with ocular toxoplasmosis possessed at least one serological marker for Toxoplasma infection different from IgG. In group 1 (first episode), we found simultaneous presence of specific IgM plus IgA plus IgE in 1/10 (10%). In group 2 (recurrences) in 1/7 (14%) we found IgM and IgA test positives and in 1/7 (14%) we found IgM and IgE tests positives. In group 3 (toxoplasmic chorioretinal scar) the IgA serological test was positive in 2/11 (18%). These results show that serum IgM or IgA or IgE can be present during recurrences.

Acute Disease↗
PubMedDate not supplied

[Spontaneous perirenal hematoma in infants. Apropos of 2 cases].

The authors report two personal cases and note the special appearance of perirenal hematomas in infants. The pathogenesis is often obscure but this condition should be classified with the spontaneous perirenal hematomas and retroperitoneal tumour masses in infants.

Adrenal Gland Diseases↗
PubMedDate not supplied

[Infant esophageal pH monitoring (author's transl)].

The authors expound their experience of the infant esophageal pH monitoring from a 100 cases statistic. The digestive radiography is necessary for the vomiting infants but the efficiency of radiology is inadequate to diagnose the reflux and particularly to give a sufficient assessment of its intensity. The esophageal pH monitoring performed during a period of 3 hours is a tremendous learning experience; is allows the clinician to take mild reflux and severe reflux into account; performed in several positions, it shows interesting variations that we can turn to account by giving the therapeutic advices. That test is innocuous, easy, little costly, easily repeated; it must have its place beside other investigations and make us advance in the knowledge of a so often found disorder development.

Bronchitis↗
PubMedDate not supplied

[Post-traumatic stenosis of the bronchus (author's transl)].

The authors describe the unusual symptomatology presented by 2 cases of traumatic bronchial rupture in childhood. The first concerns a boy with rupture of the left superior bronchus revealed by a contra-lateral hemopneumothorax. The second cases concerns a young girl with rupture of the right main bronchus: wheezing appearing on the tenth day permitted the diagnosis. They emphasize the eventuality of partial bronchial lesions in cases of moderate and serious thoracic trauma in the child, which should be investigated by bronchoscopy the slightest doubt exists.

Adolescent↗
PubMedDate not supplied

[Characterization of specific IgG, IgM, IgA and IgE isotypes in profound candidiasis].

Enzyme-linked immunofiltration assay technique (Elisa) has been applied to the characterization of G, M, A and E anti-Candida antibodies isotypes specific to cell wall mannans in 201 sera from 126 patients. These sera were studied at the same time using Co-immunoelectrodiffusion and indirect immunofluorescence. In 18 of 21 patients with systemic candidiasis, Elisa demonstrated the presence of antimannan IgG antibodies in sera contemporary of Candida positive blood culture. These IgG were associated with antimannan IgM, A and E in 15 patients. In 37 patients colonized with Candida, used as negative controls, antimannan IgG were detected in 3 cases, and in 2 were associated with specific IgMs. The sensitivity and specificity of Elisa IgM and IgA in the diagnosis of systemic Candidiasis were 85.7% and 81%, respectively. The kinetic study shows that the different isotypes appeared most of the time simultaneously. The evolution of the 4 isotypes beyond the acute episode was variable and without correlation with the clinical status. The decrease of IgG was slower than the one of IgM, IgA or IgE. The systematic research, in at risk patients, of antimannan antibodies using Elisa required simple technology. A simple method should allow to aim at other functional antigens which could be used in a quantitative manner to determine the efficacy of the medical treatment.

Adolescent↗